21 results about "Organism Strain" patented technology

Platforms, systems, and methods for genetic generalization in synthetic biology development. According to one aspect, there is provided a method for predicting performance associated with genetic edits, the method comprising: receiving, by a platform, information about a strain of a microorganism, wherein the information about the strain comprises information describing a plurality of genetic edits to a base strain of the microorganism; generating, by the platform, a set of genetic embeddings based on the information about the strain, wherein the generating comprises processing the information about the strain using one or more embedding models, wherein each of the one or more embedding models: receives the information about the strain of the microorganism as input; and applies computational transformations to the input using a corresponding embedding model to generate a multi-dimensional vector representation for each of the plurality of genetic edits.

The invention belongs to the technical field of microbial genetic engineering, and particularly relates to an alpha-2, 3-sialyltransferase mutant and application thereof. The a-2, 3-sialyltransferase mutants V175N, P198A, G173D, D174Q, F196K and A315H are obtained by performing site-specific mutagenesis on six amino acids in an a-2, 3-sialyltransferase amino acid sequence coded by a gene derived from Campylobacter jejuni (OH4384), the yield of 3 '-sialyllactose is remarkably increased by single-point mutagenesis of the V175N, the yield of 3'-sialyllactose is remarkably increased by single-point mutagenesis of the V175N, the yield of 3 '-sialyllactose is remarkably increased by single-point mutagenesis of the P198A, the G173D, the D174Q, the F196K and the A315H, the optimal recombinant microbial strain 3 '-SL-09 (V175N / P198A / D174Q) is obtained through combination of multiple mutation sites, the yield can reach 5.91 g / L, the bottleneck problem of low catalytic efficiency of sialyltransferase is effectively solved, and an enzyme element with excellent performance is provided for high-efficiency and low-cost biological manufacturing of 3'-SL.

The invention belongs to the technical field of microbial genetic engineering, and particularly relates to an alpha-2, 6-sialyltransferase mutant and application thereof. The a-2, 6-sialyltransferase mutants M133L, E140D, D228H, N289D and G322Q are obtained by carrying out site-specific mutagenesis on five amino acids in an a-2, 6-sialyltransferase amino acid sequence coded by a gene derived from Photobacterium sp.JT-ISH-224, and compared with wild type alpha-2, 6-sialyltransferase, the yield of the single-point mutant 6 '-SL of the E140D and the yield of the single-point mutant 6'-SL of the G322Q are increased by 87.80% and 78.01% respectively. The yield of the optimal recombinant microbial strain 6 '-SL-7 (E140D / G322Q) obtained by mutation site combination can reach 1.471 g / L, the bottleneck problem of low catalytic efficiency of sialyltransferase is effectively solved, and an enzyme element with excellent performance is provided for high-efficiency and low-cost biological manufacturing of 6'-SL.

The invention discloses a strain of Bacteroides ovatus L2 and application of the strain of Bacteroides ovatus L2 in a product for preventing and treating vomitoxin toxicity. The strain is preserved in Guangdong Microbiological Culture Collection Center, the preservation number is GDMCC.No: 67205, and the preservation date is November 4, 2025. The strain is separated and purified from a healthy adult excrement sample, has the characteristics of good acid resistance, cholate resistance, gastrointestinal fluid resistance and the like, and is free of hemolysis and good in safety. The strain can effectively relieve the toxic effect induced by vomitoxin, mainly inhibits inflammatory factors and promotes expression of intestinal barrier functional proteins, can be applied to products of microecologics for preventing and treating DON toxicity, and has a good application prospect.

This invention relates to a method for efficiently spraying soil conditioner to treat saline-alkali land using composite microorganisms, belonging to the field of soil improvement technology. The method involves screening salt-tolerant functional microbial strains to prepare a composite microbial agent, which is then mixed with carrier materials such as diatomaceous earth, soybean meal, yeast metabolites, and well-rotted farmyard manure in a specific ratio to form a conditioner for application. The conditioner is applied using a two-stage spraying method, combined with deep tillage pretreatment and controlled temperature and humidity conditions, significantly improving microbial activity and soil condition uniformity. Experimental data show that after applying this method, the soil pH value decreased to 7.3-7.5, the salt content decreased to 0.1%-0.2%, and the organic matter content increased to 18-22 g / kg, achieving efficient ecological restoration and sustainable utilization of saline-alkali land.

This application relates to the field of microbial strain technology, specifically disclosing a Bacillus thuringiensis HP-SYJ2 strain and its applications. The Bacillus thuringiensis HP-SYJ2 strain is deposited at the China Center for Type Culture Collection (CCTCC), accession number: CCTCC NO: M2025343, deposit date: March 3, 2025, address: Wuhan University, Wuhan, China. The strain of this application can be used to prepare insecticides for controlling underground pests, which is of great significance for the biological control of plant diseases and pests.

The application relates to the technical field of microbial strain preservation, in particular to a microbial strain preservation device which comprises a freezer, a moving assembly and a rotating assembly, the freezer is provided with a box door, a fixing seat is arranged in the freezer, the end of the fixing seat is provided with a supporting disc, a plurality of groups of test tube racks are fixedly connected to the supporting disc, and the bottom end of the supporting plate is fixedly connected with a connecting shaft; the moving assembly is arranged in the freezer and is used for driving the supporting disc to displace; and the rotating assembly is used for driving the connecting shaft to rotate, so that the supporting disc rotates. The moving assembly and the rotating assembly are arranged, the connecting shaft drives the supporting plate to move a small distance through the action of the moving assembly, part of the position of the supporting disc is moved to the outside of the freezer, then the connecting shaft drives the supporting disc to rotate through the cooperation of the rotating assembly, and then the strain at any position on the supporting plate can be taken and placed, so that the operation is more convenient.

The invention relates to a recombinant microbial strain for de novo synthesis of gardenin B and application of the recombinant microbial strain in production of polymethoxylated flavone compounds, and belongs to a cross technology of synthetic biology and organic synthetic chemistry. The invention designs and constructs a heterozygous gardenin B biosynthesis pathway, and obtains a microbial strain for synthesizing gardenin B from the beginning. By screening F8H and redox partner types and regulating and controlling subcellular localization, an F8H-redox partner combination with optimal space and type is determined, and an engineering strain of which the gardenin B yield is greatly improved is obtained. The efficient synthesis method of the polymethoxylated flavonoids is established by selecting recombinant microorganisms for synthesizing gardenin B from de novo to perform co-culture fermentation or chemical conversion of fermentation products.

The application discloses a coal gangue microbial active substrate for planting and a preparation method thereof. The application can dissociate effective mineral elements in coal gangue through the decomposition and activation of microorganisms, and can form a microbial substrate for planting by cooperating with added biogenic solid waste such as biogas residue, coconut husk, poultry manure and vinegar residue. The prepared microbial substrate contains active microbial strains which can continuously decompose and activate coal gangue, and can continuously release mineral elements necessary for plant growth, and can decompose organic matters in biogenic solid waste. Through the added biogenic solid waste, a certain particle size, loose and breathable physical structure and stable physicochemical properties are formed, and the physical structure can stably provide nutrient elements and organic matters for plants in the plant growth period. The microbial active substrate prepared by using the application can slowly provide necessary nutrient substances for plants in the cultivation period, and can better promote plant growth.

The application discloses a kind of fluorine-resistant trichoderma asperellum and the fungicide prepared by it, belong to microbial strain and application technical field.The strain is named Trichoderma asperellum M700, has been preserved in China typical culture preservation center (CCTC), and its preservation number is CCTCC NO: M 2024579, and the preservation date is March 27, 2024.The beneficial effect: the trichoderma asperellum strain of the application Trichoderma asperellum. Colony growth early stage presents white mycelium, later generation green spore, causes the body to present yellow green, green, aerobic, can survive at ph 3.0-10.0, temperature is 25-28 DEG C.The strain has very good resistance, and can grow when the fluorine ion concentration is below 800 mg / L.Can very good dispel fluorine element in water body.

Microbial starter cultures. More specifically, a method for preparing a microbial culture such as a lactic acid bacteria (LAB) starter culture wherein at least one microbial strain such as a lactic acid bacteria and at least one inactivated yeast strain is inoculated in a culture medium.

This invention belongs to the field of microbial fermentation technology, specifically relating to a new strain of Pichia pastoris (Kudelazwiel). Pichia Kudryavzevii ) and its applications. To stabilize and target the production of ethyl acetate in baijiu (Chinese liquor), this invention screens, isolates, and identifies a strain of *Pichia pastoris* capable of high ethyl acetate production from soy sauce-flavored baijiu mash. Pichia kudriavzevii LJ-5, with accession number CCTCC NO:M20252599, is a strain that enhances the fermentation of lees. In baijiu brewing, it can be used to produce ethyl acetate, ethyl lactate, and other flavor compounds, especially significantly increasing the content of ethyl acetate produced during fermentation. Therefore, this strain can be applied to baijiu production as a functional microbial strain to improve baijiu quality.

The application discloses a Sphingobium yanoikuyae and application thereof in decomposing ascr#18. The Sphingobium yanoikuyae 25D is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on May 10, 2023, and the address of the GDMCC is the 5th floor of the building 59, the courtyard 100, the Middle of the Martyrs, Guangzhou, the postal code of the Guangdong Academy of Microbiology is 510070, and the preservation number is GDMCC No: 63454. It is found for the first time that the Sphingobium yanoikuyae 25D is a southern root-knot nematode accompanying bacterium and has the function of decomposing ascr#18.

The present disclosure provides a method for designing microbial consortia for fermentation to approximate a targeted organoleptic profile of a fermented outcome by training and applying a machine learning model using microbial-substrate interaction data under various fermentation parameters. The model, by taking fermentation parameters predicts metabolites produced by combinations of the candidate microbial strains with candidate substrates under various fermentation parameters and selects at least one microbial strain, substrate, and fermentation parameter for the microbial consortia. The present disclosure also provides a method for predicting an organoleptic profile of a fermented outcome based on the inputs of fermentation parameters, microbial strains, and substrates.

A method for treating a microbial infection in a subject is provided that includes administering to the subject a composition including a therapeutically effective amount of at least one microbial strain adapted to perform a competitive Stickland fermentation or provide a pathway or mechanism of proline utilization or depletion. Also provided is a composition for use in treating a microbial infection, a synthetic microbiota or synthetic microbiota transplant including such composition, the use of such composition in the manufacture of a medicament for treating a microbial infection, and a medical kit for treating a microbial infection including the synthetic microbiota transplant and an instruction for use thereof.

The present disclosure discloses a method for removing TBBPA in water, a microbial strain and a microbial agent, wherein the microbial strain is a domesticated Burkholderia cepacia strain, which is named Y17 with a conservation number GDMCC No. 62153. The microbial agent and the method for removing TBBPA in water with the microbial agent are that Y17 strains are colonized on the surface and pore channels of biochar, TBBPA in water is used as a carbon source, air and dissolved oxygen are used as oxygen sources, biochar provides the strains a growth microenvironment for degrading TBBPA in water, the strains are subjected to aerobic growth in water, and bio-enhanced degradation of TBBPA in water is performed by continuously degradation reaction. The removal method and the microbial strain as well as the microbial agent are high in degradation efficiency, environmental-friendly and low in cost, and can meet requirements on large-range promotion and application.

The invention discloses a microbial marker for predicting the occurrence risk of endometrial cancer and application of the microbial marker, an endometrial microbiome is evaluated to determine that differential microbial genus is lactobacillus, an endometrial cancer microbial strain library is constructed, it can be known from the strain library that a large number of bacteria are inert lactobacillus, and the bacterial strain library can be used for predicting the occurrence risk of the endometrial cancer, so that the occurrence risk of the endometrial cancer can be predicted, and the occurrence risk of the endometrial cancer can be predicted. Therefore, the endometrial cancer differential microbial flora is determined to be lactobacillus inertus, and the lactobacillus inertus is cultured to obtain the microbial marker for detecting the occurrence risk of the endometrial cancer. The microbial marker for predicting the occurrence risk of the endometrial cancer, provided by the invention, is lactobacillus inertus, and can be used for development and application of clinical detection kits for the endometrial cancer in the future by designing specific strain primers; the endometrial cancer microbial strain library provided by the invention can be used for designing potential microbial marker strains of endometrial cancer occurrence risks, and a method for co-culturing the microbial strains and tumor cells can also be used for verifying the influence of the microbial strains on different tumor cells.

This invention belongs to the field of crop straw treatment technology, specifically disclosing a low-carbon, multifunctional bio-compound fertilizer made from straw, its preparation method, and its application. The bio-compound fertilizer provided by this invention, through the scientific formulation of functional microbial strains and the use of microbial immobilization technology to load the fertilizer, combines highly efficient strains with EM bacteria and nitrification inhibitors, thereby improving the straw return-to-field effect, sequestering carbon and reducing emissions, and promoting sustainable agricultural development.

The application discloses a recombinant microbial strain with high emodin-8-methyl ether production and a construction method and application thereof. The recombinant microbial strain is a yeast strain which produces emodin-8-methyl ether after being cultured in a yeast extract powder peptone dextrose medium through shake flask fermentation, with a yield of 409 mg / L, produces emodin-8-methyl ether after being cultured in a basic salt medium through shake flask fermentation, with a yield of 383 mg / L, and produces emodin-8-methyl ether after being cultured in a 5L bioreactor containing a basic salt medium, with a yield of 11.35 g / L. The application discloses a biosynthesis method for high emodin-8-methyl ether production after optimization. The application lays a foundation for industrialized large-scale fermentation production of emodin-8-methyl ether, and has important economic value and social benefits.

The invention discloses an application of a product of Limosilbacillus reuteri LR1 (Limosilbacillus reuteri LR1) for producing gamma-aminobutyric acid (gamma-aminobutyric acid) from pigs in prevention and treatment of vomitoxin toxicity and a preparation method of the Limosilbacillus reuteri LR1. The strain is preserved in Guangdong Microbiological Culture Collection Center, the preservation number is GDMCC.No: 64522, and the preservation date is April 17, 2024. The strain is separated and purified from a pig cecum content sample, the performance of gamma-aminobutyric acid produced by fermentation of the strain is stable, and the yield of gamma-aminobutyric acid is 0.6 g / L. The strain has a good protection effect on prevention and treatment of vomitoxin toxicity, can be applied to preparation of products for prevention and treatment of animal vomitoxin toxicity and strains for fermentation production of GABA, can be applied to different fields of veterinary microecologics, feed production and the like, and has a wide application prospect.

Microbial starter cultures. More specifically, a method for preparing a microbial culture such as a lactic acid bacteria (LAB) starter culture wherein at least one microbial strain such as a lactic acid bacteria and at least one inactivated yeast strain is inoculated in a culture medium.