Mixed bacterium for efficiently degrading atrazine and fermental culture method

Abstract

The invention relates to burkholderia caribensis and comamonas for efficiently degrading atrazine. A mixed fermental culture medium formula and a culture method for high-density cell culture are obtained through response surface optimization design, and specially, a mixed bacterium for efficiently degrading atrazine and a fermental culture method are provided. According to the mixed bacterium, the burkholderia caribensis and the comamonas for efficiently degrading atrazine are utilized; a formula of a fermental culture medium for high-density cell culture is obtained through response surface optimization design; the formula comprises 6.72g of cane sugar, 6.75g of glucose, 6.34g of bean pulp, 0.6g of dipotassium phosphate, 2.5g of monopotassium phosphate, 0.3g of magnesium sulfate heptahydrate, 0.4g of sodium chloride, 3g of yeast powder and 1L of distilled water; the pH is 7.0-7.5. The cultured mixed bacterial liquid is adopted for soil remediation tests, and 200mg/L of atrazine can be completely degraded within 7 days. A high-density fermental culture formula and method are provided for development of the mixed bacteria.

Description

technical field [0001] The invention relates to Burkholderia and Comamonas bacteria capable of efficiently degrading atrazine, and obtains a mixed fermentation medium formula and a culture method for high-density cell culture through response surface optimization design, specifically a A mixed bacterium for efficiently degrading atrazine and a fermentation culture method for the mixed bacterium. Background technique [0002] Atrazine, also known as atrazine (atrazine, atrazine), is characterized by containing benzene ring, stable structure, low water solubility, small adsorption coefficient and long residual period. At present, due to long-term and extensive use, it has caused serious pollution to the environment. Therefore, it is of great significance to study the degradation of atrazine. [0003] In previous studies, it was found that there are a large number of microorganisms with strong adaptability in nature. They can completely mineralize or degrade various pesticide...

AI Technical Summary

Problems solved by technology

[0005] The research on the degradation of atrazine by using microbial preparations has made some progress, but most of the

Images