Iron-reducing comamonas and application thereof

A technology of Comamonas and microbial strains, applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of inability to use organic matter, not yet found Comamonas, and high environmental requirements

Inactive Publication Date: 2009-11-25
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These strains still have the following defects: 1) Most strains can only survive in a strict anaerobic environment, which is not conducive to large-scale production and practical application; 2) The electron donor utilization spectrum of the strains is narrow, and only acetic acid, lactic acid, etc. Small-molecular-w

Method used

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  • Iron-reducing comamonas and application thereof
  • Iron-reducing comamonas and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: Separation, purification of bacterial classification

[0024] Take 5g ancient forest soil sample in 100mL liquid medium, the medium is composed of (g / L): AQDS (anthraquinone-2,6-disulfonic acid) 0.0412g, sodium acetate 1.36g, NaHCO 3 2.5g, NH 4 Cl 0.25g, NaH 2 PO 4 2H 2 O 0.678g, KCl 0.1g, vitamin solution 10.0mL (each liter of vitamin solution contains: biotin 2.0mg, folic acid 2.0mg, vitamin B 6 10.0mg, riboflavin 5.0mg, vitamin B 1 50mg, Niacin 5.0mg, Pantothenic Acid 5.0mg, Vitamin B 12 0.1mg, lipoic acid 5.0mg), trace element solution 10.0mL (each liter of trace element solution contains: nitrilotriacetic acid 1.5g, MgSO 4 ·7H 2 O 3.0g, MnSO 4 ·H 2 O 0.5g, NaCl 1.0g, FeSO 4 ·7H 2 O 0.1g, CoCl 2 ·6H 2 O 0.1g, CaCl 2 0.1g, ZnSO4 ·7H 2 O 0.1g, CuSO 4 ·5H 2 O 0.01g, AlKSO 4 12H 2 O 0.01g, H 3 BO 3 0.01g, Na 2 m 0 o 4 0.01g, NiCl 2 ·6H 2 O 0.024g), pH 5.0-5.5. Before inoculation, the culture medium is filled with high-purity m...

Embodiment 2

[0025] Embodiment 2: the identification of strain

[0026] (1) Determination of physiological and biochemical properties:

[0027] Table 1. Physiological and biochemical characteristics of CY01 strain

[0028]

[0029] Note: + indicates that it can be used; - indicates that it cannot be used

[0030] (2) Molecular biological identification

[0031] SDS-proteinase K, chloroform-isoamyl alcohol (volume ratio 24:1) extraction and 0.6 volume isopropanol precipitation were used to extract the total bacterial DNA. Bacterial 16S rDNA general primers F8 and R1542 were used to amplify bacterial 16S rDNA, and obvious bands appeared around 1500 bp. The PCR amplification product was recovered and sequenced. The obtained DNA sequence was input into GenBank, and the database was checked by Blastn program. All sequences were compared and analyzed. Combined with the results of the above physiological and biochemical characteristics and 16S rDNA sequence, the strain should belong to the...

Embodiment 3

[0032] Example 3: Identification of reducing activity

[0033] The electron donor is glucose and the electron acceptor is ferrihydrite

[0034] Medium formula: 10g of hydrous iron ore per liter of deionized water, NaHCO 3 2.5g, NH 4 Cl0.25g, NaH 2 PO 4 2H 2 O 0.678g, KCl 0.1g, glucose 0.9008g, each 10.0mL of vitamin solution and trace element solution (the formula of vitamin solution and trace element solution is the same as in Example 1), pH 5.0~5.5; When the glucose is separated from other components, the glucose and other components are mixed after sterilization, and then filled with high-purity mixed gas (N 2 / CO 2 =80 / 20)≥1 hour, inoculate the C. ferrireducens CY01 bacterial suspension to make the number of bacteria reach 10 8 / mL, static anaerobic culture at 30°C, and a control without bacteria was set at the same time. To observe the color change of the culture solution, take 4 mL of the culture solution in 16 mL of 0.5M HCl solution (M represents the number of...

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Abstract

The invention discloses an iron-reducing comamonas and an application thereof. The inventor screens the iron-reducing comamonas with the humus reduction activity and the iron reduction activity by a great deal of experiments. The bacteria can reduce the ferric iron such as ironic citrate, iron hydroxide, ferrihydrite and goethite; and reduce the humus and the humic model AQDS (anthraquinone-2, 6-disulfonic acid). The iron-reducing comamonas is the facultative anaerobe, has wide electro donor spectrum. The electro donors which can be oxidized by the bacteria in the anaerobic condition are glycerine, glucose and sucrose.

Description

[0001] The present invention is a divisional application, the filing date of the parent case is June 1, 2007, the application number is 200710028384.8, and the invention name is "Comamonas iron-reducing bacteria and its application" technical field [0002] The invention relates to the technical field of new strain screening, in particular to Comamonas iron-reducing bacteria and its application. Background technique [0003] Fe(III) and humus are two components that are abundantly present in soil. Fe(III) mainly exists in the form of insoluble iron oxide, with an average content of about 5.6% in the earth's crust, and is the electron acceptor with the highest natural abundance in anaerobic soils. Humus is a quinone-rich, heterogeneous natural organic mixture. It is the main body of soil organic matter, accounting for about 60-80% of the total organic carbon. It plays an important role in soil fertility, sustainable agricultural development, and environmental protection. eff...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
Inventor 周顺桂武春媛王弋博李芳柏
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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