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Composite microbial agent capable of degrading agricultural wastes

A technology of agricultural waste and compound microbial inoculants, applied in the field of microorganisms, can solve the problems of high energy consumption by physical methods, weak cold resistance, secondary pollution by chemical methods, etc., achieve significant economic and social benefits, avoid soil compaction, animal good palatability

Active Publication Date: 2017-06-13
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of comprehensive utilization of straw has been studied for decades, but there are still many problems. For example, the physical method has the challenge of high energy consumption, the chemical method will cause secondary pollution, and the biological method is environmentally friendly but has low efficiency. The problem
In order to improve the efficiency of straw degradation, scientists have conducted a lot of research on straw-degrading microbial agents, and obtained a variety of strains that degrade straw cellulose and lignin. However, the strains obtained in the current research have low enzyme activity, poor stability, and easy degradation. , especially weak cold tolerance did not significantly improve the effect of returning to the field, so the breeding of strains that can withstand low temperature and efficiently degrade straw is of great significance for the further effective use of crop straw

Method used

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  • Composite microbial agent capable of degrading agricultural wastes
  • Composite microbial agent capable of degrading agricultural wastes
  • Composite microbial agent capable of degrading agricultural wastes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: preparation process of composite bacterial agent

[0016] A kind of microbial compound bacterial agent, is made of Enterobacteriaceae ( Enterobacter sp. ), Comamonas ( Comamonas serinivorans ), Rhodococcus ( Rhodococcus sp. ) and Bacillus ( Bacillus ligniniphilus )composition.

[0017] The mass percentage of each component is: Enterobacter 20%-30%, Comamonas 30%-40%, Rhodococcus 10%-20%, Bacillus 20%-40%.

[0018] Wherein, enterobacteria described in this embodiment ( Enterobacter sp. )YC105C, was deposited in China General Microorganism Culture Collection Center on March 20, 2014, with the preservation number CGMCC No.8941, and has applied for a patent (application number 201510010882.4).

[0019] Comamonas ( Comamonas serinivorans ) C35, which was deposited in the Japan Culture Collection of Microorganisms on April 15, 2014, with a preservation number of JCM No. 18194, and its isolation, screening and identification process is detailed in ...

Embodiment 2

[0025] Example 2: Composite microbial agent for degrading corn stalks

[0026] Inoculate 0.5 g of the compound microbial agent mixed with 25% Enterobacter, 35% Comamonas, 20% Rhodococcus, and 20% Bacillus in the corn stalk degradation medium, and culture it statically at 25°C for 30 days , detect the quality of the remaining straw, the content of reducing sugar and crude protein in the medium, and culture under the same conditions after inoculation with 0.5 g of pure Rhodococcus inoculum as a comparison.

[0027] The components of the corn stalk degradation medium are: K 2 HPO 4 1.0 g, MgSO 4 •7H 2 O 0.3g, NaCl 0.1g, FeCl 3 0.01g, CaCl 2 0.1 g, 5 g corn stalks, 1000 mL distilled water, natural pH.

[0028] The method for measuring the weight loss rate of straw: carefully discard the supernatant of the culture solution, rinse the remaining straw several times with dilute hydrochloric acid, then rinse repeatedly with sterile water until neutral, filter, dry at 60°C unt...

Embodiment 3

[0034] Embodiment 3: Composite bacteria agent for degrading wheat straw

[0035] 0.7 g of compound microbial agent mixed with 20% Bacillus, 40% Comamonas, 15% Rhodococcus and 25% Bacillus was inoculated into the wheat straw degradation medium, and cultured at 20°C for 30 days. And 0.7g of pure Enterobacteriaceae inoculum was used as a comparison.

[0036] The components of the wheat straw degradation medium are: K 2 HPO 4 1.0 g, MgSO 4 •7H 2 O 0.3g, NaCl 0.1g, FeCl 3 0.01g, CaCl 2 0.1 g, 8 g wheat straw, 1000 mL distilled water, natural pH.

[0037] All the other methods are the same as in Example 2.

[0038] The degradation effect is shown in Table 2. After 30 days of cultivation, the weight loss rate of pure Enterobacteriaceae and compound bacterial agents on corn stalks reached 29% and 46%, respectively. The degradation efficiency of the composite bacterial agent was significantly higher than that of the single bacteria Enterobacter, and the reducing sugar and c...

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Abstract

The invention discloses a composite microbial agent capable of degrading agricultural wastes and belongs to the technical field of microbes. The active ingredients of the agent refer to the following strains separated from environments such as termite guts, composted straws and sediments of South China Sea: enterobacterium (Enterobacter sp.)YC105C, comamonas (Comamonas serinivorans) C35, rhodococcus (Rhodococcus sp.) L17 and bacillus (Bacillus ligniniphilus) L1. The composite microbial agent contains the concentrated bacterium powder of the following components in percentage by mass: 20-30% of enterobacterium, 30-40% of comamonas, 10-20% of rhodococcus and 20-40% of bacillus. The composite microbial agent is capable of degrading the agricultural wastes such as wheat straws, maize straws and green soybean straws, the quantity of the straws returned to the field is increased, and the cultivated land quality is improved, so that the growth of crops is promoted.

Description

technical field [0001] The invention relates to a composite bacterial agent for degrading agricultural waste, belonging to the technical field of microorganisms. Background technique [0002] China is a large agricultural country. With the continuous improvement of planting and cultivation techniques, grain production has increased year by year, and a large amount of crop straw has also been produced. For quite a long period of time in the past, straw was used as energy by direct combustion. With the development of rural economy and the improvement of firewood structure, the amount of straw used in rural energy consumption is less and less, and more straw is used. become waste. These discarded straws are randomly piled up or burned on the spot, causing serious environmental pollution and a huge waste of resources. Therefore, straw returning to the field has attracted more and more attention. Returning crop straw to the field in situ can not only improve the comprehensive u...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18A23K10/12A23K10/30C05G3/04C05F11/08C12R1/01C12R1/07C05G3/80
CPCC05F11/00C05F11/08C05G3/80C12N1/20Y02P60/87
Inventor 朱道辰张佩佩
Owner JIANGSU UNIV
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