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Low temperature resistance straw mushroom bacterial and breeding method thereof

A technology of low temperature resistance and strain, applied in the field of bioengineering, to achieve the effects of good genetic stability, improved yield and quality, and expanded planting seasons

Active Publication Date: 2013-12-11
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the application of genome rearrangement technology to breed strains resistant to low temperature

Method used

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  • Low temperature resistance straw mushroom bacterial and breeding method thereof
  • Low temperature resistance straw mushroom bacterial and breeding method thereof
  • Low temperature resistance straw mushroom bacterial and breeding method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] (1) Construction of starting strain library

[0058] 16 Strains of Volvariella volvacea (V23, V9715, V9, V106, V97, V874, V5-2, VW, VG, VT-1, VT-2, V14, NO3, VB2, V28, V5), constructed a multi-genotype Starting strain library, the constructed starting strain library is rich in genotypes, including genotypes currently used in production. See Table 1 for the sources of the 16 starting strains.

[0059] Table 1 Departure strains and their sources

[0060]

[0061]

[0062] (2) Protoplast preparation

[0063] Inoculate 16 starting strains on PDA solid medium, culture at a constant temperature of 32±0.5°C for 3 to 4 days, take vigorously growing hyphae on PDA medium, inoculate in PDA liquid medium, and culture at 32±0.5°C , shake once a day in the morning and evening. The hyphae cultivated for 2 days were washed with sterile water, and then the surface moisture was sucked off with sterile filter paper for later use. Lyticase (purchased from Guangdong Institute of ...

Embodiment 2

[0093] (1) Construction of starting strain library

[0094] The construction of the starting strain library is the same as in Example 1.

[0095] (2) Protoplast preparation

[0096] Protoplast preparation method is the same as embodiment 1. The differences from the parameters in Example 1 are: the culture time of the mycelium liquid is 1d; when the mycelium is enzymatically hydrolyzed, 1mL of enzyme solution is added for every 150mg of mycelia for enzymolysis, and the enzymolysis time is 1.5h; the centrifugation speed of the filtrate is 3500r / min .

[0097] (3) Protoplast mutagenesis and mutant strain screening

[0098] The purified protoplasts of each starting strain were equally divided into 4 parts, which were subjected to electron beam mutagenesis, 60 C o - gamma mutagenesis, ultraviolet mutagenesis, EMS mutagenesis, screening of low-temperature-resistant mutant strains after mutagenesis, the specific steps are the same as in Example 1. The difference with the parame...

Embodiment 3

[0111] (1) Construction of starting strain library

[0112] The construction of the starting strain library is the same as in Example 1.

[0113] (2) Protoplast preparation

[0114] Protoplast preparation method is the same as embodiment 1. The differences from the parameters in Example 1 are: the culture time of the mycelium liquid is 3 days; when enzymatically hydrolyzing the mycelium, add 1 mL of enzyme solution for every 250 mg of mycelia for enzymatic hydrolysis, and the enzymatic hydrolysis time is 4 hours; the centrifugation speed of the filtrate is 3000 r / min.

[0115] (3) Protoplast mutagenesis and mutant strain screening

[0116] The purified protoplasts of each starting strain were equally divided into 4 parts, which were subjected to electron beam mutagenesis, 60 C o - gamma mutagenesis, ultraviolet mutagenesis, EMS mutagenesis, screening of low-temperature-resistant mutant strains after mutagenesis, the specific steps are the same as in Example 1. The differe...

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Abstract

The invention relates to low temperature resistance straw mushroom bacterial and a breeding method thereof, wherein starting bacterial library construction, straw mushroom protoplast preparation and mutagenesis, mutation bacterial library construction, and straw mushroom protoplast inactivation and fusion are performed to finally screen rearrangement bacterial with characteristics of low temperature resistance maintaining, no significant growth speed change, no significant colony morphology change and no significant color change to obtain the low temperature resistance bacterial, wherein the biological preservation number is CCTCC NO:M2013207. The low temperature resistance straw mushroom has the following characteristics that: normal fruiting can be achieved, genetic stability is good, mycelium has vitality after storing for 42 h at a temperature of 0 DEG C, a fruiting body can be stored for 28 h at a temperature of 10 DEG C, low temperature resistance of the straw mushroom fruiting body is substantially increased, and the difficult problem that the existing straw mushroom fruiting body can not subjected to low temperature preservation is solved so as to substantially increase a yield and quality of the straw mushroom, effectively expand the straw mushroom planting season, and improve economic benefits.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a low-temperature resistant straw mushroom strain and a breeding method thereof. Background technique [0002] Straw mushroom [Volvariella volvacea (Bull.ex Fr.) Sing.] originated in China, also known as Chinese mushroom, belongs to Basidiomycotina, Laminomycetes, Non-septabasidiomycetes, Agaricaceae, Amanita Branch, the genus Villosa genus. Straw mushroom is a kind of grass-rot fungus that likes humidity and temperature. It has a short production cycle and low cost. It is widely cultivated in tropical and subtropical high-temperature and rainy regions. Straw mushrooms are mainly sold fresh. Fresh food has a good taste, delicious taste, delicate meat, rich nutrition and health care effects, and is very popular among people. However, after the fruiting body of straw mushroom is harvested, it still carries out vigorous physiological and metabolic activities at ...

Claims

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Application Information

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IPC IPC(8): A01H15/00A01G1/04
Inventor 唐雪明祝子坪吕贝贝吴潇何建华蒋玮王金斌
Owner SHANGHAI ACAD OF AGRI SCI
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