Extraction method of 13KDa rice alcohol soluble protein
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A gliadin and extraction method technology, applied in the direction of plant protein processing, etc., can solve the problems of low extraction rate, low purity, and inability to large-scale industrial production, and achieve the effect of simple method and high purity
Inactive Publication Date: 2016-02-03
HARBIN INST OF TECH
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[0005] The present invention aims to solve the problems that the existing prolamin extraction method has low extraction rate, low purity and cannot be produced in a large-scale industrialized manner, and provides a method for extracting 13KDa rice prolamin
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specific Embodiment approach 1
[0018] Specific embodiment one: the extraction method of the present embodiment 13KDa rice prolamin, carries out according to the following steps:
[0019] 1. Grinding the rice into rice flour with a grinder;
[0020] 2. Degrease the rice flour with n-hexane for 3 to 5 times to obtain defatted rice flour;
[0021] 3. Extract the degreased rice flour with eluent for 3 to 5 times, and then wash the precipitate with pure water for 3 to 5 times;
[0022] Four, the precipitate after step 3 cleaning is leached with the n-propanol solution of 60% (v / v), and the supernatant is taken;
[0023] 5. The supernatant is precipitated with a trichloroacetic acid solution with a concentration of 10% to 12% (w / v);
[0024] 6. Vacuum freeze-dry the precipitate in step 5 to obtain 13KDa rice prolamin.
specific Embodiment approach 2
[0025] Embodiment 2: This embodiment is different from Embodiment 1 in that: the particle size of the rice flour in step 1 is 70-90 mesh. Others are the same as in the first embodiment.
specific Embodiment approach 3
[0026] Specific embodiment three: what this embodiment is different from specific embodiment one is: in step 2, the concrete method that rice flour is degreased with n-hexane is:
[0027] A. Mix rice flour and n-hexane according to the ratio of 1g:4-6mL, stir at room temperature for 1 hour, let stand for 1 hour, and then collect the precipitate after centrifuging at 4000rpm for 20min;
[0028] B. Repeat step A 2 to 4 times, and dry the collected precipitate at 30° C. to obtain defatted rice flour. Others are the same as in the first embodiment.
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Abstract
The present invention relates to an extraction method of 13KDa rice alcohol soluble protein to solve the problems that the existing extraction method of alcohol soluble protein is low in extraction rate, the purity of the extracted alcohol soluble protein is relatively low, and the existing extraction method cannot be applied to the mass industrial production. The extraction method includes: 1, grinding rice into flour with a grinder; 2, de-fatting the rice flour with n-hexane to obtain defatted rice flour; 3, leaching the defatted rice flour with elution liquid, and then washing the precipitates with pure water; 4, leaching the washed precipitates obtained in step 3 with n-propyl alcohol solution with a concentration of 60%, and collecting supernatant; 5, treating the supernatant with trichloroacetic acid solution to precipitate protein; and 6, performing vacuum freeze drying on the precipitates obtained in step 5, thereby obtaining the 13KDa rice alcohol soluble protein. The purity of the extracted rice alcohol soluble protein can reach 90%-94%, and the extraction method is suitable for large-scale industrialized extraction of the rice alcohol soluble protein.
Description
technical field [0001] The invention relates to a method for extracting rice prolamin. Background technique [0002] Rice protein is hypoallergenic and easily digested and absorbed by the body. Moreover, rice protein has the functions of lowering blood fat, lowering cholesterol, anti-oxidation, and preventing obesity. In particular, the latest research shows that rice protein has the effect of preventing diabetes. the attention of researchers around the world. [0003] Glamin is the main storage protein of rice protein, and its molecular weight is mainly 10, 13, 16KDa. Among them, 13KDa protein is the main protein body of rice prolamin, which has important physiological regulation function beneficial to health. Studies have shown that rice prolamin has a strong effect of adsorbing bile acids and can effectively prevent diseases such as hyperlipidemia. Moreover, due to the low digestibility of rice prolamin, it is the first choice for diabetics. But up to now, because the ...
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