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Rhodotorula mucilaginosa for removing total nitrogen in sewage and application of rhodotorula mucilaginosa

A technology of Rhododendron gum and sewage, which is applied in biological water/sewage treatment, water/sludge/sewage treatment, water pollutants, etc., can solve the problems of many processes, long energy consumption and time, and no separation of pure bacteria. , to achieve the effect of a wide range of applications

Active Publication Date: 2020-05-26
湖北宜水生物科技有限公司
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AI Technical Summary

Problems solved by technology

[0003] At present, domestic research on denitrification microorganisms is mainly focused on the degradation effect of mixed flora and the improvement of denitrification and denitrification process. No pure bacteria with the function of total nitrogen removal have been isolated. The denitrification needs to be started through the reactor and The domestication and cultivation of mixed flora can only be completed, with many procedures, long energy consumption and time

Method used

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  • Rhodotorula mucilaginosa for removing total nitrogen in sewage and application of rhodotorula mucilaginosa
  • Rhodotorula mucilaginosa for removing total nitrogen in sewage and application of rhodotorula mucilaginosa
  • Rhodotorula mucilaginosa for removing total nitrogen in sewage and application of rhodotorula mucilaginosa

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Experimental program
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Effect test

specific Embodiment 1

[0016] Isolation Source of Rhodotorula colloides NBUY-9 Strain

[0017] 1. Weigh 10 g of activated sludge from the aerobic pool of a sewage treatment station in a fish processing factory in Ningbo, inoculate it into a triangular flask containing 90 m1 of YPD liquid medium, and shake it at 30°C and 150 r / min. bottle culture, adding 5-15 ml of 10% ammonia water to the culture medium every 24 hours to enrich the nitrogen-tolerant yeasts. After 4 days of culture, take the liquid culture medium and add it to another A fresh YPD liquid culture medium, repeated 3 times, and co-cultured for 12 days to obtain a yeast culture medium; wherein the YPD liquid medium composition (g / L): yeast powder 10.0, peptone 20, glucose 20, pH value 6.5, distilled water 1 L.

[0018] 2. Dilute the obtained yeast culture solution and spread it on a YPD solid medium plate, culture it at 30°C for 3 days, pick a single colony for streaking, separation and purification, and repeat twice to obtain pure bacte...

specific Embodiment 2

[0025] Cultivation of Rhodotorula gluei NBUY-9 cells: take the 2nd round collection number of CCTCC NO: M 20191015 Rhodotorula mucilaginosa ) NBUY-9 was inoculated in 100 mL of YPD liquid medium, cultured with shaking at 28 °C and 180 r / min for 24 h to obtain yeast cell liquid, and 12 g of wet cells were taken as the experimental group, which was used in the following sewage treatment ;

[0026] Another strain of yeast preserved in this laboratory, Candida tropicalis ( Candida tropicalis ) ZJY-7 was used as the experimental control group 1. Carry out cell culture in the same way, take 2 rings of Candida tropicalis ( Candida tropicalis ) ZJY-7 strain was inoculated in 100 mL of YPD liquid medium, and cultured with shaking at 28 °C and 180 r / min for 24 h to obtain yeast cell liquid, and 12 g of wet cells were taken as control group 1 for the following sewage treatment.

[0027] Take a certain amount of activated sludge from a municipal domestic sewage treatment plant, and ...

Embodiment 1

[0029] Application of Rhodotorula colloides NBUY-9 to Degrade Ammonia Nitrogen, Total Nitrogen and COD in Artificial Wastewater Containing Inorganic Nitrogen

[0030]Artificial sewage: prepare 3 L sewage with glucose and ammonium chloride according to C:N=100:5, and add a small amount of K 2 HPO 4 , adjust the pH to 6.5~8.0, and the prepared artificial sewage contains ammonia nitrogen, TN, and COD concentrations of about 40 mg / L, 40 mg / L, and 200 mg / L, respectively. The samples of the experimental group, control group 1 and control group 2 were added to the same circular reactor, and 1 L of artificial sewage was added to the three reactors respectively. The reactor is equipped with an aeration device, and the dissolved oxygen is about 4 mg / L. The top of the reactor is sealed with a sterile sealing film to prevent the entry of bacteria. Then carry out aeration treatment. Samples were taken every 1 h, and the pH value was determined to be around 6.5-8.0, which was within the...

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Abstract

The invention discloses rhodotorula mucilaginosa for removing total nitrogen in sewage and application of the rhodotorula mucilaginosa. The rhodotorula mucilaginosa NBUY-9 is is preserved in China Center for Type Culture Collection on December 5, 2019, the preservation number is CCTCC NO:M 20191015, the rhodotorula mucilaginosa grows on a YPD flat plate without seawater salinity, the bacterial colony morphology characteristic is orange red, the surface is round, smooth, wet and opaque, the strain can grow in a relatively wide pH range of 2.5 to 9.0, and the optimal growth temperature is 20-30DEG C. The rhodotorula mucilaginosa can be applied to preparation of a total nitrogen removal agent, an ammonia nitrogen removal agent and a COD removal agent in domestic sewage and preparation of anammonia gas degradation agent in industrial waste gas, and has the advantages that ammonia nitrogen, total nitrogen and COD in the sewage can be efficiently removed at the same time, and ammonia gas components in the industrial waste gas can be efficiently degraded.

Description

technical field [0001] The invention belongs to the technical field of sewage treatment, and in particular relates to a rhodotorula japonicus capable of simultaneously removing ammonia nitrogen and total nitrogen from sewage and an application thereof. Background technique [0002] Rapid economic development has led to larger urbanization scales, increasing human activities, and increasing domestic sewage discharges. The eutrophication of water bodies caused by excessive nitrogen concentrations in water bodies has become a global water pollution problem. In 2002, my country newly formulated the comprehensive urban sewage discharge standard (GB19818-2002), which raised the standard for urban domestic sewage treatment and discharge. The first-level A standard for the maximum allowable discharge concentration (daily average mg / L) is: ammonia nitrogen (NH3 -N) ≤ 5; total nitrogen (TN) ≤ 15; COD Cr ≤50. In recent years, many provinces and cities in China have formulated more str...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34B01D53/84C12N1/16C12R1/645C02F101/16C02F101/30
CPCC02F3/347B01D53/84C02F2101/16C02F2101/30C02F2209/08C02F2209/14C02F2209/16B01D2257/406C12R2001/645C12N1/145Y02A50/20
Inventor 庄荣玉吕文洲徐兰刘军钱冬吴振李美燕潜丽珍任雨婷周松王如晨杨文鸽
Owner 湖北宜水生物科技有限公司
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