Method for preparing culture medium for industrial fermentation by using agricultural raw materials subjected to steam explosion treatment

A medium and raw material technology, applied in the field of effective preparation of industrial fermentation medium from agricultural raw materials, can solve the problems of high resource and energy consumption, large consumption of chemical reagents, loss of nutrients, etc., and reduce energy consumption and consumption of chemical reagents , Improve the utilization rate of raw materials, improve the effect of porosity

Inactive Publication Date: 2014-06-25
北京中科百瑞能工程技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of low utilization rate of raw materials, large consumption of chemical reagents, serious loss of nutrients, ineffective utilization of cell wall substances, and high consumption of resources and energy in agricultural raw materials used as fermentation medium in my country, a method to effectively improve the utilization of raw materials is proposed. The preparation method of the culture medium for the fermentation industry that improves the product quality while improving the efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take 65 g of soybean meal and 110 g of bran, crush them to 10 mesh, adjust the water content to 30%, put them in a 4 L steam explosion equipment, pass in saturated water steam, maintain 5 min at 0.7 MPa, release the pressure suddenly, and collect the steam Add 2 g of glucose, 6 g of whey protein, 3 g of calcium carbonate, 0.2 g of ammonium sulfate, and 0.1 g of magnesium sulfate to the material after blasting, add appropriate amount of distilled water to a water content of 43%, and put the evenly stirred culture medium into stainless steel In the iron basin, carry out steam sterilization, and insert 4×10 6 cfu / g Lactobacillus casei , placed in a constant temperature incubator, and at the same time, the traditional culture medium was used as a control, and the culture medium was cultured for 60 hours. The culture medium treated by steam explosion, after the fermentation, the number of bacteria in the fermented product was 3.57×1010 10 cfu / g, the number of live bacter...

Embodiment 2

[0026] Take 105 g of corn stalk powder and 85 g of bran, grind them to 40 mesh, adjust the water content to 30%, put them in a 4 L steam explosion equipment, inject saturated water vapor, maintain 5 min at 1.2 MPa, and suddenly release the pressure. Collect the material after steam explosion, add 10g potassium dihydrogen sulfate, 20g ammonium sulfate, 2g calcium chloride, 15g magnesium sulfate heptahydrate, 0.02g ferrous sulfate heptahydrate, 0.032g manganese sulfate, 0.034g zinc chloride, 0.04 g cobalt chloride, add an appropriate amount of distilled water to a water content of 43%, put the evenly stirred culture medium into a stainless steel iron basin, carry out steam sterilization, and insert 4×10 6 cfu / g Trichoderm a viride , placed in a constant temperature incubator, and at the same time compared with the traditional medium production method, cultured for 120 hours, after the fermentation, the medium treated by steam explosion, the CMC enzyme activity reached 1100 IU / ...

Embodiment 3

[0028] Take 500 g of corn slag, adjust the water content to 50%, put it in a 4 L steam explosion equipment, pass in saturated water steam, maintain it at 0.5 MPa for 10 min, release the pressure suddenly, collect the material after steam explosion, dry it and crush it to 100 mesh, 6% corn mash medium was prepared, and at the same time, the corn that had not been steam-exploded and crushed was used as a raw material to prepare the medium as a control. After the corn mash medium was sterilized, inoculate Clostridium acetobutylicum ATCC 824, the inoculum size was 10% (volume ratio), and the solvent content in the fermentation medium was determined after anaerobic culture for 72 h. The solvent yield of untreated corn mash medium was 2.285%, including 10.69 g / l butanol, 2.48 g / l acetone and 0.54 g / l ethanol. The solvent yield of corn mash medium after steam explosion was 2.56%, among which butanol was 12.288 g / l, acetone was 2.304 g / l, and ethanol was 0.768 g / l. The total outpu...

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Abstract

The invention discloses a method for processing agricultural raw materials and producing a culture medium for industrial fermentation by utilizing a steam explosion technology aiming at the problems that the raw material utilization rate is low, the crushing power consumption is high, the amount of hydrolysis acids and alkalis is large and the like when the agricultural raw materials are used for an industrial fermentation culture medium. By utilizing the steam explosion technology, starch, proteins, lipids, celluloses, hemicelluloses, lignins and other components in the agricultural raw materials are degraded or modified, macromolecular substances are degraded into small molecular substances, and fermentation promoted substances are generated. According to the method, the energy consumption in the process of further processing the agricultural raw materials is reduced, the product performance of the agricultural raw materials serving as the industrial fermentation culture medium is improved, the raw material utilization rate in the fermentation process is improved, and growth and metabolism of microbes and the conversion rate of the fermented product are promoted by a fermentation accelerant generated in the steam explosion treatment process. The agricultural raw materials subjected to the steam explosion treatment can serve as a culture medium for solid state fermentation and also can be used for a culture medium for liquid state fermentation.

Description

technical field [0001] The invention belongs to the field of production and processing of industrial fermentation medium, in particular to a method for effectively preparing industrial fermentation medium from agricultural raw materials. Background technique [0002] Fermentation industry plays an important role in my country's national economy and social development, and the raw materials for fermentation medium are mainly agricultural raw materials. Agricultural raw materials include agricultural products, agricultural product processing residues, agricultural residues, etc. Among them, agricultural products mainly include corn, wheat, rice, miscellaneous grains, soybeans, etc.; agricultural product processing residues mainly include soybean meal, peanut meal, bran, etc.; agricultural residues mainly include crop straw, etc. Among them, the proportion of non-grain raw materials is about 10%. Taking corn as an example, the corn used in the fermentation industry in my count...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/42C12N9/24C12P7/16C12P7/36C12P7/06C12R1/245C12R1/885C12R1/145
CPCY02E50/10
Inventor 魏远
Owner 北京中科百瑞能工程技术有限责任公司
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