Method for producing DHA by Crypthecodinium cohnii industrial fermentation

A technology of docosahexaenoic acid and Cryptidium koii, applied in the field of mixed oils containing docosahexaenoic acid, can solve the problems of waste extraction of DHA, complicated production process, high cost, etc., and achieve lower consumption threshold , low cost, and the effect of reducing fermentation costs

Active Publication Date: 2009-09-23
湖北福星生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current marine fishery resources are nearly exhausted, and the extraction of DHA from fish oil causes great waste
The cultivation of micro-bath requires light and carbon dioxide, the production process is complicated and the cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1. Shake flask medium screening

[0050] Formula a enhanced slow-release nitrogen source-soybean meal powder hydrolyzed juice (g / 100ml culture medium)

[0051] project

[0052] Recipe b fortified growth factor-bean sprouts juice (g / 100ml culture medium)

[0053] project

[0054] Recipe c fortified with hematitol and arginine (g / 100ml culture medium)

[0055] project

[0056] Formula d comprehensive experiment (g / 100ml culture medium)

[0057] project

[0058] Formula e blank control experiment (g / 100ml culture medium)

[0059] project

[0060] Formula f common fermentation medium formula (g / 100m1 medium)

[0061] project

[0062] The above hydrolyzed juice of soybean meal powder is prepared by the following method: dissolve soybean meal powder in water, adjust the pH to 9.0-10.0, and raise the temperature to 75°C, keep it warm for 9 hours, filter the clear liquid to obtain soybean meal powder h...

Embodiment 2

[0072] Example 2 50 liters tank fermentation culture Cryptidinium koii to produce DHA

[0073] Medium formula (g / 100ml medium)

[0074] project

content

project

content

Soybean Meal Powder Hydrolyzed Juice

6.0

sodium glutamate

1.0

Yeast extract

0.5

corn syrup

0.5

glucose

6.0

Magnesium Sulfate Heptahydrate

0.3

Potassium dihydrogen phosphate

0.4

sea ​​salt

1.0

[0075] The above hydrolyzed juice of soybean meal powder is obtained by the following method: dissolve the soybean meal powder in water, adjust the pH to 9.0, and raise the temperature to 80°C, keep it warm for 8 hours, filter the clear liquid to obtain the hydrolyzed juice of soybean meal powder; the weight ratio of soybean meal powder and water is It is 1:3. The preparation of bean sprouts juice is with embodiment 1.

[0076] Add water to the required volume, adjust the pH to natural, sterilize a...

Embodiment 3

[0092] Example 3 500 liters tank fermentation culture Cryptoria koii to produce DHA

[0093] Medium formula (g / 100ml medium)

[0094] project

content

project

content

Soybean Meal Powder Hydrolyzed Juice

8.0

bean sprouts juice

3.0

Yeast extract

0.5

sodium glutamate

1.0

glucose

6.0

corn syrup

0.5

Potassium dihydrogen phosphate

0.4

Magnesium Sulfate Heptahydrate

0.3

sea ​​salt

1.0

[0095] The above hydrolyzed juice of soybean meal powder is obtained by the following method: dissolve the soybean meal powder in water, adjust the pH to 10.0, and raise the temperature to 70°C, keep it warm for 10 hours, filter the clear liquid to obtain a slow-acting nitrogen source; wherein the weight ratio of soybean meal powder and water is It is 1:10. The preparation of bean sprouts juice is with embodiment 1.

[0096] Add water to the required volume, ad...

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PUM

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Abstract

The invention discloses a method for producing grease containing triglyceride type DHA by Crypthecodinium cohnii industrial fermentation. The method comprises the following steps: taking the Crypthecodinium cohnii as a strain, employing a culture medium comprising a carbon source, a nitrogen source and inorganic salts, and obtaining the DHA grease by fermentation; the nitrogen source contains hydrolytic liquor of slow-release bean pulp powder as nitrogen source. The grease produced by the fermentation contains more than 50% of DHA, among which EPA content is below 1%, and the DHA productivity reaches 4.6g/L per day. The DHA has high output, high purity, safe and reliable quality, low production cost, stable production, and little or no EPA; The method can produce environment-friendly and safe high DHA content triglyceride type DHA grease in quantity with low cost.

Description

technical field [0001] The invention relates to a microbial fermentation production method, in particular to the fermentation production of mixed oil containing docosahexaenoic acid by using Cryptidium koii strains. Background technique [0002] Docosahexaenoic acid (cis-4, 7, 10, 13, 16, 19-docosahexaenoic acid, referred to as DHA) is an omega 3 series of long-chain polyunsaturated fatty acids, and is an important structural substance of the brain and retina. It can regulate the function of the central nervous system, prevent and treat cardiovascular diseases, eliminate inflammation, and inhibit cancer. [0003] Commercial sources of DHA are currently mainly fish oils and microalgae. The output and DHA content of fish oil are unstable, and the extract is mixed with fishy smell, and the DHA-containing mixed oil extracted from fish oil also contains eicosapentaenoic acid (EPA). According to research, EPA has a positive effect on the development of infants and young children....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/89
Inventor 徐丛英夏德才宁超美林绍朗吴艳荣解秀娟
Owner 湖北福星生物科技有限公司
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