Inflammation-induced protein component, and preparation method and product thereof

An inflammation-inducing and proteomic technology, applied in the field of biomedicine, can solve the problems of inappropriate control of dosage, large side effects, strong pro-inflammatory effect, etc., and achieve the effect of being suitable for large-scale production, mild pro-inflammatory effect, and clear pro-inflammatory effect.

Active Publication Date: 2015-07-01
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above deficiencies or improvement needs of the prior art, the present invention provides an inflammation-inducing protein component, its preparation method and product, the purpose of which is to provide a pro-inflammatory protein with stable and controllable pro-inflammatory effect and small side effects, thus Solve the technical problems that the current pro-inflammatory substances of lipopolysaccharide have strong pro-inflammatory effect, the dosage is not suitable for accurate control, and the side effects are large

Method used

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  • Inflammation-induced protein component, and preparation method and product thereof
  • Inflammation-induced protein component, and preparation method and product thereof
  • Inflammation-induced protein component, and preparation method and product thereof

Examples

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preparation example Construction

[0027] The inflammation-inducing protein component and its preparation method comprise the following steps:

[0028] (1) Peel the fresh lychee, remove the core and crush it to obtain a homogenate, and keep the supernatant after solid-liquid separation.

[0029] (2) Concentrate the supernatant obtained in step (1), and dialyze for 48 to 96 hours to obtain a crude extract with a cut-off between 8KD and 14KD; preferably, the dialysis time is 72 hours and the cut-off is 8KDa.

[0030] (3) Freeze-dry the crude extract obtained in step (2), extract the protein by phenol extraction, and dry it with nitrogen gas after precipitation with acetone to obtain the inflammation-inducing protein component.

[0031] The specific steps of protein extraction by phenol extraction are:

[0032] (3-1) Weighing the freeze-dried powder of the crude extract obtained in step (2) and adding it to the pre-cooled phenol extraction buffer to prepare a mixed solution, the amount added is between 5 mg / mL an...

Embodiment 1

[0040] An inflammation-inducing protein component is litchi protein extract, named LWP, analyzed by shotgun mass spectrometry, the peptide analysis results are shown in Table 1, and the protein analysis results obtained by searching the database (uniprot_malvids_14968_20140504) are shown in Table 2 The coverage of the detected peptides in each protein is described in detail in Table 2.

[0041] Table 1 Peptide list of protein components shotgun mass spectrometry analysis results

[0042]

[0043]

[0044]

[0045]

[0046]

[0047] Table 2 protein components shotgun mass spectrometry analysis results protein list

[0048]

[0049]

[0050] The inflammation-inducing protein component and its preparation method are as follows:

[0051] (1) Peel and remove the core of fresh lychees, put them into a juice extractor in batches to obtain a homogenate, filter with 4 layers of gauze, then centrifuge the filtrate at low temperature, and take the supernatant.

[0...

Embodiment 2

[0061] An inflammation-inducing protein component, which is lychee protein extract, named LWP, analyzed by shotgun mass spectrometry, protein analysis results, the detected proteins with a peptide coverage of more than 10% include: ADP-ribosylation factor 1, Glyceraldehyde -3-phosphate dehydrogenase GAPC1, Actin-7, 14-3-3-like protein GF14lambda, Ubiquitin-40S ribosomal protein S27a-1, 14-3-3-like protein GF14omega, 14-3-3-like protein GF14upsilon, Calmodulin-1, Eukaryotic initiation factor 4A-1.

[0062] The inflammation-inducing protein component and its preparation method are as follows:

[0063] (1) Fresh lychees are peeled and cored, put into a juice extractor in batches to obtain a homogenate, filtered with 4 layers of gauze, and then the filtrate is centrifuged at low temperature, and the supernatant is taken.

[0064] (2) The supernatant obtained in step (1) was freeze-dried and concentrated for two days, and dialyzed for 48 hours to obtain a crude extract with a cut-...

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Abstract

The invention discloses an inflammation-induced protein component, and a preparation method and a product thereof. The inflammation protein component is a litchi protein extract; the preparation method comprises the following steps: (1) removing peel of fresh litchi, removing core, squeezing to prepare homogenate, carrying out solid-liquid separation and reserving supernatant; (2) concentrating the supernatant prepared from the step (1), and dialyzing for 48-96 hours to obtain a crude extract, wherein the intercepted amount is between 8KD and 14KD; and (3) carrying out freeze drying on the crude extract prepared from the step (2), extracting protein employing a phenol extraction method, washing with acetone and blowing with nitrogen, so as to obtain the inflammation-induced protein component. The inflammation-induced protein component is used for preparing a proinflammatory preparation; the proinflammatory effect is specific, mild, easy to control, natural in component, free of a side effect and suitable for mass production.

Description

technical field [0001] The invention belongs to the field of biomedicine, and more specifically relates to an inflammation-inducing protein component, a preparation method and a product thereof. Background technique [0002] Pro-inflammatory substances are a class that can mediate a variety of immune responses. Recent studies have found that pro-inflammation can mediate tumor cell necrosis and is a promising anti-tumor substance. [0003] At present, lipopolysaccharide LPS, a commonly used pro-inflammatory substance in the market, has a strong pro-inflammatory effect, and its dosage is difficult to accurately control, with strong side effects. Therefore, continue to develop novel pro-inflammatory substances, such as inflammation-inducing proteins. Contents of the invention [0004] In view of the above deficiencies or improvement needs of the prior art, the present invention provides an inflammation-inducing protein component, its preparation method and product, the purp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/44A61K38/00A61K36/77A61P35/00C07K1/14C07K1/34
Inventor 马兆成王小丽邓秀新
Owner HUAZHONG AGRI UNIV
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