Separation and identification of Lysobacter gummosus OH17 with wide spectrum and high efficiency antagonistic action on plant pathogenic fungi and oomycete
A technology of phytopathogenic fungus, Lysobacter colloidus, applied in the field of microbiology
Inactive Publication Date: 2015-12-02
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology
Chemical pesticides are generally used to prevent and control fungal diseases in production. However, food quality and safety issues and ecological security issues caused by chemical pesticide residues have become the focus of attention from all walks of life.
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[0013] The present invention will be further elaborated below through specific implementation, but the present invention is not limited.
[0014] 1 Materials and methods
[0015] 1.1 Test strains, medium
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Abstract
The invention relates to a biocontrol bacterium strain OH17 with good antagonistic activity for plant pathogenic fungi and oomycete. The bacterial strain is characterized in that: the bacterial strain is Lysobacter gummosus, which belongs to Lysobacter of Xanthomonadaceae, with CGMCC No. 8649. The form of the bacterium colony is as follows: the bacterium colony presents different forms on different mediums, for example, the bacterium colony is in light yellow color on a NA medium, the thallus surface is smooth with luster, and the surface is protruding; the bacterium colony is always in yellow color on an LB medium with slow growth; the bacterium colony is white on a 10% TSA medium with flat surface; the bacterium colony does not grow on any TSA medium. The bacterium is gram-negative and in a rod shape without any flagellum. The optimum growth temperature is 28 DEG C. The bacterial strain has a wide spectrum and high efficiency antagonistic action on plant pathogenic fungi and oomycete.
Description
(1) Technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to the isolation and identification of a bacterial strain with antagonistic activity to plant pathogenic fungi and oomycetes. (2) Background technology [0002] Diseases caused by pathogenic fungi account for about 70-80% of plant diseases and are one of the main obstacles affecting agricultural output, quality and benefit in our country. Chemical pesticides are generally used to prevent and control fungal diseases in production. However, food quality and safety issues and ecological security issues caused by chemical pesticide residues have become the focus of attention from all walks of life. [0003] The development of ecologically safe and environmentally friendly biopesticides is one of the effective ways to solve the above problems. The biopesticides currently used are mainly microbial pesticides and agricultural antibiotics. The former mainly use living microorg...
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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01P3/00C12R1/01
Inventor 刘凤权钱国良刘琳琳沈艳
Owner NANJING AGRICULTURAL UNIVERSITY
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