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Degradation bacterium capable of efficiently degrading propyzamide and application thereof

A technology of benzamid and high-efficiency degrading bacteria, which is applied in the field of microorganisms

Inactive Publication Date: 2014-05-28
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, no report on the biodegradation of benzamidrel has been found, so the screening of strains that can efficiently degrade benzamidrel has very good practical significance for the development and utilization of degradable gene resources.

Method used

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  • Degradation bacterium capable of efficiently degrading propyzamide and application thereof
  • Degradation bacterium capable of efficiently degrading propyzamide and application thereof
  • Degradation bacterium capable of efficiently degrading propyzamide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment W1

[0028] Screening of Example W1

[0029] Take 5g of soil samples (collected from Yancheng City) and place them in 100ml enrichment medium containing 50mg / L benzamide (g / L:NH 4 NO 3 1.0g, KH 2 PO 4 0.5g, K 2 HPO 4 1.5g, NaCl1.0, MgSO 4 .7H 2 (00.1g, pH7.0), cultured at 30°C, 180r / min for 5d. Measure the degradation situation of benzamide in the enriched liquid with ultraviolet scanner, after finding that benzamide is degraded, insert in the 100mg / L benzamide medium with 10% inoculum size, continue Enrichment and determination of the degradation, according to this method until the concentration of benzamide propargyl increased to 400mg / L, and passaging 3 times. After gradient dilution, the enriched solution of benzamid was spread on the LB plate containing 200 mg / L of benzamid, and cultured in a 30°C incubator for 2 days. The single bacteria with different bacterial colonies grown on the plate were streaked on the LB plate for purification, and inoculated in the liquid i...

Embodiment 2

[0033] Inoculate a single colony of Comamonas sp.W1 (CCTCC NO: M2013464) in 20ml liquid LB medium (g / L: yeast powder 5.0, tryptone 10.0, NaCl10.0, pH 7.0), culture at 30°C, 180r / min 16h to make OD 600nm = 1.0 seed solution. In the inorganic salt medium (g / L:NH 4 NO 3 1.0g, KH 2 PO 4 0.5g, K 2 HPO 4 1.5g, NaCl1.0, MgSO 4 .7H 2 (20.1g, pH7.0), insert seed solution by 1% inoculum size, cultivate with 30 ℃, 180r / min shaker, take a sample once every 2h, measure the concentration of benzamid, bacterial strain W1 can be in 90% of 100mg / L benzamid was degraded within 8 hours.

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Abstract

The invention belongs to the field of microorganisms, relates to a degradation bacterium W1 for propyzamide, and belongs to comamonas; the degradation bacterium is preserved in China Center for Type Culture Collection on October 11th, 2013, the preservation number is CCTCC NO. M2013464, and the registered number of 16S rRNA in Genebank is 1680505. The bacterium can degrade 90% of 100mg / L of propyzamide within 8 hours. The degradation bacterium is used for degrading herbicide propyzamide, and is used for biological purification of water, soil and agriculture products polluted by the propyzamide.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a benzamidrel-degrading bacterium and an application thereof. Background technique [0002] Propyramide is a systemic conduction-type selective amide herbicide developed by Rohm & Haas in 1969. It interferes with cell mitosis through root absorption and conduction. Applicable crops are small seeds and leguminous crops , peanuts, soybeans, lettuce, certain orchards, turf, and some ornamental plants. It has obvious inhibitory and eradicating effects on annual weeds and some perennial weeds. [0003] Biodegradation is an important transformation process of pesticides in the environment. Microorganisms play an important role in the biodegradation of pesticides because they exist in large numbers in nature and have the characteristics of various types, rapid reproduction, and strong adaptability to the environment. So far, there is no report on the biodegradation of benzamidrel, so the ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C02F3/34B09C1/10C12R1/01A62D101/04A62D101/28C02F101/38
Inventor 王鸣华赵百萍华修德施海燕
Owner NANJING AGRICULTURAL UNIVERSITY
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