Total bacterial count test piece and manufacturing method thereof

A technology for the total number of colonies and test strips, which is applied in biochemical equipment and methods, microbial measurement/inspection, biochemical instruments, etc., and can solve the problems that the compliance of bacterial growth needs to be improved.

Inactive Publication Date: 2017-03-22
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still some deficiencies, especially in terms of bacterial growth compliance.

Method used

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  • Total bacterial count test piece and manufacturing method thereof
  • Total bacterial count test piece and manufacturing method thereof

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Embodiment Construction

[0029] specific implementation

[0030] In order to understand the present invention, the present invention will be further described below in conjunction with embodiment: following embodiment is illustrative, not limiting, can not limit protection scope of the present invention with following embodiment.

[0031] 1. The main optimization process of the test piece structure

[0032] (1) Feasibility of different carrier structures

[0033] By referring to the relevant literature, combined with the collected original materials for the primary screening of the carrier structure, including filter paper, non-woven fabric, combination of non-woven filter paper, filter paper combined with filter membrane, non-woven combined filter membrane, non-woven combined filter paper and Feasibility assessment of various structures such as filter membranes. After primary screening, four types of structures that may be used as carriers were selected for secondary screening. The first type is a...

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Abstract

The invention relates to a total bacterial count test piece and a manufacturing method thereof. The total bacterial count test piece comprises a carrier structure and further includes two layers of gaskets, an upper layer base material and a lower layer base material. The carrier structure is formed by combining a nonwoven and a filter film. The total bacterial count test piece can contain 500 [mu]l of liquid. The total bacterial count test piece manufactured through the manufacturing method can count red bacterial colonies after a liquid sample is inoculated and cultivation is conducted under the condition of 37 DEG C for 48 h. Through the contrast experiment result of a test piece method and a national standard method, the effects can be known that the experimental bacteria detection conformity of the test piece can reach 105%, and the practical sample total bacterial count detection conformity of the test piece can reach 89% or above. Bacterial colony counting is conducted through the test piece, operation is simple and convenient, bacterial colony forms are good, and results are accurate.

Description

technical field [0001] The invention belongs to the technical field of food detection and specifically designs a test piece for the total number of bacterial colonies, a preparation method and its application in detecting the total number of bacterial colonies in food. [0002] technical background [0003] The total number of bacterial colonies in food is an important indicator in food hygiene testing, and it is a mark to determine the degree of food contamination by bacteria. The classic method of microbiological detection - plate pouring method has good accuracy, but the operation is cumbersome and has certain professional requirements for counting personnel. Microbiological detection test strips emerged as the times require, using non-woven fabrics, filter membranes or cold water soluble colloids as the carrier of the culture medium, and directly dropping the sample solution to screen, cultivate and count the target bacteria in a targeted manner. The biggest advantage is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12Q1/06
CPCC12Q1/06
Inventor 杜欣军徐艺芮李萍王硕
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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