Method for preparing culture medium for industrial fermentation by using agricultural raw materials subjected to steam explosion treatment
A medium and raw material technology, applied in the field of effective preparation of industrial fermentation medium from agricultural raw materials, can solve the problems of high resource and energy consumption, large consumption of chemical reagents, loss of nutrients, etc., and reduce energy consumption and consumption of chemical reagents , Improve the utilization rate of raw materials, improve the effect of porosity
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Embodiment 1
[0024] Take 65 g of soybean meal and 110 g of bran, crush them to 10 mesh, adjust the water content to 30%, put them in a 4 L steam explosion equipment, pass in saturated water steam, maintain 5 min at 0.7 MPa, release the pressure suddenly, and collect the steam Add 2 g of glucose, 6 g of whey protein, 3 g of calcium carbonate, 0.2 g of ammonium sulfate, and 0.1 g of magnesium sulfate to the material after blasting, add appropriate amount of distilled water to a water content of 43%, and put the evenly stirred culture medium into stainless steel In the iron basin, carry out steam sterilization, and insert 4×10 6 cfu / g Lactobacillus casei , placed in a constant temperature incubator, and at the same time, the traditional culture medium was used as a control, and the culture medium was cultured for 60 hours. The culture medium treated by steam explosion, after the fermentation, the number of bacteria in the fermented product was 3.57×1010 10 cfu / g, the number of live bacter...
Embodiment 2
[0026] Take 105 g of corn stalk powder and 85 g of bran, grind them to 40 mesh, adjust the water content to 30%, put them in a 4 L steam explosion equipment, inject saturated water vapor, maintain 5 min at 1.2 MPa, and suddenly release the pressure. Collect the material after steam explosion, add 10g potassium dihydrogen sulfate, 20g ammonium sulfate, 2g calcium chloride, 15g magnesium sulfate heptahydrate, 0.02g ferrous sulfate heptahydrate, 0.032g manganese sulfate, 0.034g zinc chloride, 0.04 g cobalt chloride, add an appropriate amount of distilled water to a water content of 43%, put the evenly stirred culture medium into a stainless steel iron basin, carry out steam sterilization, and insert 4×10 6 cfu / g Trichoderm a viride , placed in a constant temperature incubator, and at the same time compared with the traditional medium production method, cultured for 120 hours, after the fermentation, the medium treated by steam explosion, the CMC enzyme activity reached 1100 IU / ...
Embodiment 3
[0028] Take 500 g of corn slag, adjust the water content to 50%, put it in a 4 L steam explosion equipment, pass in saturated water steam, maintain it at 0.5 MPa for 10 min, release the pressure suddenly, collect the material after steam explosion, dry it and crush it to 100 mesh, 6% corn mash medium was prepared, and at the same time, the corn that had not been steam-exploded and crushed was used as a raw material to prepare the medium as a control. After the corn mash medium was sterilized, inoculate Clostridium acetobutylicum ATCC 824, the inoculum size was 10% (volume ratio), and the solvent content in the fermentation medium was determined after anaerobic culture for 72 h. The solvent yield of untreated corn mash medium was 2.285%, including 10.69 g / l butanol, 2.48 g / l acetone and 0.54 g / l ethanol. The solvent yield of corn mash medium after steam explosion was 2.56%, among which butanol was 12.288 g / l, acetone was 2.304 g / l, and ethanol was 0.768 g / l. The total outpu...
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