42 results about "Cibotium barometz" patented technology

The invention discloses a prothallus proliferation culture medium and a rapid propagation method for rare and endangered plant cibotium barometz. The prothallus proliferation culture medium of cibotium barometz is used for accelerating the proliferation of gametophytes, and the prothallus maturation time is shortened; meanwhile, the invention provides a rapid propagation method of cibotium barometz, which comprises the following steps: carrying out artificial sterile culture on cibotium barometz spores, rapidly germinating in a culture bottle to form prothalli, culturing on a prothalli proliferation culture medium, inducing and transforming gametophytes in a specific matrix outside the bottle to form sporophytes by virtue of an incomplete tissue culture method, and transplanting. According to the method, the formation of prothallium is accelerated, the sporophyte conversion rate is increased, the reproduction rate is increased while the domestication and reproduction of sporophyte seedlings are accelerated, a large number of cibotium barometz seedlings which grow healthily can be obtained, the survival rate reaches 90%, the spore breeding period is obviously shortened, the operation requirement is not strict, and the method is suitable for large-scale popularization and application. The technical problems that the seedlings in the tissue culture bottle are easy to pollute, fragile and low in seedling rate are solved.

The invention provides a method for breeding spores of cibotium barometz and rejuvenating young spores. The method for artificially cultivating the Cibotium barometz, provided by the invention, comprises the following steps: separately planting sporophyte seedlings of the Cibotium barometz to be rejuvenated into a matrix, and carrying out rejuvenation culture; wherein the substrate is a mixture formed by mixing leaf mold and peat soil according to the volume ratio of (1-1.5): 1 or 1: 1, or a mixture formed by mixing leaf mold, white vermiculite and garden soil according to the volume ratio of 4: 1: 3. The method is low in cost, simple in technology, high in yield, high in seedling growth speed, suitable for large-scale batch production, capable of effectively meeting the rapid breeding requirement of the golden dog and capable of being widely applied to the fields of landscaping, medicinal production, scientific research and the like.

The invention provides a soil sowing seedling raising method for cibotium barometz. The soil sowing seedling raising method of the cibotium barometz comprises the following steps: taking peat soil as a matrix, sowing spores of the cibotium barometz into the matrix, and culturing until sporophyte seedlings are obtained. According to the method, on the premise that the seedling quality is guaranteed, the seedling raising period is shortened, good growth conditions are provided for germination and development of the spores of the cibotium barometz, the seedling raising efficiency is improved, and the important practical value is achieved.

The invention provides a traditional Chinese medicine composition for treating gout and a preparation auxiliary device.The traditional Chinese medicine composition comprises liquorice, astragalus membranaceus, semen plantaginis, lysimachia christinae hance, cibotium barometz and oriental wormwood, and the medicine properties of the traditional Chinese medicine composition for treating the gout are slightly cold, bitter and pungent in taste and free of toxic and side effects. Besides being used for treating gout symptoms, the traditional Chinese medicine also has the effects of inducing diuresis, clearing heat and promoting diuresis, removing jaundice, clearing liver and improving vision, clearing lung and eliminating phlegm and the like. Besides, in the process that the extraction liquid and the solid residues are separated through rotation of the centrifugal cylinder in the auxiliary device, a balance weight plate and a pressing plate are thrown outwards under the action of centrifugal force at the same time till the fixed residues are pressed to the side wall of the classification barrel, extrusion on the solid residues is formed, the extraction liquid permeating into the solid residues is fully extruded out, and the solid residues are separated. The extraction amount of an extraction liquid formed by extracting the traditional Chinese medicine composition is increased, and waste is avoided.

The invention relates to a direct seeding and seedling raising method for cibotium barometz spores, and belongs to the technical field of plant planting. The invention provides a direct seeding seedling raising method for cibotium barometz spores. The method comprises the following steps: directly seeding the spores to obtain sporophyte bud seedlings; transplanting the sporophyte bud seedlings to obtain sporophyte seedlings; seedling hardening is carried out on the sporophyte seedlings; the sporophyte seedlings are subjected to seedling hardening when the height is 10-15 cm and the number of leaves is 3-4. By means of the direct seeding seedling raising method, the seedling yield is remarkably increased, the seedling yield of the obtained sporophyte seedlings is 85% or above, large-scale industrial popularization and planting can be conducted, and the market supply amount is guaranteed.

The invention belongs to the technical field of cibotium barometz breeding, and particularly relates to a method for artificially breeding cibotium barometz by using spores, which comprises the following components in parts by weight: 100 parts of field soil, 20-25 parts of sawdust, 10-15 parts of perlite, 10-12 parts of plant ash, 0.05-0.15 part of manganese sulfate and 0.3-0.5 part of sodium selenite; spores germinate, and the environment temperature is 24-26 DEG C; and transplanting the germinated seedlings into a greenhouse for cultivation. According to the method, high spore germination rate and seedling survival rate are ensured, and the transplanted seedlings can grow smoothly.

The invention discloses a cibotium barometz sporophyte transformation method and system based on multi-factor coupling regulation, and relates to the technical field of biology. The method comprises the following steps: synchronously acquiring environment data and sporophyte image data; preprocessing the environment data, performing feature extraction on the image data, and splicing the processed data into a comprehensive feature vector; quantifying the weight, correlation degree and interaction level among the environmental factors through an entropy method, grey correlation analysis and a coupling coordination degree model; constructing an improved CNN-LSTM neural network, extracting features through a double-branch structure, fusing a coupling analysis result to carry out cross-modal fusion, and training to obtain a prediction model; when the predicted conversion rate is lower than a threshold value, environment parameters are automatically adjusted until conversion is completed; according to the method, the problems of environmental factor data isolation and regulation lag in the prior art are solved, accurate prediction and intelligent regulation of the sporophyte transformation process are realized, and the transformation success rate and the production efficiency are remarkably improved.

The invention belongs to the technical field of traditional Chinese medicine compositions, and provides a powder decoction plaster for treatment and health care of rheumatism bone diseases and a preparation method thereof, the powder decoction plaster comprises the following raw materials by mass: 20-25 parts of ox horn slices, 10-25 parts of light wheat, 20-25 parts of yellow ginger, 20-25 parts of dried ginger, 2-10 parts of notopterygium root, 3-10 parts of sichuanese peppercorn, 0.1-0.5 part of safflower, 0.1-2 parts of cortex acanthopanacis, 0.1-0.5 part of licorice, 1-5 parts of rhizoma cibotii, and 1-5 parts of cortex dictamni. The traditional Chinese medicine disclosed by the invention is decocted into ointment to be applied to an affected part, has the effects of promoting rehabilitation and health, and is used for treatment and health care of lumbar disc herniation, cervical spondylosis, fracture, arthritis, synovitis, hyperostosis, scapulohumeral periarthritis, lumbar disc herniation, sprain and contusion and soft tissue contusion.

The invention belongs to the technical field of traditional Chinese medicine preparations, and provides an external tincture patch for treating lumbago in the traditional Chinese medicine internal medicine department and a preparation method, the tincture patch is composed of a solvent and effective components, and the effective components comprise 10-12 g of scalded rhizoma cibotii, 10-12 g of radix angelicae pubescentis, 10-12 g of salted eucommia ulmoides, 8-12 g of achyranthes bidentata, 6-10 g of safflower carthamus, 8-10 g of wine-processed ligusticum wallichii, 12-15 g of lycopodium clavatum, 12-15 g of impatiens balsamina, 6-9 g of frankincense, 6-9 g of myrrh and 3-5 g of natural borneol. The external tincture patch for the lumbago in the traditional Chinese medicine internal medicine department sequentially comprises a backing layer, a medical pressure-sensitive adhesive layer and an anti-seepage ring from bottom to top, an adsorption layer for soaking a tincture to a saturated state is arranged on the inner side of the anti-seepage ring, and the preparation method of the external tincture patch comprises the steps of traditional Chinese medicine processing, tincture extraction, patch forming and the like. The medicine is efficiently extracted through an ethanol solvent, transdermal absorption of the medicine is promoted, and the product is simple and convenient in preparation process, low in cost and convenient for a patient to use by himself or herself and carry daily.

The invention belongs to the technical field of traditional Chinese medicine preparations, and particularly relates to traditional Chinese medicine compound paste for treating spinal lesions and a preparation method thereof.The traditional Chinese medicine compound paste is prepared from rhizoma drynariae, rhizoma cibotii, herba taxilli, fructus amomi, cortex cinnamomi, tripterygium wilfordii, radix aconiti carmichaeli, garden balsam stem, poria cocos, rhizoma polygonati, propolis, turpentine and traditional Chinese medicine fermentation components, the black sesame, the cornu cervi pantotrichum slices, the earthworms and other traditional Chinese medicines after fermentation treatment are rich in organic selenium and mineral elements and are used for providing nutrition and promoting metabolism, and the traditional Chinese medicine compound cream prepared through cold soaking, ultrafiltration and oil-water two-phase extraction can effectively retain micromolecule polypeptide and other active substances and achieve a multi-target and high-penetration long-acting transdermal treatment effect.

The application provides a Cibotium barometz root endophytic fungus Pseudosperma yunnanense JMG086 and a separation method and application thereof belong to the technical field of bacterial disease control. The Cibotium barometz root endophytic fungus Pseudosperma yunnanense JMG086 is named as Pseudosperma yunnanense , and the preservation number is CCTCC NO: M 20251771. It is found through experiments that the Cibotium barometz root endophytic fungus Pseudosperma yunnanense JMG086 liquid fermentation broth extract has a strong inhibitory effect on the growth of Agrobacterium tumefaciens, Pectobacterium carotovorum and Ralstonia solanacearum, and can effectively prevent and control plant diseases.

The invention relates to a method for evaluating the effect of formula fertilization of cibotium barometz and a formula fertilization method. The method comprises the following steps: S1, preparing fertilizer solutions with different contents of nitrogen, phosphorus and potassium; s2, the test group adopts the fertilizer solution to respectively apply fertilizer to the same specification of cibotium barometz; s3, picking leaves of the cibotium barometz, and measuring the content of nitrogen, phosphorus and potassium and the content of chlorophyll in the leaves; s4, selecting the fertilization formula adopted by the test group with the highest chlorophyll content in the leaves as a predetermined target, verifying the leaf nitrogen, phosphorus and potassium content of the test group corresponding to the predetermined target, if the leaf nitrogen, phosphorus and potassium content of the test group corresponding to the predetermined target is higher than that of the control group, determining that the verification is passed, and if the leaf nitrogen, phosphorus and potassium content of the test group corresponding to the predetermined target is not higher than that of the control group, determining that the verification is passed. Taking the predetermined target as a screening result of formula fertilization; otherwise, returning to the step S1 for re-screening. According to the method, a fertilization formula capable of remarkably promoting growth of the cibotium barometz can be obtained through screening, the planting difficulty of the cibotium barometz is reduced, and large-area planting of the cibotium barometz is facilitated.

This invention belongs to the field of drug detection, specifically relating to a method for constructing a fingerprint spectrum of Cibotium barometz and a method for determining the content of its active ingredients. The construction method includes the preparation of a test solution and detection using high-performance liquid chromatography (HPLC) to obtain the fingerprint spectrum of Cibotium barometz. This invention establishes a comprehensive and rapid method for detecting Cibotium barometz drug preparations, which is of great significance for its comprehensive quality detection and overall quality control. The characteristic spectrum obtained by this construction method has at least 12 common characteristic peaks, a stable baseline, and good peak resolution. Furthermore, the relative retention times of each characteristic peak are calculated based on protocatechuic acid, which is beneficial for the comprehensive quality detection and overall quality control of Cibotium barometz preparations, and helps improve the safety and stability of drug use.

This invention relates to a method for the germination of Cibotium barometz spores and a method for the propagation of Cibotium barometz, belonging to the field of plant culture technology. The propagation method of this invention includes the following steps: disinfection: disinfecting Cibotium barometz spores with a 10% sodium hypochlorite solution for 5-10 minutes; spore germination: the germination medium used includes 1 / 4 MS medium, 4.5-7 g·L⁻¹. ‑1 Agar and 0-30 g·L ‑1 Sucrose; Sporophyte differentiation: The sporophyte differentiation media used included 1 / 2 MS medium and 0-1 mg / L... ‑1 6-BA, 0.5-1 mg·L ‑1 2,4-D, 4.5-7 g·L ‑1 Agar and 25-30 g·L ‑1 Sucrose; Sporophyte strengthening: The sporophyte strengthening medium used included 1 / 4 MS medium, 4.5–7 g·L⁻¹. ‑1 Agar, 30 g·L ‑1 Sucrose and 1-2 g·L ‑1 Activated carbon; hardening and transplanting: After the tissue culture seedlings are sealed, the seal is gradually lifted, and they are then transferred to a specific mixed substrate for cultivation to obtain commercial seedlings. This method can improve the spore germination rate by using a specific sterilization procedure, and achieves the effect of producing more and better seedlings by using specific culture media and hardening preparations at each stage.

The invention provides a spore propagation method of cibotium barometz, and relates to the technical field of plant propagation. The method provided by the invention comprises the following steps: sowing Cibotium barometz spores in a composite culture medium, culturing under the conditions of natural illumination and normal temperature, promoting gametophyte transformation by adopting atomized water spraying instead of traditional oscillation insemination, and culturing seedlings in combination with a non-woven fabric seedling culture container. By means of environmental conditions such as natural light and the like, the bionic technology is adopted for breeding, daily management and protection are simple and economical, low-cost, rough-management and efficient batch seedling raising are achieved, the rate of converting gametophytes into sporophytes is greatly increased, the period from spores to transplantable seedlings of cibotium barometz is shortened to 70-80 days, the transplanting survival rate can be increased to 100%, and the survival rate of cibotium barometz is increased to 50%. And a seedling raising guarantee is provided for batch planting of cibotium barometz.

The invention belongs to the technical field of traditional Chinese medicines and relates to a traditional Chinese medicine composition for treating chronic muscle and bone diseases as well as a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by mass: 4 to 50 parts of rhizoma drynariae, 3 to 30 parts of rhizoma cibotii, 1 to 20 parts of fructus ligustri lucidi, 1 to 20 parts of caulis spatholobi, 1 to 20 parts of herba pyrolae and 1 to 20 parts of radix cyathulae. The rhizoma drynariae, the rhizoma cibotii, the glossy privet fruit, the caulis spatholobi, the herba pyrolae and the radix cyathulae are compatible and have a synergistic effect of monarch, minister, assistant and guide, so that the traditional Chinese medicine composition has multiple effects of tonifying kidney and strengthening bone, nourishing and promoting blood circulation and activating meridians to stop pain, and can be used for improving bone density, regulating bone metabolism, increasing muscle force, relieving bone joint pain and improving spinal joint functions; and chronic muscle and bone diseases such as osteoporosis and degenerative osteoarthritis can be effectively treated.

The invention discloses a formula for treating gout and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. The formula consists of caulis spatholobi, ligusticum wallichii, salvia miltiorrhiza, achyranthes bidentata, vinegar rhizoma corydalis, hot rhizoma cibotii, desert cistanche, vinegar turtle shell, liquidambar formosana hance, astragalus membranaceus, angelica sinensis, fructus lycii, centipede, scorpion, hippocampus and processed semen strychni. The preparation method comprises two dosage forms of honeyed boluses and medicinal liquor, wherein the honeyed boluses are prepared by crushing and sieving medicinal materials, and mixing the sieved medicinal materials with tender honey refined at 105-115 DEG C according to a ratio of 1: 1-1: 1.5; the medicinal liquor is prepared by soaking the medicinal materials in white spirit of 50-60 degrees for 30-60 days. Through synergism of multiple effects of promoting blood circulation to remove blood stasis, tonifying liver and kidney, dredging collaterals and removing stasis and the like, the gout symptom can be effectively relieved, the two dosage forms meet the requirements of different patients, and clinical verification shows that the traditional Chinese medicine composition is safe and effective.

The invention discloses an application of a rhizoma cibotii extract and a medicament for inhibiting prostatic hyperplasia. According to the present invention, the prostatic hypertrophy inhibition effect is provided, and the new practical value of the cibotium barometz is increased.

The present invention relates to a traditional Chinese medicine composition for treating pain and discomfort caused by cervical and lumbar intervertebral disc herniation. A traditional Chinese medicine composition for treating cervical and lumbar diseases, comprising the following raw materials in parts by weight: rhizoma cimicifugae, bupleurum chinense, millettia reticulata benth, lonicera japonica thunb, atractylodes macrocephala, white peony root, oyster, endothelium corneum gigeriae galli, achyranthes bidentata, eucommia ulmoides, cibotium barometz, and prepared rehmannia root. The traditional Chinese medicine composition for treating pain and discomfort caused by cervical and lumbar intervertebral disc herniation according to the present invention has good application effects in the conservative treatment of pain and discomfort caused by cervical and lumbar intervertebral disc herniation, and can effectively improve the pain and discomfort symptoms of patients with cervical and lumbar diseases.

The field cibotium barometz native plant mature spore collector comprises a conical seat, the conical seat is connected with a lifting structure, the top of the lifting structure is connected with a bottom plate, side plates are fixed to the two sides of the bottom plate, supporting strips are fixed to the tops of the side plates, the inner sides of the tops of the supporting strips are concave downwards to form notches, and light-transmitting films are detachably fixed to the tops of the supporting strips. A funnel is fixed between the two supporting strips, a supporting plate is mounted below the funnel, a spring is mounted between the bottom of the supporting plate and the bottom plate, and a positioning groove matched with a test tube is formed in the supporting plate. According to the utility model, spore in-situ collection can be carried out on a specific leaf of a certain golden dog, so that uniform maturity of spores is ensured, mixing is avoided, and the golden dog cannot be damaged.

The invention discloses a children growth promoting preparation, which belongs to the technical field of traditional Chinese medicine preparations and comprises fructus alpiniae oxyphyllae, radix pseudostellariae, dried ginger, angelica sinensis, fructus amomi, radix inulae inula, pericarpium citri reticulatae, serissa serissoide, panax japonicus, rhizoma cibotii, radix scrophulariae and cattle bone powder. The preparation integrates the traditional Chinese medicine theory of tonifying spleen and kidney and regulating qi and blood, wherein the fructus alpiniae oxyphyllae, radix pseudostellariae, dried ginger and the like can tonify spleen and promote appetite, significantly improve the digestive function and improve the absorption rate of nutrient substances; the Tibet inula root and the fructus amomi regulate middle-jiao qi movement, relieve abdominal distension and qi stagnation and further optimize the absorption state; the panax japonicus, the rhizoma cibotii and the cattle bone powder directly act on a skeletal system to tonify kidney and strengthen bone, promote cartilage cell proliferation and bone longitudinal growth, and effectively improve height and growth speed. The formula gives consideration to intervention on growth disorder problems such as precocious puberty and the like, and through two-way regulation of nourishing yin and purging fire and tonifying kidney and replenishing essence, advanced bone age is delayed, and precious time is won for height increase.

The application discloses a common culture medium for differentiation, proliferation and rooting culture of Cibotium barometz and a tissue culture method, and belongs to the technical field of tissue culture of Cibotium barometz. The common culture medium comprises: modified 1 / 2MS+6-BA 0.1 mg / L+GA3 0.1 mg / L+IBA 0.1 mg / L; wherein the modified 1 / 2MS contains KNO3 3640 mg / L and NH4NO3 9500 mg / L. The tissue culture method comprises the following steps: after Cibotium barometz is germinated, Cibotium barometz seedlings are obtained through differentiation, proliferation and rooting culture. The application can improve the differentiation rate of Cibotium barometz, and makes the tissue culture of Cibotium barometz simpler, more reliable and more economical.

The invention relates to an application of a traditional Chinese medicine composition in preparation of a medicine for gout, which is characterized in that the traditional Chinese medicine composition is prepared from rehmannia root, cistanche, rhizoma cibotii, glossy privet fruit, epimedium, suberect spatholobus stem, radish seed, rhizoma drynariae and radix achyranthis bidentatae. The composition disclosed by the invention can be used for remarkably improving inflammatory response of a gout mouse model; the arthrocele of a mouse gout model caused by MSU and the level of IL-18 and caspase-1 in joint tissues can be obviously reduced. The total cell number, the percentage of neutrophil and the levels of IL-18 and IL-8 in mouse air sac lavage fluid are remarkably reduced, and it is prompted that the traditional Chinese medicine composition can remarkably inhibit the inflammatory reaction induced by MSU and has a good treatment effect on arthritis caused by gout.

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine preparation for treating osteosarcoma and a preparation process thereof. The traditional Chinese medicine composition is prepared from oroxylum indicum, radix clematidis, scorpio, hedgehog hide, asarum, hot rhizoma cibotii, obscured homalomena rhizome, pseudo-ginseng, rhizoma drynariae, medulla stachyuri and kadsura pepper stem. Systematic experiments prove that the traditional Chinese medicine composition has a remarkable inhibition effect on osteosarcoma cells; in the aspect of clinical application, the prognosis condition of a patient is effectively improved through cooperation with operation and chemotherapy; and in terms of preparation performance, by optimizing the ratio of auxiliary materials and a preparation process, the obtained product is stable in quality and has important clinical application value and market potential.

The present invention relates to the technical field of plant cultivation, and particularly relates to a method for cultivating Cibotium barometz spores. A method for cultivating Cibotium barometz spores comprises the following steps: S1: After the substrate is formulated and pre-moistened with water, it is subjected to high-pressure sterilization treatment; S2: The sterilized substrate is spread into a seedling tray as the spore sowing substrate; S3: The Cibotium barometz spores are made into a suspension and sprayed onto the seedling substrate in S2, and daily management is carried out until the spores germinate; S4: Once the antheridia and archegonia are formed, it is soaked thoroughly with water to facilitate the combination of sperm and eggs to form a sporophyte; S5: According to the growth situation of the seedlings, potting is carried out until the seedling height is about 6 cm - 10 cm, and when tubers are formed at the roots, the seedling cultivation is completed and transferred to under-forest cultivation; this method has the advantages of fast spore germination, neat emergence, strong seedling condition and high emergence rate.

The invention provides a preparation method and application of a traditional Chinese medicine composition for haemorrhoids and anal fissure, the preparation method comprises the following steps: preparing raw materials: 300g of acacia catechu, 100g of borneol, 100g of cibotium barometz, 100g of angelica dahurica, 100g of safflower, 50g of dragon's blood and 300g of Zhang dan, respectively grinding the raw materials into powder during preparation, uniformly mixing, taking 1000g of vaseline and 800g of wool fat, and uniformly mixing the powder with the vaseline and the wool fat to prepare the traditional Chinese medicine composition for haemorrhoids and anal fissure. And heating, filtering, cooling to 55 DEG C, adding the powder of the raw materials, and stirring until the powder is semi-solidified. The formula disclosed by the invention is simple to prepare, few in adverse reaction after being used, good in treatment effect on red and swollen, pain, swelling, bleeding, prolapse, anal fissure, haemorrhoids, internal hemorrhoids, external hemorrhoids and mixed hemorrhoids, and capable of promoting recovery of local wounds of the anus, relieving inflammation, promoting blood circulation, accelerating the blood coagulation process, reducing bleeding, reducing local hyperemia and vein blood vessel pressure; and moreover, by inhibiting the release of an inflammatory mediator, local scorching hot stabbing pain and falling swelling feeling can be relieved.

The invention discloses an SSR molecular marker primer development method of cibotium barometz, a primer and application of the primer, and belongs to the technical field of biology. According to the development method of the SSR molecular marker primer of the Poyang Lake Dog, disclosed by the invention, the SSR molecular marker aiming at the Poyang Lake Dog wild Poyang Lake Dog population is developed, and the developed molecular marker is utilized to carry out genetic diversity analysis on the Poyang Lake Dog wild Poyang Lake Dog population, so that the genetic diversity of the Poyang Lake Dog population is revealed; and theoretical guidance is provided for nursing practice and germplasm resource identification of the golden dog in the Poyang Lake basin. Compared with an existing molecular marker technology, the method has the synergistic advantages of co-dominant heredity, high site specificity, simplicity, convenience and high efficiency in operation and stable and reliable result; the codominant characteristic of the primer can provide complete allele information, the bottleneck that dominant marker genetic information is incomplete is effectively overcome, meanwhile, based on the technical path of specific primers and standard PCR, the repeatability and efficiency of detection are remarkably improved, and a solid foundation is laid for precise genotyping and high-throughput application.

The invention discloses a pharmaceutical composition for prolonging life and preserving health. The traditional Chinese medicine composition comprises the following components: prepared rehmannia root, polygonum multiflorum, herba epimedii, male silkworm moth, schisandra chinensis, tribulus terrestris, dried ginger, angelica sinensis, bighead atractylodes rhizome, liquorice, cistanche, rhizoma cibotii, pseudo-ginseng, winter wheat, dendrobe, pericarpium citri reticulatae, glossy privet fruit, poria cocos, eucommia ulmoides, ligusticum chuanxiong hort, rhizoma alismatis, cudweed, lily, tendril-leaved fritillary bulb, rhizoma anemarrhenae, spina date seed, coix seed, medicated leaven, astragalus membranaceus, morinda officinalis, endothelium corneum gigeriae galli, musk, raspberry, codonopsis pilosula, phellinus igniarius, gecko, scorpion and ground beetle. A femtosecond wall breaking technology is combined with an ancient processing method, and medicated leaven is used for double fermentation, so that the medicine effect is improved by 8-10 times; the antioxidant capacity of the body can be improved, free radicals, immune functions and nerve protection functions are eliminated, and senescence is delayed; the medicine can treat old and chronic diseases. The preparation forms comprise decoction, oral liquid, pills and medicinal liquor. The composition can significantly improve the activity of antioxidant enzymes such as SOD, CAT and the like, reduce the MDA content and improve the cognitive and athletic ability.

This invention discloses a method for direct seeding propagation of *Cibotium barometz* using native soil without sterilization, belonging to the field of plant propagation and seedling technology. This invention achieves ecological safety, low cost, and high efficiency in *Cibotium barometz* seedling cultivation, completely abandoning the existing method of sterilized substrate + tissue culture. It pioneers a zero-sterilization direct seeding technology using native soil, resolving the core contradiction between the harmful effects of sterilizing agent residues and the destruction of beneficial microorganisms. By utilizing locally sourced materials and natural forest light, the cost per seedling is reduced to ≤0.25 yuan, significantly lower than traditional techniques (≥3 yuan / seedling). Simultaneously, the seedling cultivation cycle is shortened by more than 30%. Relying on the natural microbial community of the native soil and the natural environment under the forest, the seedlings exhibit strong resistance and a high transplant survival rate (≥75%), achieving efficient integration of seedling cultivation and planting.

The invention belongs to the technical field of artificial breeding of plants, and discloses an artificial rapid breeding and efficient cultivation method of cibotium barometz, which comprises the following steps: spore disinfection and germination: collecting mature spores of cibotium barometz, performing surface disinfection, inoculating into a first culture medium, and performing germination culture to obtain gametophytes; seedling induction and rooting: transferring the gametophyte into a second culture medium, inducing the gametophyte to develop into a seedling and root, and culturing until the seedling has a robust root system; acclimatization and transplanting: transplanting the rooted seedlings into an acclimatization matrix, performing acclimatization culture in an acclimatization environment with the humidity controlled to be 88-92% and the temperature controlled to be 18-22 DEG C, and then transplanting the seedlings into natural soil. According to the method, the pollution rate is reduced from 50% to 15%, and the disinfection success rate of the cibotium barometz spores is greatly improved; growth hormone is added, so that the germination period of spores and the rooting period of seedlings are shortened; the survival rate of the seedlings can reach 80%, and the success rate of seedling hardening is increased.