Method for degrading polyethylene terephathalate

A technology of diethyl terephthalate and diethyl phthalate, which is applied in the field of degradation of diethyl terephthalate, can solve the problems of poor water solubility and difficult catalytic degradation, and achieves fast degradation speed and degradation effect. Good, targeted effect

Inactive Publication Date: 2012-08-01
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for water-insoluble substances containing phenylcyclic esters, due to their poor water solubility, it often requires the combined action of multiple microorganisms and multiple biological enzymes to completely degrade them into carbon dioxide and water, so it is difficult to be catalyzed by a single microorganism.
Within the scope of the applicant's search, there is no report on the complete degradation of diethyl terephthalate (DTP) by a single strain of Comamonas testosteroni

Method used

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  • Method for degrading polyethylene terephathalate
  • Method for degrading polyethylene terephathalate
  • Method for degrading polyethylene terephathalate

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Embodiment 1

[0035] 1g / L DTP was used as the sole carbon source, and Comamonas testosteroni was used to degrade DTP.

[0036]The Comamonas testosteroni strains obtained by separation were cultivated for 12 hours at 30°C in a slant solid medium (compositions as mentioned above, the same below), and stored in the refrigerator for subsequent use; according to the composition of the liquid medium (compositions as mentioned above Said, the same below), the preparation DTP concentration is 5 parts of the substrate solution of 1g / L, every part of 50ml. Put them in five 150ml Erlenmeyer flasks respectively, bandage and sterilize (121°C, 25min), and cool down for later use; Shake the culture at 30°C and 200r / min to make it catalyze the degradation of the substrate DTP; start timing at zero time when there is no reaction, take out a bottle of degradation solution every 12h, inactivate it in a water bath at 95°C for 5min, add 10ml n-hexane after cooling Alkanes, shake fully, extract the undissolved ...

Embodiment 2

[0041] 3g / L DTP was used as the sole carbon source, and Comamonas testosteroni was used to degrade DTP.

[0042] Cultivate the isolated strains at 34°C in a slant solid medium for 15 hours, and store them in the refrigerator for later use; prepare 7 substrate solutions with a DTP concentration of 3g / L according to the composition of the liquid medium, each 50ml. Put them in seven 150ml Erlenmeyer flasks, wrap and sterilize (121°C, 25min), and cool down for later use; transfer the bacteria in the slant culture medium to the substrate solution to be degraded to prepare the degradation solution, and Shake culture at 34°C and 200r / min to make it catalyze the degradation of the substrate DTP; start timing from the unreacted zero hour, take out a bottle of degradation solution every 12h, inactivate it in a water bath at 95°C for 5min, add 10ml n-hexane after cooling Alkanes, shake fully, extract the undissolved DTP, after standing for defoaming, take the liquid in the n-hexane layer...

Embodiment 3

[0047] Using 5g / L DTP as the sole carbon source, Comamonas testosteroni was used to degrade DTP.

[0048] Cultivate the isolated Comamonas testosteroni strains at 37°C for 18 hours in a slant solid medium, and store them in the refrigerator for later use; prepare 9 parts of a substrate solution with a DTP concentration of 5g / L according to the composition of the liquid medium, 50ml per serving. Put them in nine 150ml Erlenmeyer flasks, bandage and sterilize (121°C, 25min), and cool down for later use; transfer the strains in the slant culture medium to the substrate solution to be degraded to prepare the degradation solution, and Shake culture at 37°C and 200r / min to make it catalyze the degradation of the substrate DTP; start timing from the unreacted zero hour, take out a bottle of degradation solution every 12h, inactivate it in a water bath at 95°C for 5min, add 10ml n-hexane after cooling Alkanes, shake fully, extract the undissolved DTP, after static defoaming, take the...

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Abstract

The invention discloses a method for degrading polyethylene terephathalate. The method mainly includes the following steps: 1, strain culture: comamonas testoseroni strain is cultured for 12-18h in a solid medium with inclined surface at 30-37 DEG C; 2, substrate solution preparation: 5-9 parts of substrate solution with the concentration of 1-5g / L are prepared, each part is 50ml and is respectively placed into a 150ml conical flask, and packing up and sterilizing for 25min are carried out at 121 DEG C; 3, substrate degradation: the strain in the step 1 is inoculated into the substrate solution in the step 2, so as to obtain degradation liquor, and shake culture is carried out at the rotating speed of 200r / min and at the temperature of 30-37 DEG C, so as to catalyze degradation on the substrate; 4, substrate degradation ratio detection: 400-190nm wavelength scanning is adopted, and variation of substrate content is judged according to absorption peak at 240nm; 5, substrate degradationproduct detection: 400-190nm wavelength scanning is adopted, and variation of degradation intermediate product content is judged.

Description

technical field [0001] The invention relates to microbial whole cell catalytic degradation technology, in particular to a degradation method of diethyl terephthalate. Background technique [0002] Substances containing benzene rings are widely used in the chemical industry, so the amount of industrial waste formed is large, and benzene rings have certain toxic effects on the environment, and even have carcinogenic and teratogenic effects on organisms. Therefore, benzene rings The degradation of substances is a hot topic of research. Biodegradation is a commonly used method for the degradation of pollutants. Benzene rings have certain biodegradability. Therefore, there are many studies on microbial degradation of substances containing benzene rings. [0003] In the degradation of substances containing benzene rings, the degradation of carboxylic acids containing benzene rings has been studied more. This kind of substance has a certain water solubility, and the microorganism...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A62D3/02A62D101/28
Inventor 张健飞张卫玲巩继贤李政李秋瑾
Owner TIANJIN POLYTECHNIC UNIV
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