Method for rapid detection of contents of water and protein in royal jelly

A technology for protein content and royal jelly, which is used in measuring devices, analyzing materials, and analyzing materials by optical means, can solve problems such as labor-intensive, time-consuming, and complicated operations, and achieves reduction of random errors, simple operation, and real-time monitoring. fast effect

Active Publication Date: 2015-08-19
BEE RES INST CHINESE ACAD OF AGRI SCI
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Problems solved by technology

[0010] In order to solve the shortcomings of complex operation, time-consuming and labor-intensive detection methods for the moisture and protein content of royal jelly, the present invention provides a method for quickly determining the moisture and protein content of royal jelly by combining mid-infrared spectroscopy technology with the principle of partial least squares

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  • Method for rapid detection of contents of water and protein in royal jelly
  • Method for rapid detection of contents of water and protein in royal jelly
  • Method for rapid detection of contents of water and protein in royal jelly

Examples

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Embodiment 1

[0060] Example 1: The establishment of a rapid assay method for moisture and protein content of royal jelly

[0061] 1. Determination of water content and protein content of samples A total of 51 propolis samples were collected directly from bee farms. The determination of moisture and protein content in royal jelly is carried out according to the method of national standard GB9697-2008 (determination method of moisture content in royal jelly-reduced pressure drying method; determination of protein content-Kjeldahl method). in:

[0062] The determination method of moisture content in royal jelly is:

[0063] Take more than 0.5g of royal jelly sample, put it in a weighing bottle that has been dried to a constant weight, accurately weigh it, flatten it, put it into a pressurized drying oven, and put it in a pressurized drying oven at a temperature of 75°C and a pressure of -0.095MPa~-0.10MPa (-730mmHg ~-760mmHg) after drying for 4 hours, take out the weighing bottle, put it in...

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Abstract

The invention relates to a method for rapid detection of the contents of water and protein in royal jelly. The method comprises the following steps: 1, determination of the contents of water and protein in a royal jelly sample by using a conventional method; 2, establishment of an infrared spectrum database of the royal jelly sample: a step of scanning the royal jelly sample by using a Fourier transform infrared spectrometer, wherein each sample is scanned 3 times and an average value is taken; 3, selection of a spectral pretreatment method and an optimal modeling band; and 4, establishment of a quantitative model of the water and protein on the basis of optimal parameters. The method provided by the invention has simple operation and low cost and can quickly and reliably predict the contents of water and protein in the royal jelly on the premise that identification accuracy is ensured.

Description

technical field [0001] The invention relates to a rapid detection method for the quality of royal jelly, in particular, it is a method for measuring the content of water and protein by using the mathematical model between mid-infrared spectrum of royal jelly and the water and protein of royal jelly. Background technique [0002] Royal Jelly (Royal Jelly), also known as royal jelly, royal jelly, etc., is a milky substance with complex chemical composition secreted by the tongue glands and mandibular glands of worker bees in the bee colony. It is the food for the larvae that will become queen bees. , is also the lifelong food of the queen bee. A large number of studies at home and abroad have shown that royal jelly is rich in active substances and is a natural health food suitable for human beings. [0003] The content of moisture and protein in royal jelly are important indicators to evaluate its quality, and protein is one of the main substances affecting the biological act...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/3577
Inventor 陈兰珍杨娟薛晓锋
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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