Oncomelania killing bacteria and application thereof

A technology for killing bacteria and molluscs, applied in the field of microorganisms, can solve the problems of irreversible damage to the environment, and achieve the effects of low cost, environmental friendliness, and simple preparation methods

Inactive Publication Date: 2014-02-26
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main molluscicides are chemical drugs, which are likely to cause irreversible damage to the environment

Method used

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  • Oncomelania killing bacteria and application thereof
  • Oncomelania killing bacteria and application thereof
  • Oncomelania killing bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Screening of the mollusc-killing bacteria CCTCC M 2013442 strain:

[0016] 1. Preparation of culture medium:

[0017] 1) YEB liquid medium: beef extract 5.0g / L, yeast extract 1.0g / L, peptone 5.0g / L, MgSO 4 ·7H 2 O is 4g / L, sucrose is 5.0g / L, pH7.0~7.5;

[0018] 2) YEB solid medium: add 15g / L agar to the above YEB liquid medium.

[0019] 2. Screening steps:

[0020] 1) Under sterile conditions, take 8 dying negative Oncomelania snails, soak them in 75% alcohol for 3 minutes, wash them with sterile saline for 3 times, crush and grind the snails, add 2 mL of sterile physiological Saline, to obtain tissue serous;

[0021] 2) Inoculate the tissue slurry from step 1) into 100 mL of YEB liquid medium, culture at 37°C for 12 to 16 hours, and the rotation speed of the culture is 200 rpm to obtain the culture medium;

[0022] 3) Dilute the culture solution in step 2) with sterile saline to 10 by volume -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 and 10 -7 , to obtain the d...

Embodiment 2

[0031] This embodiment is the determination of molluscicidal activity of the molluscicide bacterium CCTCC M 2013442:

[0032] 1. Preparation of molluscicide:

[0033] Inoculate the mollusc-killing bacterium CCTCC M 2013442 into 100 mL of YEB liquid medium, and cultivate it at 37°C for 24 hours at a speed of 200 rpm to obtain seed liquid. Transfer 1 mL of the seed liquid to 100 mL of YEB liquid medium and heat Cultivate for 12 to 16 hours at a rotational speed of 200 rpm to obtain a fermented liquid, which is a biological molluscicide.

[0034] 2. Preparation of molluscicide dilution: the molluscicide is prepared with dechlorinated water to make a solution with a volume fraction of 10%, which is the molluscicide dilution.

[0035] 3. Determination of molluscicidal activity:

[0036] After the snails collected in the field were cultured at 25°C for 3 days, the snails with good vigor were selected and rinsed with dechlorinated water for 3 times, and the snails were divided into...

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Abstract

The invention discloses an oncomelania killing bacteria. The biological collection number of the oncomelania killing bacteria (comamonas sp.X3) is CCTCC M 2013442; and the oncomelania killing bacteria CCTCC M 2013442 is comamonas bacteria which is screened from moribund negative oncomelania and can quickly and effectively kill oncomelania. The bacteria provided by the invention is capable of killing oncomelania and can be directly used as a biological molluscacide; by comparing the biological molluscacide prepared from the oncomelania killing bacteria CCTCC M 2013442 with the traditional chemical molluscacide, the preparation method is simple and environment-friendly while the cost is low.

Description

technical field [0001] The present invention relates to a microorganism, in particular, the present invention relates to a mollusc-killing bacterium and application thereof. Background technique [0002] Oncomelania is the only intermediate host of schistosomiasis, and its reproduction and distribution are closely related to the prevalence and spread of schistosomiasis. Therefore, suppressing snails and killing snails is considered an important way to prevent the spread of schistosomiasis. At present, the main molluscicides are chemical drugs, which are likely to cause irreversible damage to the environment. In recent years, the microbial community structure in animals has been widely concerned and studied. Scholars at home and abroad have found through research that there are a large number of microorganisms in animals. These microorganisms play an important role in the growth, development, health, etc. of animals. Therefore, the present invention will The direction of res...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P9/00C12R1/01
Inventor 叶爱华王梅方孙启祥张倩张玉琼韦朝领
Owner ANHUI AGRICULTURAL UNIVERSITY
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