Total bacterial count test piece and manufacturing method thereof

A technology for the total number of colonies and test strips, which is applied in biochemical equipment and methods, microbial measurement/inspection, biochemical instruments, etc., and can solve the problems that the compliance of bacterial growth needs to be improved.
CN106520542AInactive Publication Date: 2017-03-22TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Publication Date
2017-03-22
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

The invention relates to a total bacterial count test piece and a manufacturing method thereof. The total bacterial count test piece comprises a carrier structure and further includes two layers of gaskets, an upper layer base material and a lower layer base material. The carrier structure is formed by combining a nonwoven and a filter film. The total bacterial count test piece can contain 500 [mu]l of liquid. The total bacterial count test piece manufactured through the manufacturing method can count red bacterial colonies after a liquid sample is inoculated and cultivation is conducted under the condition of 37 DEG C for 48 h. Through the contrast experiment result of a test piece method and a national standard method, the effects can be known that the experimental bacteria detection conformity of the test piece can reach 105%, and the practical sample total bacterial count detection conformity of the test piece can reach 89% or above. Bacterial colony counting is conducted through the test piece, operation is simple and convenient, bacterial colony forms are good, and results are accurate.
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Description

technical field

[0001] The invention belongs to the technical field of food detection and specifically designs a test piece for the total number of bacterial colonies, a preparation method and its application in detecting the total number of bacterial colonies in food.

[0002] technical background

[0003] The total number of bacterial colonies in food is an important indicator in food hygiene testing, and it is a mark to determine the degree of food contamination by bacteria. The classic method of microbiological detection - plate pouring method has good accuracy, but the operation is cumbersome and has certain professional requirements for counting personnel. Microbiological detection test strips emerged as the times require, using non-woven fabrics, filter membranes or cold water soluble colloids as the carrier of the culture medium, and directly dropping the sample solution to screen, cultivate and count the target bacteria in a targeted manner. The biggest advantage is...

Claims

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