135 results about "Organism Strain" patented technology

DNA constructs and genetically engineered microbial strains constructed using these DNA constructs, which produce a nuclease enzyme with specificity for DNA and / or RNA, are provided. These strains secrete nuclease into the periplasm or growth medium in an amount effective to enhance productivity and / or recovery of polymer, and are particularly suited for use in high cell density fermentation processes. These constructs are useful for modifying microbial strains to improve production and recovery processes for polymers such as intracellular proteins, such as enzymes, growth factors, and cytokines; for producing polyhydroxyalkanoates; and for producing extracellular polysaccharides, such as xanthan gum, alginates, gellan gum, zooglan, hyaluronic acid and microbial cellulose.

The invention relates to the field of microbial control, in particular to bacillusamyloliquefaciens subsp Lh-1 and application thereof. With a preservation number of CGMCC No.8548, the bacillusamyloliquefaciens subsp Lh-1 is applied in control of Verticillium dahliae, erwinia carotovora, ralstonia solanacearum, valsa leucostoma, Valsa Mali Miyabe et Yamada, Phytophthora capsici and phytophthora infestans. According to the invention, the bacillusamyloliquefaciens subsp Lh-1 and pesticide and chitosan oligosaccharide with dual effects of fertilizer and pesticide are combined into an Lh-1 microecological agent, the viable count is small, the dosage is small, the production process is simple, the cost is low, and the Lh-1 microecological agent has the dual functions of disease prevention and growth promotion. Nursery test proves that reduce the disease incidence of crop is reduced, especially for control of Verticillium dahliae, the disease incidence drops to 6%, and the control effect is significant. In addition, the Lh-1 microecological agent is a biological strain preparation, is completely free of a series of problems caused by use of chemical pesticides, thus being conducive to pollution-free production of crops, and farmers can have no need for pesticide or reduce the dosage of chemical pesticide, thus greatly saving the expenditure.

This invention relates to a environmental friendly multifunctional soil conditioner, and it prepared by mixed culture fermentation with composting product heaping and retting by vegetal organic offal, such as, dry branches and fallen leaves, traditional medicine gruffs and coconut chaff, natural mineral material (maifanshi, bentonite, zeolite), ammonium 2, 4-dinitrophenolate, organic amendment (Acid precipitation of lignin, glutamate dregs and furfurol dregs) and micro-organism strain. This soil conditioner will outstandingly improve physical and chemical characteristic of soil and promote the formation of the soil coacervate. It can also neutralize acid base nature of the soil and improve the cation exchange capacity of the soil. It can also outstandingly improve the growth and reproductive of the crop and the availability of the fertilizer. It has the merits of using reproducible material, changing waste into resources, obviously ecological advantage and economic returns.

Use of at least one strain of a microorganism and / or a metabolite thereof in the manufacture of a support for administration to a subject for modulating satiety signalling, wherein the support is a pharmaceutically acceptable support or a food product. Suitably, the at least one strain of a microorganism and / or a metabolite thereof may be administered to the subject for the treatment and / or prevention of excess weight and / or a disease caused by excess weight. Likewise, the at least one strain of a microorganism and / or a metabolite thereof is administered to the subject for the treatment and / or prevention of obesity and / or a caused by obesity. Preferably, the microorganism is a probiotic microorganism. Suitably the microorganism may be a lactic acid bacterium. Li one embodiment the microorganism is a strain of Lactobacillus spp. and / or Bifidobacterium spp., for example a strain of Lactobacillus acidophilus, L. curvatus, L. salivarius and / or B. lactis.

The invention discloses breeding of a microbial strain for producing 3-hydroxy-2-butanone by fermenting glucose serving as a substrate, and belongs to the technical field of fermentation in bioengineering. The screened strain is classified and named Baclliussubtilis JNA, which has been preserved in China center for type culturecollection with the preservation number of CCTCC M209309. The strain capable of producing 3-hydroxy-2-butanone by fermenting the substrate glucose is screened from natural soil, is identified in the aspects of morphology and genetics, and is named Baclliussubtilis JNA. The fermentation products are subjected to qualitative identification, the content of the 3-hydroxy-2-butanone in fermentation liquor is about 50g / L through the detection of gas chromatography (GC), and helpful guidance is provided for industrial production of the 3-hydroxy-2-butanone by a microbial fermentation method.

The present invention has for its object to provide a recombinant microbial strain capable of stably producing a polyhydroxyalkanoic acid (PHA) at a high production rate in an industrial fermentation process.The present invention relates to a recombinant microbial strain prepared by substituting an exogenous polyhydroxyalkanoic acid synthase gene for a polyhydroxyalkanoic acid synthase gene on the chromosome of the microorganism.

The present invention discloses a Proteus mirabilis strain and a method for producing S-equol through daidzein conversion by using the Proteus mirabilis strain, wherein the strain is preserved in the China Center for Type Culture Collection on November 13, 2011, and a preservation number is CCTCC M 2011390. In the prior art, the existing technology is difficult to prepare the S-equol through conversion. With the present invention, the problem in the prior art is solved, and a single function microorganism strain for directly converting a prochiral compound daidzein into a chiral compound S-equol and a preparation method thereof are provided; and compared with the strictly anaerobic equol conversion strains and the mixing bacteria conversion in the previous reports, the Proteus mirabilis LH-52 strain of the present invention is a facultative anaerobe, and is more suitable for industrial production.

Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. Both organisms produce near theoretical ethanol yields without expressing pyruvate decarboxylase

The invention provides a method for identifying mycobacterium tuberculosis. According to the invention, mycobacterium tuberculosis coding gene Rv1508c sequence is adopted as a gene sequence to be detected; and detection is carried out with primers (and probes) designed with the sequence as a detection target. The method provided by the invention has the advantages of good accuracy and high sensitivity. With the method, a novel scheme is provided for the identification of mycobacterium tuberculosis. The method can be used in biological strain identification, tuberculosis infection identification, and researches in respects such as efficacy and mycobacterium tuberculosis drug resistance in drug development processes.

The invention relates to a method for anaerobically degrading feather keratin with a microbial strain 18D-TA, belonging to the technical field of microorganisms. The method comprises the following specific steps of: A, treating feathers: cleaning feathers with water, putting into a drying oven of which the temperature is 45-55 DEG C, and drying for 2-3 days for later use; and B, inoculating the strain: performing high-temperature moist-heat sterilization on a culture medium and the feathers treated in the step A, inoculating the strain 18D-TA in the amount of 2-10 percent, and anaerobically standing and culturing at the temperature of 45-60 DEG C for 8-10 hours till feather down falls off, wherein the feathers are completely separated from feather stems 18-24 hours later, and the feather stems are degraded in different degrees. Compared with other aerobic bacteria, the strain used in the method has the advantages: more products can be accumulated, so that ventilation equipment is not required to be provided, and the energy consumption is lowered greatly; and meanwhile, the efficiency is high, the fermenting period is short, racemization of most amino acids is avoided, and the absorption applicability of a feather hydrolysis product is enhanced.

The present invention discloses a special fermented feed for laying hens capable of improving quality of eggshells and a preparation method thereof. The special fermented feed for the laying hens is prepared by uniformly mixing fermentation base materials and fermentation micro-organism liquid; the mixture is alternately subjected to aerobic fermentation and anaerobic fermentation to a pH value of4.2-4.7; the fermentation base materials comprise corns, wheat bran, soybean meal, stone powder, calcium dihydrogen phosphate and amylase; and the fermentation micro-organism liquid is prepared by expanding propagation of compound fermentation micro-organism strains of bacillus subtilis, lactobacillus acidophilus, pediococcus pentosaceus and saccharomyces cerevisiae. A nutrient-optimized microbial fermentation engineering technology is used to enable calcium and phosphorus required by the laying hens to participate in the fermentation, improves structural properties and material properties ofthe eggshells of the commercial laying hens, thus improves the eggshell quality and reduces an egg breaking rate. The bio-fermented feed ensures an egg production rate, prolongs an egg laying period,and has relatively high practical value for breeding enterprises.

The present invention provides a traditional Chinese medicinal fermented feed additive for promoting postpartum inflammatory rehabilitation and lactation of sows, a preparation method and an application thereof. The additive is prepared from the following components in parts by weight: 6-12 parts of anti-inflammatory and lactation-promoting traditional Chinese medicinal components, 10-15 parts ofsoybean meal, 30-50 parts of corn, 5-10 parts of finely selected dietary fibers, 5-10 parts of molasses, 0.5-2 parts of methionine, 0.1-2 parts of arginine, 0.5-1 part of tryptophan, 20-30 parts of purified water, and 1-3 parts of compound fermentation micro-organism strains. The traditional Chinese medicinal fermented feed additive can promote postpartum inflammatory rehabilitation, regulate theintestinal tract, increase feed intake and milk production, can provide rapid rehabilitation of the lactating sows, reduce labor stress, provide sufficient nutrients for sow lactation, reduce physicalloss, provide sufficient milk for growth of suckling pigs, increase the nutrient content in the milk and can effectively increase economic benefits of sow breeding.

The invention relates to a microbial strain and a fungicide and belongs to the technical field of microorganisms.The invention provides a photosynthetic bacterial strain HL which is identified as red pseudomonas (Rhodopseudomonas sp.) CGMCC No.11874.The strain is quick to grow and propagate, high in bacterial concentration and good in sundry bacterium contamination resistance capability and can degrade and remove various pollutants under the lighting or dark condition.The invention further provides a microbial fungicide containing the strain and a compound fungicide papered by the strain and another photosynthetic bacterial strain.The microorganisms can well grow in treated wastewater adopting the fungicide, occupy beneficial ecological niches and inhibit the growth and propagation of pathogenic microorganisms.

The invention discloses a method for reducing the cadmium content of rice grains in a cadmium-polluted rice field by using cadmium-resisting microorganisms, belonging to the technical field of biological remediation of heavy metal polluted soil. The method comprises the following steps: (1) screening and purifying the cadmium-resisting microorganisms to obtain two microorganism strains capable of resisting 300mg / L of cadmium; (2) identifying the cadmium-resisting microorganisms, wherein the cadmium-resisting microorganisms are identified to be aspergillus aculeatus and bacillus cereus; (3) preparing a cadmium-resisting microorganism culture substrate; and (4) applying the cadmium-resisting microorganism culture substrate in the rice field. According to the method disclosed by the invention, the screened cadmium-resisting microorganism strains come from rhizosphere soil of dominant plants of a cadmium polluted mine area, and have very good environment adaptability; the effect of the mixed strains of the aspergillus aculeatus and the bacillus cereus is remarkably better than the single application of the aspergillus aculeatus or the bacillus cereus, so that the repairing efficiency is improved; and the cost is low, the operation is simple and convenient and the effect is remarkable. The method is applicable to the biological remediation of the rice field oil with relatively light cadmium pollution.

The invention relates to the technical field of biological strains, particularly a Paecilomyces variotii strain and application thereof. The Paecilomyces variotii strain MM2 is collected to the China General Microbiological Culture Collection Center on July 8th, 2013, and the collection number is CGMCC No.7903. The application method comprises the following steps: adding the strain into any of straw-bran liquid culture medium, filter paper-inorganic salt liquid culture medium or PDA (potato dextrose agar) liquid culture medium, and culturing at 28-30 DEG C for 2-9 days to obtain the single-strain solid microbial inoculant. The Paecilomyces variotii strain can efficiently degrade and utilize cellulose, can be used as an external strain source for recycling lignocellulose solid waste, and can accelerate the degradation of the cellulose; the Paecilomyces variotii strain is separated from wine sediment compost and applied to wine sediment compost, thereby avoiding the problem of difficulty in assessment; and after being added, the microbial inoculant containing the strain effectively promotes the rotting of the wine sediment compost and accelerates the processing of the organic fertilizer.

The invention relates to a tobacco-derived cellulase-producing microbe strain and application thereof in tobacco. The invention is characterized in that the microbe strain is a Trichoderma harzianum TC10-13 strain which is collected by China General Microbiological Culture Collection Center on December 17th, 2013, and the collection number is CGMCC No.8587. The ITS sequence of the strain is the main characteristic reference for identifying the strain. The application method comprises the following steps: by using wheat straw powder or cabo powder as a carbon source and a yeast extract as a nitrogen source, carrying out liquid fermentation to obtain a crude enzyme solution, and proportionally spraying the fermentation crude enzyme solution onto the tobacco shred, sheet tobacco and cabo to obviously lower the cellulose content in tobacco. The cellulose degradation strain is convenient to apply and can enhance the inherent quality of the tobacco.

The invention discloses a method for degrading and removing microbial metabolism inhibitors existing in a hemicellulose diluted acid hydrolysate by using a detoxification active bacterial strain. The detoxification active bacterial strain comprises issatchenkia orientalis CCTCC NO:M 206098 and issatchenkia occidentalis CCTCC NO:M 203097, and both the ssatchenkia orientalis and the issatchenkia occidentalis can degrade the microbial metabolism inhibitors which comprise acetic acid, furfural and phenols substances and exist in the hemicellulose hydrolysate and have the characteristics of low pH value resistance and no utilization of pentose or sugar alcohol thereof. The detoxification active bacterial strain can be used for carrying out single bacterial detoxification treatment on the hemicellulose hydrolysate and can also generate xylitol or alcohol with fermented xylose for bacterial strain mixing and inoculating to the hemicellulose hydrolysate to carry out synchronous detoxification and fermentation. The biological detoxification method can effectively improve the fermenting property of the hemicellulose hydrolysate and improve the yield of fermentation products of the alcohol and the xylitol and has the advantages of environmental protection and energy saving.

Recombinant microbial strains are provided that express nitrilase enzyme and are useful as biocatalysts for the hydrolysis of nitrile-containing substrates. The recombinant cells are transformed with a foreign gene isolated from Acidovorax facilis 72W encoding a thermostable nitrilase enzyme that catalyzes the hydrolysis of nitrile-containing substrates to carboxylic acids under mild reaction conditions. The nucleotide sequence of the nitrilase gene and the deduced amino acid sequence encoded by the nitrilase gene are provided.

The present invention discloses a multi-micro-organism-strain compound micro-organism feed additive. The multi-micro-organism-strain compound micro-organism feed additive comprises probiotics, prebiotics and a carrier. The mass ratios of the probiotics, the prebiotics and the carrier are (2-6.5):(0.5-2):(2-6.5). The present invention also discloses an application of the multi-micro-organism-straincompound micro-organism feed additive in feeds. The addition amount of the multi-micro-organism-strain compound micro-organism feed additive is 0.1%-0.4%. The probiotics, prebiotics and carrier in the feed additive are scientific and reasonable in matching. After using the feed additive, the feed additive can maintain intestinal micro-ecological balance of poultry and livestock, improves the immunity and growth performance of the livestock and poultry bodies, improves the product quality, improves the feed utilization rate, improves product quality, reduces content of nitrogenous substances in livestock and poultry excrement and lowers concentration of ammonia and hydrogen sulfide in breeding places. At the same time, metabolites of the beneficial microorganisms have relatively strong inhibitory effects on growth and reproduction of pests of mosquitoes, flies, etc. and effectively improve the health environment of the breeding places.

The present invention discloses a method for promoting microbial cells to transport glucose, xylose and arabinose and application thereof in fermentation of biobased products. Five transport proteins provided by the present invention have capabilities of transporting glucose, xylose or arabinose. The method for promoting microbial cells to transport glucose, xylose and arabinose is characterized in that the transportproteins are guided into microbiological strains, the obtained reconstructed microbiological strains obtain or improve the capabilities in transporting glucose, xylose or arabinose, and further take use of glucose, xylose and arabinose to produce fuel ethanol and other biobased fermentation products.

The invention relates to a biological base coating material for increasing rainfall infiltration and reducing surface evaporation of shoal soil and a preparation method thereof and belongs to the technical field of soil organism improvement. According to the invention, the microorganism bacterial strain mucor mucedo from Jiangsu shoal saline alkali soil is separated and a biomass material is cured by the hyphae formed in the growth process thereof so as to form a felt coverage with an excellent airtight property, so that the surface moisture conduction is enhanced, the increasing of rainfall infiltration and the reducing of surface evaporation of the shoal saline-alkali soil are effectively promoted and the soil salt return speed is reduced. The rainfall infiltration volume is increased by two times, the soil water evaporation volume is reduced by 63%, the soil organic matter is increased by 5 times and the survival rate of the planted salt-tolerant plant sesbania is increased by 12 times.

The invention relates to a marine microorganism strain Y112 for producing alpha-cyclodextrin glycosyltransferase and enzyme produced by the strain. Upon 16SrDNA sequence analysis and in coordination with physiological and biochemical characteristics, the strain belongs to bacillus; and through sequencing, 16SrDNA sequence is about 1420bp long, and base composition is as shown in Figure 1. The marine microorganism strain is extensively applicable to medicine, food, chemical industry, cosmetics, industry, agriculture, analytical chemistry and like aspects.

The present invention discloses a preparation method of lactic acid bacterium fermented sour soybean milk and the lactic acid bacterium fermented sour soybean milk. The method comprises the followingsteps: deodorized soybean milk powder and sucrose mixing with water, stabilizer adding, homogenizing, pasteurizing, cooling, fermenting agent adding, stirring, fermenting and lactic acid bacterium fermented sour soybean milk obtaining. Streptococcus thermophilus, lactobacillus bulgaricus, lactobacillus acidophilus and bifidobacterium lactis at different ratios are mixed to ferment the sour soybeanmilk. The micro-organism strains are good in acid producing and viscosity generating, and the fermented sour soybean milk is thick, presents a wire drawing shape, and is smooth and lubricate in mouthfeel. Proteins in the sour soybean milk are pure plant proteins. The sour soybean milk is free of additions of milk powder and any animal protein products, and only soybean milk is needed to be fermented to produce the high-quality sour soybean milk.

The invention discloses a fermented product of a fungi strain for treating dementia. The fungi strain is separated from endophytic fungi of Huperzia serrata and belongs to fusarium oxysporum NSG-1 strain. After the passage of 20 generations in laboratory, the fungi strain has genetic stability. The amino sequence of the fungi strain is as shown in SEQ ID NO:1. The fungi strain provided by the invention is used as a biologic strain for treating vascular dementia and senile dementia; the excellent high-efficiency expression gene for generating huperzine a is transferred into the strains in other modes; more excellent characteristics can be acquired through gene modification; besides, further optimization for the bacterial strain can be achieved through mutation breeding; the research on the fungi strain has huge significance in biosynthesis industrial production of huperzine a, plant resource protection, settlement for the clinic first-line pharmacy requirement and reduction of medical expense for treating vascular dementia and senile dementia patients.

The invention relates to a natural trema levigata hand-mazz micro-organism soil-fixation carpet which comprises a substrate layer, a net layer and a paper mat layer; a plurality of pinholes are arranged on the substrate layer; the paper mat layer is arranged on the upside of the substrate layer; the net layer is embedded in surface of the paper mat layer; the net layer is constructed by the interleaving and threading of vertical net lines and transverse net lines; the vertical lines and the transverse net lines are natural fibrilia materials; the making method includes that the substrate layer is immersed in water; the knitted net layer is arranged horizontally; the micro-organism strain is sprayed on the surface of the paper mat layer; the paper mat layer is dried; the dried paper mat layer is arranged on the upside of the net layer horizontally; the loosened substrate layer after the immersion is evenly stacked on the net layer; the substrate layer is heated, compressed, perforated and cooled. The natural trema levigata hand-mazz micro-organism soil-fixation carpet has the advantages that the natural fiber material is adopted so that the ecological and environmental protection requirement is satisfied and the soil is improved; the soil-fixation carpet has good water saving property and facilitates the soil conservation; the making of the carpet is simple; the cost is low and the carpet is good for the popularization of the protection technology.