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Hot-dipping extraction method of crude plant polysaccharides

A crude polysaccharide and plant-based technology, which is applied in the field of hot-dip extraction of plant-derived crude polysaccharides, can solve the problems of lower activation energy, high equipment and instrument requirements, and increase the chance of collision, and achieve less interfering substances, a wide range of substances, and easy-to-obtain materials Effect

Inactive Publication Date: 2016-08-24
张政
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Problems solved by technology

However, this method also has some defects. Its extraction time should not be too long, and the power should not be too high, otherwise it will cause too much water evaporation, the dissolution of polysaccharides will be hindered, and the yield of polysaccharides will decrease; at the same time, this method also reduces some reactions. The activation energy of the polysaccharide can form new forces between polysaccharide molecules and between polysaccharide molecules and other molecules, increase the chance of collision between molecules, and prevent the dissolution of polysaccharide molecules; mass promotion

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment

[0016] A method for extracting crude polysaccharides from plants by hot soaking, the method steps are as follows:

[0017] (1) Accurately weigh the longan sample, add ultrapure water of the same quality according to the ratio of material to liquid of 1:1, beat in a homogenizer, transfer it to a 500mL beaker, place it in a constant temperature water bath at 80 degrees Celsius, and extract for 3 hours ;

[0018] (2) Put the leached slurry into a centrifuge for centrifugation, centrifuge at a speed of 8000r / min for 10min, and pour out the supernatant;

[0019] (3) According to the volume of the supernatant, according to the volume ratio of 1:4, add four times its volume of absolute ethanol to the supernatant to precipitate the polysaccharide;

[0020] (4) The mixture was refrigerated overnight at 4 degrees Celsius, and then centrifuged at 8000 r / min for 10 minutes to obtain a pale yellow precipitate;

[0021] (5) Repeatedly washing the precipitate with a small amount of dehydra...

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Abstract

The invention discloses a hot-dipping extraction method of crude plant polysaccharides. With a water system method, a plant sample for polysaccharide extraction is treated by five major steps including breaking and pulping, bath heating, centrifugation, washing and drying to finish crude extraction of plant polysaccharides; the extracted substances have a wide range; the method is suitable for extracting various plant polysaccharides and is easy to operate; the materials are easily available, the required conditions are simple, and the method is applicable to the extraction of free-state polysaccharides; and moreover, with few interfering substances, the method is safe to use in production, perfectly meets the practical requirements of domestic related industries and is suitable for large-scale promotion.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a method for extracting crude polysaccharides from plants by hot soaking. Background technique [0002] Plant polysaccharides, also known as plant polysaccharides, are polysaccharides with a degree of polymerization of more than 10 produced by plant cell metabolism. It is a compound composed of many identical or different monosaccharides with α- or β-glycosidic bonds. It is commonly found in plants in nature, including starch, cellulose, polysaccharides, pectin, etc. Due to the wide range of sources of plant polysaccharides, the molecular composition and molecular weight of different plant polysaccharides are different. Some plant polysaccharides such as starch, cellulose, and pectin have already become an important part of people's daily life. Plant polysaccharides are widely distributed in nature and are also very important. They participate in the immune regulation of org...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 张政
Owner 张政
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