71 results about "Organism Strain" patented technology

Platforms, systems, and methods for genetic generalization in synthetic biology development. According to one aspect, there is provided a method for predicting performance associated with genetic edits, the method comprising: receiving, by a platform, information about a strain of a microorganism, wherein the information about the strain comprises information describing a plurality of genetic edits to a base strain of the microorganism; generating, by the platform, a set of genetic embeddings based on the information about the strain, wherein the generating comprises processing the information about the strain using one or more embedding models, wherein each of the one or more embedding models: receives the information about the strain of the microorganism as input; and applies computational transformations to the input using a corresponding embedding model to generate a multi-dimensional vector representation for each of the plurality of genetic edits.

The invention belongs to the technical field of microbial genetic engineering, and particularly relates to an alpha-2, 3-sialyltransferase mutant and application thereof. The a-2, 3-sialyltransferase mutants V175N, P198A, G173D, D174Q, F196K and A315H are obtained by performing site-specific mutagenesis on six amino acids in an a-2, 3-sialyltransferase amino acid sequence coded by a gene derived from Campylobacter jejuni (OH4384), the yield of 3 '-sialyllactose is remarkably increased by single-point mutagenesis of the V175N, the yield of 3'-sialyllactose is remarkably increased by single-point mutagenesis of the V175N, the yield of 3 '-sialyllactose is remarkably increased by single-point mutagenesis of the P198A, the G173D, the D174Q, the F196K and the A315H, the optimal recombinant microbial strain 3 '-SL-09 (V175N / P198A / D174Q) is obtained through combination of multiple mutation sites, the yield can reach 5.91 g / L, the bottleneck problem of low catalytic efficiency of sialyltransferase is effectively solved, and an enzyme element with excellent performance is provided for high-efficiency and low-cost biological manufacturing of 3'-SL.

The invention belongs to the field of membrane pollution control, and particularly relates to pseudomonas as well as a separation method and application thereof. The invention provides a pseudomonas, which is identified as Pseudomonas knackmussii HITSZ-Q1, is preserved in the Guangdong Microbiological Culture Collection Center, and has the preservation number of GDMCC (China General Microbiological Culture Collection Center) NO: 66162. The strain takes C6-HSL as the unique carbon source and nitrogen source, and the 16S rDNA sequence of the strain is as shown in SEQ ID NO. 1. The strain has a remarkable degradation capability on signal molecules such as C6-HSL, C8-HSL, 3-oxo-C12-HSL and the like, and the highest quenching rate can reach 98.9%. The strain and pseudomonas aeruginosa are co-cultured, so that the signal molecule concentration of the pseudomonas aeruginosa can be effectively reduced, and biofilm formation is inhibited. The strain is further prepared into a carrier in the form of immobilized pellets, the carrier is added into a membrane bioreactor, the operation cycle is prolonged from 6.5 days to 10.5 days, and the membrane pollution delay rate reaches up to 61.5%.

The present invention belongs to the field of resources and environment, and discloses a method for promoting chalcopyrite bioleaching by in-situ depassivation: in an anaerobic environment, passivated chalcopyrite and reduced sulfur are added to a 9K culture medium, an acidophilic microbial strain is inoculated for culture, and the chalcopyrite is collected by centrifugation; the collected chalcopyrite is dried and added to a 9K culture medium, and the acidophilic microbial strain is inoculated for culture and leaching under an aerobic environment. The present invention introduces acidophilic microorganisms and sulfur into the anaerobic system, so that the dense passivation film of the chalcopyrite can be reduced and removed, allowing the copper ions in the mineral to be leached under aerobic conditions in the later stage. This not only overcomes the shortcomings of pure chemical reaction leaching, such as high energy consumption, high consumables, and environmental pollution, but also solves the problem of low utilization efficiency due to the passivation film hindering leaching during the bioleaching process, so that the chalcopyrite can be utilized to the greatest extent. This in-situ depassivation bioleaching technology has a wide range of applications, low site requirements, and can be used on a large scale industrially.

The invention relates to the technical field of microbial metabolism, in particular to a biological reagent production optimization method and system based on microbial metabolism characteristics. The method comprises the following steps: acquiring a biological reagent to produce a microbial strain, and determining the component proportion of a microbial culture medium and optimal microbial culture conditions; carrying out microorganism culture metabolism recording and metabolic flow path analysis on the biological reagent production microorganism strain, and simultaneously carrying out metabolic characteristic analysis to obtain metabolic quantitative characteristics of the biological reagent production microorganism; carrying out metabolic bottleneck node identification analysis on the metabolic flow path of the biological reagent production microorganisms to obtain metabolic bottleneck nodes of the biological reagent production microorganisms; and carrying out microbial production optimization on the corresponding biological reagent production microbial culture metabolism process, and carrying out production post-treatment and purification separation to obtain a biological reagent production optimization product. The production efficiency of the biological reagent can be remarkably improved, so that the stability and the consistency of the biological reagent product are ensured.

The invention belongs to the technical field of microbial genetic engineering, and particularly relates to an alpha-2, 6-sialyltransferase mutant and application thereof. The a-2, 6-sialyltransferase mutants M133L, E140D, D228H, N289D and G322Q are obtained by carrying out site-specific mutagenesis on five amino acids in an a-2, 6-sialyltransferase amino acid sequence coded by a gene derived from Photobacterium sp.JT-ISH-224, and compared with wild type alpha-2, 6-sialyltransferase, the yield of the single-point mutant 6 '-SL of the E140D and the yield of the single-point mutant 6'-SL of the G322Q are increased by 87.80% and 78.01% respectively. The yield of the optimal recombinant microbial strain 6 '-SL-7 (E140D / G322Q) obtained by mutation site combination can reach 1.471 g / L, the bottleneck problem of low catalytic efficiency of sialyltransferase is effectively solved, and an enzyme element with excellent performance is provided for high-efficiency and low-cost biological manufacturing of 6'-SL.

The invention belongs to the technical field of microorganisms and aquaculture, and discloses a high-environmental-adaptability nitrification and denitrification bacillus cereus strain, a microbial agent and application of the high-environmental-adaptability nitrification and denitrification bacillus cereus strain, the high-environmental-adaptability nitrification and denitrification bacillus cereus strain is bacillus cereus HCCN1 and is preserved in Guangdong Microbial Culture Collection Center on November 13, 2024, and the preservation number is GDMCC No: 65477. The invention also discloses a microbial agent containing the strain HCCN1 as an active component and application of the microbial agent. The strain HCCN1 has high stress resistance and high environmental adaptability, has heterotrophic nitrification and aerobic denitrification functions at the same time, can efficiently degrade nitrogen and phosphorus under the severe environmental conditions of wide pH value range, temperature range, high salt and alkali concentration and the like, performs purification treatment on aquaculture water, and is high in biological safety and low in cost. The industrial requirements of efficient, healthy and sustainable aquaculture can be met.

The invention discloses a strain of Bacteroides ovatus L2 and application of the strain of Bacteroides ovatus L2 in a product for preventing and treating vomitoxin toxicity. The strain is preserved in Guangdong Microbiological Culture Collection Center, the preservation number is GDMCC.No: 67205, and the preservation date is November 4, 2025. The strain is separated and purified from a healthy adult excrement sample, has the characteristics of good acid resistance, cholate resistance, gastrointestinal fluid resistance and the like, and is free of hemolysis and good in safety. The strain can effectively relieve the toxic effect induced by vomitoxin, mainly inhibits inflammatory factors and promotes expression of intestinal barrier functional proteins, can be applied to products of microecologics for preventing and treating DON toxicity, and has a good application prospect.

The present invention provides a strain of Penicillium oxalicum that can degrade fumonisins and its application, which belongs to the technical field of biological strains. The Penicillium oxalicum strain is named FJ284, with a deposit number of CGMCC No.41242. It was deposited in the General Microbiological Center of China Microbiological Culture Collection Administration Committee on May 27, 2024, and the deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. Preferably, the Penicillium oxalicum strain is isolated from corn stalks. Application of the Penicillium oxalicum strain, the Penicillium oxalicum strain is resistant to Fusarium pseudo-verticillium. The Penicillium oxalicum strain of the present invention can inhibit the growth of plant endophytes of Fusarium pseudo-verticillium to reduce the production of corn pathogens, and at the same time reduce the production of mycotoxins to improve the quality of corn, using plant and microbial improvement in biological improvement.

This invention relates to a method for efficiently spraying soil conditioner to treat saline-alkali land using composite microorganisms, belonging to the field of soil improvement technology. The method involves screening salt-tolerant functional microbial strains to prepare a composite microbial agent, which is then mixed with carrier materials such as diatomaceous earth, soybean meal, yeast metabolites, and well-rotted farmyard manure in a specific ratio to form a conditioner for application. The conditioner is applied using a two-stage spraying method, combined with deep tillage pretreatment and controlled temperature and humidity conditions, significantly improving microbial activity and soil condition uniformity. Experimental data show that after applying this method, the soil pH value decreased to 7.3-7.5, the salt content decreased to 0.1%-0.2%, and the organic matter content increased to 18-22 g / kg, achieving efficient ecological restoration and sustainable utilization of saline-alkali land.

The invention belongs to the technical field of microorganisms and aquaculture, and discloses a Bacillus cereus strain with high environmental adaptability for nitrification and denitrification, a microbial agent and its application. Bacillus cereus HCCN1 was deposited in the Guangdong Provincial Microbial Strain Collection Center on November 13, 2024, with a deposit number of GDMCC No: 65477. The present invention also discloses a microbial agent comprising the strain HCCN1 as an active ingredient and its application. The strain HCCN1 of the present invention has high stress resistance and high environmental adaptability, and has heterotrophic nitrification and aerobic denitrification functions. It can efficiently degrade nitrogen and phosphorus under harsh environmental conditions such as a wide pH range, temperature range, and high salt and alkali concentrations, and purify aquaculture water bodies. It has high biosafety and can meet the industry needs of efficient, healthy and sustainable aquaculture.

This application relates to the field of microbial strain technology, specifically disclosing a Bacillus thuringiensis HP-SYJ2 strain and its applications. The Bacillus thuringiensis HP-SYJ2 strain is deposited at the China Center for Type Culture Collection (CCTCC), accession number: CCTCC NO: M2025343, deposit date: March 3, 2025, address: Wuhan University, Wuhan, China. The strain of this application can be used to prepare insecticides for controlling underground pests, which is of great significance for the biological control of plant diseases and pests.

The application relates to the technical field of microbial strain preservation, in particular to a microbial strain preservation device which comprises a freezer, a moving assembly and a rotating assembly, the freezer is provided with a box door, a fixing seat is arranged in the freezer, the end of the fixing seat is provided with a supporting disc, a plurality of groups of test tube racks are fixedly connected to the supporting disc, and the bottom end of the supporting plate is fixedly connected with a connecting shaft; the moving assembly is arranged in the freezer and is used for driving the supporting disc to displace; and the rotating assembly is used for driving the connecting shaft to rotate, so that the supporting disc rotates. The moving assembly and the rotating assembly are arranged, the connecting shaft drives the supporting plate to move a small distance through the action of the moving assembly, part of the position of the supporting disc is moved to the outside of the freezer, then the connecting shaft drives the supporting disc to rotate through the cooperation of the rotating assembly, and then the strain at any position on the supporting plate can be taken and placed, so that the operation is more convenient.

Genetically engineered yeast cells having disruption of pex3, pex5 or pex20 genes and combinations thereof having reduced beta-oxidation and methods for increasing polyunsaturated fatty acids are disclosed.

The present invention discloses the application of Bacillus amyloliquefaciens and its bacterial agent in the treatment of livestock and poultry feces with black soldier flies, and relates to the field of biological strain utilization and development technology. The Bacillus amyloliquefaciens LYM152 has been deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration Committee on September 17, 2019, and is classified and named Bacillus amyloliquefaciens LYM152, with a deposit number of CGMCC No.18497, and the deposit address is the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. The Bacillus amyloliquefaciens disclosed in the present invention has the characteristics of antibacterial, deodorizing, and high digestive enzyme activity. Its bacterial agent has a good deodorizing effect in the treatment of livestock and poultry feces, and the effect of strengthening the degradation of livestock and poultry feces in combination with black soldier flies is more significant.

: The present application discloses methods and compositions for enhancing antibiotic efficacy and treating microbial infections through the use of polyamine analog compounds. These compounds function as antimicrobial agents and antibiotic adjuvants, sensitizing bacteria to antibiotics by disrupting bacterial membrane integrity, inhibiting polyamine detoxification enzyme activity, and mitigating resistance mechanisms. The disclosed methods include administering to a subject an effective amount of an antibiotic in combination with one or more polyamine analog compounds of Formula I, II, or III, or their pharmaceutically acceptable salts or solvates. The compositions are effective against a wide range of Gram-positive and Gram-negative bacteria, including drug-resistant strains such as methicillin-resistant Staphylococcus aureus (MRSA) and Klebsiella pneumoniae, and fungi such as Candida albicans. The application also describes pharmaceutical formulations, dosing strategies, and synergistic effects achieved with these compounds, addressing the urgent need for innovative solutions to combat antimicrobial resistance and improve the treatment of severe bacterial infections. The compositions are designed for use in human and veterinary applications, providing effective solutions for managing resistant microbial strains and managing disorders related to polyamine detoxification enzymes in human and animals.

The invention relates to a recombinant microbial strain for de novo synthesis of gardenin B and application of the recombinant microbial strain in production of polymethoxylated flavone compounds, and belongs to a cross technology of synthetic biology and organic synthetic chemistry. The invention designs and constructs a heterozygous gardenin B biosynthesis pathway, and obtains a microbial strain for synthesizing gardenin B from the beginning. By screening F8H and redox partner types and regulating and controlling subcellular localization, an F8H-redox partner combination with optimal space and type is determined, and an engineering strain of which the gardenin B yield is greatly improved is obtained. The efficient synthesis method of the polymethoxylated flavonoids is established by selecting recombinant microorganisms for synthesizing gardenin B from de novo to perform co-culture fermentation or chemical conversion of fermentation products.

The invention discloses a method for degrading vomitoxin in coconut meal through microbial fermentation, and belongs to the technical field of coconut meal fermentation. In order to solve the problems of limitation of microbial strain screening culture, insufficient vomitoxin degradation efficiency and inaccurate fermentation process control, by screening and domesticating microbial strains, the specific enzyme activity is remarkably improved, efficient degradation of vomitoxin is realized, an environment suitable for microbial growth and metabolism is provided, and the vomitoxin degradation efficiency is improved. By combining the synergistic effect of microbial fermentation and an exogenous enzyme preparation, the vomitoxin degradation efficiency is improved to the greatest extent, the limitation of single microbial metabolism is broken through, particularly, remarkable advantages are shown in a high-mycin-polluted complex matrix, the vomitoxin content is remarkably reduced, and the vomitoxin degradation efficiency is improved to the greatest extent. The substrate quality of the coconut meal is maintained, and the efficient, safe and controllable vomitoxin degradation method is constructed through optimization treatment of the coconut meal substrate, microbial screening and domestication and the synergistic effect of exogenous enzymes.

The invention relates to a microbial strain DNA (deoxyribonucleic acid) mixed coding and an alternating Transformer depth prediction method for gene expression of the microbial strain DNA mixed coding, and belongs to the crossing field of bioinformatics and artificial intelligence. The core of the method is to construct a deep learning model capable of efficiently capturing DNA sequence characteristics, and end-to-end accurate prediction of the expression level of a target gene in a specific microbial host is realized. The method comprises the following steps: carrying out binary coding on four conventional basic groups of DNA of a target strain, splicing eight key biological characteristics closely related to gene expression, and carrying out hybrid coding to generate a combined characteristic matrix; a deep learning network containing an alternating Transform coding structure is established, local structure information of a DNA combination feature matrix and a global context dependency relationship are deeply fused, and the analysis precision of microorganism DNA sequence data and the prediction accuracy of a gene expression level are improved. According to the technical scheme, an efficient and accurate computer-aided prediction tool is provided for optimization design of genetic elements (such as promoters) and gene sequences, the expensive experiment trial and error cost and the strain characterization cost can be remarkably reduced, and the research and development process of high-yield strains is accelerated.

The present invention belongs to the field of microbial technology, and particularly relates to a phosphate-solubilizing Aspergillus terreus and its application in drought-resistant probiotics; the Aspergillus terreus of the present invention is obtained by screening the rhizosphere soil of pepper, and the Aspergillus terreus CY-05 ( Aspergillus terreus var. fluffy ) is a growth-promoting microbial strain with disease resistance, high yield of terreus ketone and phosphate solubilization ability, and is an excellent strain with strong survival ability and multiple functions such as improving crop drought tolerance. The present invention also provides a probiotic agent obtained by solid-state fermentation of Aspergillus terreus CY-05. The application of this agent can increase the tolerance of crops to drought, improve the yield and survival ability of crops under drought stress conditions, promote the conversion and utilization of insoluble phosphorus, and prevent and control the occurrence of root soil-borne diseases. Strain CY-05 is an indigenous fungus in the rhizosphere of pepper. It is non-toxic and harmless to humans and animals, does not cause environmental pollution, and helps to solve the problems of water shortage and phosphorus deficiency faced by agricultural production in large semi-arid planting areas. It has important economic significance.

To provide methods for producing and identifying Paenibacillus sp. strains with enhanced fungicidal activity and processability.SOLUTION: The present invention provides a composition comprising a biologically pure culture of a Paenibacillus sp. strain comprising a mutant DegU lacking a functional receiver domain or a functional DNA binding domain and / or a mutant DegS lacking a functional single binding domain or a functional ATPase domain with decreased viscosity in a liquid culture. Also provided is a method of identifying a Paenibacillus sp. mutant derivative strain with decreased viscosity in a liquid culture compared to a Paenibacillus sp. parental strain with a visual screen for mutant with a non-mucoid morphology.SELECTED DRAWING: Figure 1

Various embodiments of the present specification relate to a method of optimizing a combination of microbial strains and predicting the growth status of the strains. A method for identifying a microbial strain combination according to one embodiment of the present specification for achieving the above-described technical problem may comprise a step for obtaining genome analysis information relating to a target microorganism; a step of obtaining first metabolic information relating to each of a plurality of first microorganism colonies including the target microorganism; a step of estimating first growth index information relating to each of the plurality of first microbial colonies by using the genome analysis information and the metabolism information as inputs of a first model; and a step of determining a strain combination on the basis of at least one of the metabolism information and the growth index information.

The application discloses a coal gangue microbial active substrate for planting and a preparation method thereof. The application can dissociate effective mineral elements in coal gangue through the decomposition and activation of microorganisms, and can form a microbial substrate for planting by cooperating with added biogenic solid waste such as biogas residue, coconut husk, poultry manure and vinegar residue. The prepared microbial substrate contains active microbial strains which can continuously decompose and activate coal gangue, and can continuously release mineral elements necessary for plant growth, and can decompose organic matters in biogenic solid waste. Through the added biogenic solid waste, a certain particle size, loose and breathable physical structure and stable physicochemical properties are formed, and the physical structure can stably provide nutrient elements and organic matters for plants in the plant growth period. The microbial active substrate prepared by using the application can slowly provide necessary nutrient substances for plants in the cultivation period, and can better promote plant growth.

The present invention provides a terpenoid biosynthetic gene cluster, its expression cassette, expression vector, and recombinant microbial strain. The terpenoid biosynthetic gene cluster comprises at least one of a sesquiterpenoid and a diterpenoid. The terpenoid biosynthetic gene cluster comprises four gene clusters, and a plasmid library and a strain library containing functional genes from the gene clusters are obtained. The present invention ferments and tests the obtained strain library to obtain a product library including 36 terpenoid compounds. Furthermore, the present invention identifies the structures of four terpenoid compounds through product purification and NMR analysis. The terpenoid gene clusters screened by the present invention are capable of producing sesquiterpenoid and diterpenoid compounds, enriching the variety and library capacity of terpenoid natural products.

The present invention discloses a method, an identification device, and related equipment for microbial identification, and relates to the technical field of microbial strain identification. The method for microbial identification provided by the present invention has high resolution for microbial strain identification, and can accurately identify and differentiate strains with high similarity, thereby achieving accurate identification of strains at the same level during target strain screening to obtain strains with the same target function. The provision of the method of the present invention helps to shorten the development cycle and improve the accuracy and effectiveness of functional strain screening in the process of screening microbial strains for functional strains in medicine, food, animal or plant microbiomes, environmental treatment, and agriculture. In addition, the method of the present invention can also effectively identify new species at the same time.

The application discloses a kind of fluorine-resistant trichoderma asperellum and the fungicide prepared by it, belong to microbial strain and application technical field.The strain is named Trichoderma asperellum M700, has been preserved in China typical culture preservation center (CCTC), and its preservation number is CCTCC NO: M 2024579, and the preservation date is March 27, 2024.The beneficial effect: the trichoderma asperellum strain of the application Trichoderma asperellum. Colony growth early stage presents white mycelium, later generation green spore, causes the body to present yellow green, green, aerobic, can survive at ph 3.0-10.0, temperature is 25-28 DEG C.The strain has very good resistance, and can grow when the fluorine ion concentration is below 800 mg / L.Can very good dispel fluorine element in water body.

Provided are a microbial strain of Lachnospiraceae, a drug for preventing and / or treating at least one of tumors, metabolic diseases, diabetes, autoimmune diseases, infectious diseases, central nervous system diseases and inflammatory bowel diseases, and the use. The microbial strain of Lachnospiraceae is a new genus and species (Lachnospiraceae sp.) of the Lachnospiraceae, the nucleotide sequence of 16S rDNA is as shown in SEQ ID No. 1, and the microbial strain of the Lachnospiraceae can inhibit the growth rate of tumors and can be used for the prevention and / or treatment of tumors. The tumors include at least one of the following: liver cancer, colon cancer, rectal cancer, colorectal cancer, lung cancer, breast cancer, cervical cancer, ovarian cancer, pancreatic cancer, bile duct cancer, renal cancer and fibrosarcoma.

The invention belongs to the technical field of microorganisms, and particularly relates to saccharomyces boulardii BHS09, a microbial agent and application of the saccharomyces boulardii BHS09 in agriculture. The preservation number of the bacterial strain BHS09 provided by the invention is GDMCC (Graphite December for March Center) No. 66542. The strain has high phosphorus dissolving capacity and potassium dissolving capacity, and the potassium dissolving rate is 46.83%; the capacity of high-yield IAA is achieved, and the yield reaches 24 mg / L; the inhibition rates of the volatile gas to curvularia maize, fusarium moniliforme, fusarium pseudograminearum and fusarium oxysporum are respectively 11.64%, 54.82%, 46.48% and 51.80%, and the inhibition rates of the volatile gas to rhizoctonia solani, fusarium verticillium, curvularia maize, fusarium moniliforme, fusarium pseudograminearum, fusarium graminearum, phytophthora nicotianae and fusarium oxysporum are 40.05%-76.02%; the strain has an obvious growth promoting effect on wheat, and provides a strain resource for excavation of composite microbial strains with multiple functions.