Aquatic comamonas and culture method and application thereof

Comamonas bacteria and medium technology, applied in the field of microorganisms and biodegradation, can solve the problems of high cost and secondary pollution

Active Publication Date: 2016-03-23
TIANJIN CARING TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chemical method needs to add a large amount of chemicals, the cost is high, and it is easy to cause secondary pollution

Method used

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  • Aquatic comamonas and culture method and application thereof
  • Aquatic comamonas and culture method and application thereof
  • Aquatic comamonas and culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Isolation and identification of Comamonas aquaticus KY-02 of embodiment 1

[0022] The Comamonas aquaticus KY-02 of the present invention is screened out from the activated sludge of the pulping and papermaking sewage plant, and its colony is in the form of a flake lawn on the culture plate, the lawn is flat and opaque, very large, white, with fluff on the surface Dry, branched and cracked at the edge, thick rod-shaped monomer, Gram stain negative, and has a strong ability to degrade soluble cellulose.

[0023] Based on the bacterial morphology, physiological and biochemical characteristics and 16SrDNA gene sequence (as shown in SEQ ID NO.1), the bacterial strain was identified as Comamonas aquaticus and named KY-02. The strain was preserved on December 11, 2015 in the General Microbiology Center of China Committee for Culture Collection of Microorganisms (CGMCC for short, address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, ...

Embodiment 2

[0024] The performance test of the cellulose degradation of embodiment 2 Comamonas aquaticus KY-02

[0025] Experiment 1, described Comamonas aquaticus KY-02 is inserted in culture medium, wherein the composition of culture medium is: (NH 4 ) 2 SO 4 2g, K 2 HPO40.5g, NaCl0.5g, MgSO 4 0.5g, KNO 3 1g, 0.5-1.5g cellulose sulfonate, 1000ml distilled water, PH7.2 culture for 2 days until the bacterial concentration is about 10 5 -10 6 / ml; With 5% inoculum size, the aerobic section effluent of Tianjin Xinghe Paper Industry Paper Wastewater Treatment Plant and Ninghe Paper Industry Paper Waste Water Treatment Plant is the experimental object, and every sewage 100ml is packed in the 500ml triangular flask (numbering For 1 and 2), seal the mouth of the Erlenmeyer flask with a silica gel stopper, and incubate at 180r / min, 30°C for 2 days. Samples were taken separately, and after centrifugation, the cellulose content in the supernatant was measured by ultraviolet spectrophotometr...

Embodiment approach

[0027] Implementation method: the Comamonas aquaticus KY-02 is inserted into the culture medium, wherein the composition of the culture medium is: (NH 4 ) 2 SO 4 2g, K 2 HPO40.5g, NaCl0.5g, MgSO 4 0.5g, KNO 3 1g, 0.5-1.5g cellulose sulfonate, 1000ml distilled water, PH7.2 culture for 2 days until the bacterial concentration is about 10 5 -10 6 / ml; With 10% inoculum amount, the aerobic section effluent of Tianjin Xinghe Paper Industry Paper Wastewater Treatment Plant and Ninghe Paper Industry Paper Waste Water Treatment Plant is the experimental object, and every sewage 100ml is packed in the 500ml triangular flask (numbering For 3 and 4), seal the mouth of the Erlenmeyer flask with a silica gel stopper, and incubate at 180 r / min at 30° C. for 2 days. Samples were taken separately, and after centrifugation, the cellulose content in the supernatant was measured by ultraviolet spectrophotometry. The results are shown in Table 1.

[0028] Experiment 3, the medium content ...

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Abstract

The invention provides aquatic comamonas and a culture method and application thereof. The aquatic comamonas is KY-02, and the preservation number is CGMCC No.11866. The aquatic comamonas KY-02 can degrade cellulose with the content of 1500 mg/L in sewage, so that the COD of sewage is lowered, the degradation onset time is short, and the degradation rate can reach about 15% on the second day. Compared with physical and chemical methods, the aquatic comamonas has the advantages of being low in cost, free of secondary pollution and the like, and has the wide application prospect and the good economic value in the field of papermaking sewage treatment.

Description

technical field [0001] The invention relates to the field of microorganisms and biodegradation, in particular to Comamonas aquaticus and its cultivation method and application. Background technique [0002] The paper industry has a large amount of sewage discharge, and the sewage contains a large amount of cellulose, cellulose, inorganic alkali, resin, tannin, protein, etc., resulting in deep color, high alkalinity, high content of refractory organic matter, and high oxygen consumption. , which is a typical comprehensive high-concentration organic sewage. my country's paper industry pollution is particularly serious. The pollution of the water environment by the paper industry has seriously threatened environmental safety and human health, and has attracted widespread attention worldwide. Solving the pollution of papermaking wastewater has become a top priority. [0003] In recent decades, the biodegradation of pollutants has been studied in considerable detail and many re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F103/28
CPCC02F3/34C02F2103/28C12N1/205C12R2001/01
Inventor 王晓阳李玉庆张建薛二军韩超
Owner TIANJIN CARING TECH DEV
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