Method for extracting proteins from rubber trees and sesuvium portulacastrum plants by phenol extraction method

A plant protein and rubber tree technology, applied in chemical instruments and methods, peptide preparation methods, organic chemistry, etc., can solve problems that affect the separation of proteins by two-dimensional electrophoresis and interfere with mass spectrometry identification.

Pending Publication Date: 2021-02-05
HAINAN NORMAL UNIV +1
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Problems solved by technology

Plant tissues, especially rubber tree tissues, usually contain a large amount of recalcitrant substances, such as polysaccharides, quinones, and polyphenols. If the concentration of

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  • Method for extracting proteins from rubber trees and sesuvium portulacastrum plants by phenol extraction method
  • Method for extracting proteins from rubber trees and sesuvium portulacastrum plants by phenol extraction method
  • Method for extracting proteins from rubber trees and sesuvium portulacastrum plants by phenol extraction method

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Embodiment Construction

[0019] The present invention will be further described below based on the examples, and the modes of the present invention include but are not limited to the following examples.

[0020] like figure 1 As shown, the present invention comprises the following steps:

[0021] A After collecting plant tissue, grind the tissue sample in a liquid nitrogen quick-freezing environment to obtain plant tissue powder;

[0022] B. Add BPP protein extraction buffer to the plant tissue powder and perform mixed centrifugation to obtain the mixed solution and take the supernatant;

[0023] C. Take the supernatant and add a precipitant. After adding the supernatant and the precipitant according to the volume of 1:5, place the supernatant at -20° C. overnight to precipitate to obtain a vegetable protein precipitate.

[0024] D Wash the vegetable protein pellet to extract the protein.

[0025] In the present example, the fresh leaves (Hevea leaves, HL) of the rubber tree (Reyan 73-3-97), the fr...

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Abstract

The invention discloses a method for extracting proteins from rubber trees and sesuvium portulacastrum plants by a phenol extraction method. The method for extracting proteins from rubber trees and sesuvium portulacastrum plants comprises the following steps: collecting plant tissues, grinding tissue samples in a liquid nitrogen environment to obtain plant tissue powder; adding BPP extraction buffer solution to the plant tissue powder, carrying out mixed centrifugal treatment to obtain mixed solution, taking the supernatant of the mixed solution, adding a precipitant to the supernatant in 1:5of volume ratio of the supernatant to the precipitant, carrying out precipitation at -20 DEG C overnight to obtain plant protein precipitates, and washing the plant protein precipitates to extract proteins. According to the method of the present invention, proteins can be extracted from plants with polysaccharides and polyphenols or halophyte plants by combining different precipitants, and proteins in low-concentration solution can be extracted. The method of the present invention is suitable for extracting proteins from plants with polysaccharides, polyphenols and high salt content, and can also be used for obtaining proteins in high yield from recalcitrant plants.

Description

technical field [0001] The invention relates to the field of biological protein extraction, in particular to a method for extracting proteins from rubber trees and hippocampus plants by a phenol extraction method. Background technique [0002] In proteomics research, however, because various plant or tissue cells contain specific substances that can reduce the yield and quality of extracted proteins, it is impossible to extract proteins with a fixed yield, and specific extraction methods need to be adopted for specific materials. , thereby improving the quality of the extracted plant protein, which is also a key factor in determining the subsequent comparative analysis of proteomics. [0003] The Brazilian rubber tree (Hevea brasiliensis) is the only commercial crop currently cultivated on a large scale to produce natural rubber. In addition to polysaccharides, polyphenols and pigments, the leaves of rubber trees also contain substances such as cyanide and latex, and these ...

Claims

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Application Information

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IPC IPC(8): C07K1/30C07K1/14
CPCC07K1/30C07K1/145
Inventor 谢全亮王旭初
Owner HAINAN NORMAL UNIV
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