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Phenolic extract of duchesnea indica focke, preparation method and application thereof

A phenolic and extractive technology of snakeberry, which is applied in the field of traditional Chinese medicine, can solve the problems that the anti-tumor active components of snakeberry have not yet been obtained, and achieve the effect of improving cellular and humoral immune functions and improving human immunity

Inactive Publication Date: 2008-02-13
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported in the literature that snakeberry has significant antitumor activity, but all the reports are the crude extracts of water extraction or alcohol extraction of snakeberry. So far, no antitumor active components of snakeberry have been obtained.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Take 200g of whole snakeberry grass, add 3L of 80℃ hot water, soak and extract 3 times, 1 hour each time. Remove the medicinal residues by filtration, combine the extracts, slowly pass the extracts through the AB-8 macroporous adsorption resin column, wash away sugar, organic salts, amino acids and other non-effective components with 10L water, and then elute with 6L 90% ethanol, The ethanol eluate was collected, concentrated under reduced pressure to dryness to obtain 11 g of phenolic extract of snakeberry. Measured by spectrophotometry (Scalbert A, Monties B, Jannin, G. Tanins in wood: comparison of different estimation methods. Journal of Agricultural and Food Chemistry 1989, 37: 1324-1329.), the total amount of phenols contained in this extract In terms of epicatechin, it is 46.1%.

Embodiment 2

[0018] Take 150g of snakeberry whole grass, add 2.5L of water, make it soaked in the medicinal surface, soak and extract 3 times, 1 hour each time. Remove the dregs by filtration, combine the extracts, slowly pass the extracts through the AB-8 macroporous adsorption resin column, wash away non-effective ingredients such as sugar, organic salts, amino acids, etc. with 10L 0.1% hydrochloric acid aqueous solution, and then use 6L 95 % ethanol for elution, the ethanol eluate was collected, concentrated under reduced pressure to dryness to obtain 9.6 g of phenolic extract of snakeberry. Measured by spectrophotometry (Scalbert A, Monties B, Jannin, G. Tanins in wood: comparison of different estimation methods. Journal of Agricultural and Food Chemistry 1989, 37: 1324-1329.), the total phenolic content in this extract The amount is 41.9% in terms of epicatechin.

Embodiment 3

[0020] Take 130g whole snakeberry grass, add 2L of 80% ethanol to soak and extract 3 times, 1 hour each time. Remove the dregs by filtration, combine the extracts, concentrate the extracts under reduced pressure to remove ethanol, add 5L of water to dilute, pass the filtrate through a D3520 macroporous adsorption resin column, and wash with 10L of water to remove non-effective ingredients such as sugar, organic salts, amino acids, etc. It was then eluted with 6L of 90% ethanol, and the ethanol eluate was collected and concentrated under reduced pressure to dryness to obtain 5.2 g of phenolic extract of snakeberry. Measured by spectrophotometry (Scalbert A, Monties B, Jannin, G. Tanins in wood: comparison of different estimation methods. Journal of Agricultural and Food Chemistry 1989, 37: 1324-1329.), the total amount of phenols contained in this extract In terms of epicatechin, it is 40.2%.

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Abstract

The present invention discloses an Indian strawberry phenol extraction extracted from an Indian strawberry whole grass, which also relates to the preparation method of the extraction and the function of the extraction. The preparation method of the Indian strawberry phenol extraction is as following, which comprises soaking and extraction of the Indian strawberry by water, ethanol, or a mixed solution with water and ethanol in a certain ratio; vacuum concentration of the Indian strawberry extraction liquid; then the ethanol is removed from the extraction liquid and passed through a malodorous resin column; the liquid is eluted by the ethanol after elution of the non affection component with the water; the ethanol elution liquid and vacuum concentration are collected, therefore the Indian strawberry phenol extraction is obtained. The Indian strawberry phenol extraction of the present invention possesses functions of prohibiting the generation and growth of various kinds of tumor cells. Therefore the present invention is applicable for treatment of a plurality of cancer diseases comprising the lung cancer, the liver cancer, the nasopharynx cancer, the gastric cancer, the cervical cancer, the endometrium cancer and the ovarian cancer, etc. In addition, the Indian strawberry phenol extraction in the present invention has active function of improving the immune ability in the organism.

Description

technical field [0001] The invention relates to an extract of active ingredients of traditional Chinese medicine, in particular to a phenolic extract extracted from the plant snakeberry, and also relates to a preparation method of the extract and an application of the extract, belonging to the field of traditional Chinese medicine. Background technique [0002] Snakeberry [Drchesnea indica (Andr) Focke] is a plant of the genus Snakeberry in the family Rosaceae, also known as three winds, ground berries, a little red, snake grass, wild bayberry, etc., and is a commonly used herbal medicine among the people. Snakeberry uses the whole herb as medicine, which has the effects of clearing heat and detoxifying, reducing swelling and dissipating blood stasis, astringent to stop bleeding, and cooling blood. It has been reported in the literature that snakeberry has significant anti-tumor activity, but all the reports are the crude extracts of water or alcohol extraction of snakeberry...

Claims

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Application Information

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IPC IPC(8): A61K36/73A61K9/08A61K9/10A61K9/16A61K9/20A61P35/00A61P37/04
Inventor 刘新民常琪唐劲天王立为彭博胡琴王予祺
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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