Achromobacter strain with monomethylamine degradation ability and application thereof

A technology of Achromobacter and monomethylamine, applied in the field of microorganisms, can solve the problem that no Achromobacter degrades monomethylamine, etc.

Active Publication Date: 2018-04-13
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there have been no relevant reports and patents on the use of Achromobacter to degrade monomethylamine

Method used

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  • Achromobacter strain with monomethylamine degradation ability and application thereof
  • Achromobacter strain with monomethylamine degradation ability and application thereof
  • Achromobacter strain with monomethylamine degradation ability and application thereof

Examples

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Embodiment 1

[0025] A colorless bacterium with the ability to degrade monomethylamine. The name of the strain is Achromobacter xylosoxidans GDUTAN5. It is preserved in the China Center for Type Culture Collection at the campus of Wuhan University in Wuhan City, Hubei Province; the preservation number is CCTCC NO: M 2017285, the date of deposit is May 24, 2017.

[0026] The Achromobacter GDUTAN5 of this example was isolated and screened from a landfill leachate in Guangzhou City, Guangdong Province. Its separation and purification method is as follows: the acclimatization culture fluid used is the aqueous solution of inorganic salt cultivation (contain in every 1000mL aqueous solution of inorganic salt cultivation: K 2 HPO 4 ·3H 2 O 1.2g, KH 2 PO 4 1.2g, NH 4 Cl 0.4g, MgSO 4 ·7H 2 O 0.2g, FeSO 4 ·7H 2 O0.01g and 1mL trace element aqueous solution. Among them, every 1000mL trace element aqueous solution contains: CaCl 2 2H 2 O 0.2g, MnSO 4 4H 2 O 0.2g, CuSO 4 2H 2 O0.01g, ZnS...

Embodiment 2

[0045] This example is the application of Achromobacter xylosoxidans GDUTAN5 in environmental restoration, which can degrade monomethylamine in the environment. The environment includes the atmosphere, water body or soil.

[0046] The monomethylamine degradation ability of the Achromobacter xylosoxidans (Achromobacterxylosoxidans) GDUTAN5 obtained by the screening of the present invention is tested below:

[0047] According to the needs of the degradation experiment, configure inorganic salt medium: add 100 mL of inorganic salt solution to the 300 mL serum bottle (each 100 mL of inorganic salt solution contains: K 2 HPO 4 ·3H 2 O 0.12g, KH 2 PO 4 0.12g, NH 4 Cl 0.04g, MgSO 4 ·7H 2 O 0.02g, FeSO 4 ·7H 2 O 0.001g, CaCl 2 2H 2 O 0.02g, MnSO 4 4H 2 O 0.02g, CuSO 4 2H 2 O0.001g, ZnSO 4 ·7H 2 O 0.02g, CoCl 2 ·6H 2 O 0.009g, Na 2 MoO 4 2H 2 O 0.012g, H 3 BO 3 0.0006g, double distilled water 100mL), autoclaved at 121℃ for 30min. Firstly, the Achromobacter GDUT...

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Abstract

The invention discloses an achromobacter strain with monomethylamine degradation ability and application thereof. The name of the strain is Achromobacter xylosoxidans GDUTAN5 which is preserved in theChina Center for Type Culture Collection at Wuhan University, No. 299, Bayi Road, Wuchang District, Wuhan, Hubei Province, China. The preservation number is CCTCC NO:M 2017285. The preservation dateis May 24, 2017. The Achromobacter xylosoxidans GDUTAN5 is Gram-negative and rod-shaped, has a round colony morphology, is light yellow and opaque, and has a smooth surface and a colony diameter of 1-2 mm. The Achromobacter xylosoxidans GDUTAN5 can be applied to environmental remediation for degradation of monomethylamine in the environment and has a high degradation rate. When the substrate concentration is 5 mg / L, the degradation rate can reach 92.3% after 96 h of degradation.

Description

technical field [0001] The invention belongs to the technical field of microbes, and more specifically relates to an Achromobacter strain capable of degrading monomethylamine and its application. Background technique [0002] Methylamine organic compounds such as methylamine (Methylamine or methylamine), dimethylamine (dimethylamine) and trimethylamine (trimethylamine) are a class of main products used in the production of dyes, rubber, pesticides, pharmaceuticals, cosmetics and corrosion inhibitors and other pharmaceutical or chemical industries. raw materials or intermediate products. Not only do they have a strong, foul odor, but they can cause severe irritation of the skin, eyes, nose, throat or respiratory system when humans are exposed to them. Among them, methylamine is the simplest methylamine compound, which is widely used in industry, but due to its low boiling point and high vapor pressure, it will volatilize in large quantities into the environment during produc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10A62D3/02C12R1/025C02F101/38A62D101/26
CPCA62D3/02C02F3/34B09C1/10C02F2101/38A62D2101/26C12R2001/025C12N1/205
Inventor 李桂英李世蔼安太成刘宏利陈江耀
Owner GUANGDONG UNIV OF TECH
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