46 results about "Protein body" patented technology

The invention discloses a Fanzor system-based gene editing tool suitable for plants. The Fanzor system-based gene editing tool comprises any one of the following units: (a) pH-SpuFz1-STU: a Ubi promoter, a SpuFz1 protein coding sequence, a connection sequence, a nuclear localization signal, PolyA, omega RNA (Ribonucleic Acid) of SpuFz1, a spacer region Spacer and an rbcS-E9 terminator; (b) a pH-GtFz1-STU: Ubi promoter, a GtFz1 protein coding sequence, a connection sequence, a nuclear localization signal, PolyA, omega RNA (Ribonucleic Acid) of GtFz1, a spacer region Spacer and an rbcS-E9 terminator; (c) a pH-NlovFz2-STU: a Ubi promoter, an NlovFz2 protein coding sequence, a connection sequence, a nuclear localization signal, PolyA, omega RNA (Ribonucleic Acid) of NlovFz2, a spacer region Spacer and an rbcS-E9 terminator; and (d) a pH-MmeFz2-STU: Ubi promoter, an MmeFz2 protein coding sequence, a connection sequence, a nuclear localization signal, PolyA, omega RNA (Ribonucleic Acid) of MmeFz2, a spacer region Spacer and an rbcS-E9 terminator. Effect protein of the Fanzor system is small in size, the protein structure is simple, and carrier construction and intercellular delivery are facilitated. And the Fanzor generates a DSB notch with a sticky tail end, so that deletion and insertion of a large fragment are facilitated. The vector is simple and efficient to assemble, and the existing plant gene editing tool box is effectively expanded and supplemented.

The invention relates to the technical field of aquaculture, in particular to nile tilapia ferritin, a preparation method and application thereof and a bacteriostatic agent. The amino acid sequence of the nile tilapia ferritin is as shown in SEQ ID No.1, and the nile tilapia ferritin inhibits streptococcus agalactiae and / or aeromonas hydrophila. The invention provides Nile tilapia ferritin, and in-vitro experiments prove that the recombinant protein can effectively inhibit proliferation of pathogenic bacteria. A further in-vivo experiment research result shows that the ferritin heavy chain (Nile tilapia ferritin) has important functions of inhibiting pathogenic bacterium proliferation and improving the survival rate of an organism, and plays an important role in immune protection of the organism.

The application belongs to the technical field of food processing, and particularly relates to an oat protein-pectin-proanthocyanidin compound and a preparation method thereof. The oat protein is mixed with water, and an alkali liquor is added to adjust the pH to obtain a protein material solution for medium grinding. Then, pectin is added to the ground protein material solution, and proanthocyanidin powder is added after the pH is adjusted, and secondary medium grinding is performed to obtain an oat protein-pectin-proanthocyanidin solution. Then, hydrochloric acid is slowly added to adjust the pH to 6.8-7.0. Finally, the protein solution is centrifuged, and the supernatant is spray dried to obtain the oat protein-pectin-proanthocyanidin compound. The oat protein-pectin-proanthocyanidin compound prepared by the method is a brand-new protein system, and solves the problems of poor dispersibility, nutritional defects and poor emulsifying property of oat protein, and has a wide application prospect.

The invention discloses a method for constructing an Asiatic corn borer GBP gene homozygous mutant, which comprises the following steps: amplifying and cloning an Asiatic corn borer GBP gene sequence by using RACE technology, and the nucleotide sequence of the Asiatic corn borer GBP gene is as shown in SEQ ID No.1; two sgRNA target sequences are designed on a second exon of a GBP gene sequence of the ostrinia furnacalis, target sgRNA is synthesized through in-vitro transcription, the target sgRNA and Cas9 protein are fused in vitro to form an RNP mixture, and the RNP mixture is injected into ovum of the ostrinia furnacalis; the method comprises the following steps: carrying out PCR (Polymerase Chain Reaction) amplification on a target spot by using a target spot detection primer, sequencing to confirm a mutant individual, hybridizing and selfing to obtain a GBP homozygous mutant population. The Asiatic corn borer GBP gene homozygous mutant is constructed, so that the reproduction and immune functions of the mutant are remarkably reduced, and reference and research basis are provided for later development and formation of efficient pest biopesticide.

The application discloses a microbial electrochemical system device and method for realizing wastewater treatment, CO2 fixation and in-situ synthesis of microbial protein synchronously, which comprises an anode chamber, a cathode chamber, a protein production chamber, a power supply, a mixed gas tank and a peristaltic pump; the anode chamber, the cathode chamber and the protein production chamber are sequentially arranged from left to right; a cation exchange membrane is arranged between the anode chamber and the cathode chamber; a cation exchange membrane is also arranged between the cathode chamber and the protein production chamber; an anode is arranged in the anode chamber and connected with the positive pole of the power supply through a wire, and a resistor is arranged on the wire connecting the anode and the positive pole of the power supply. The microbial electrochemical system and the protein production system are coupled to form an ammonia-nitrogen recovery-in-situ protein synthesis microbial electrochemical system, the system can convert active nitrogen into high-value protein products in-situ, the system can synchronously treat wastewater, recover ammonia-nitrogen and fix carbon dioxide, and has excellent environmental benefits.

The present invention belongs to the field of biopharmaceuticals, and particularly relates to a multi-domain active protein for treating metabolic diseases. The multi-domain active protein has a structural formula as shown in Formula I: A-L a -F-L b -B. Compared with the prior art, the multi-domain active protein of the present invention has a long half-life and supports a once-weekly dosing frequency; the GLP-1R agonist activity of the multi-domain active protein is increased by more than 200 times at most; the multi-domain active protein has good stability in vivo and in vitro and low immunogenicity; since no unnatural amino acids need to be introduced and no chemical synthesis and cross-linking steps are involved, it can be prepared by a recombinant method, greatly simplifying the preparation process.

The invention relates to the technical field of dairy product processing, in particular to a double-fat double-protein synergistic dairy product and a preparation method thereof. According to the preparation method, a ''whey protein (WP)-casein phosphopeptide (CPP)'' double-protein system and a ''milk fat (MF)-medium chain triglyceride (MCT)'' double-fat system are constructed, and a four-step synergistic process of ''pretreatment, composite emulsification, gradient assembly and low-temperature curing'' is adopted, so that stable combination of a chemical interface of protein and fat is realized, high-temperature stability breakthrough is realized, and the preparation method has the advantages of high-temperature stability, high-temperature stability and high-temperature stability. Meanwhile, high-pressure processing tolerance is improved, fat globule flocculation caused by high pressure is avoided, and long-term storage stability, texture and taste are guaranteed.

The invention relates to the technical field of nerve cell marking and regulation and control, in particular to a nerve cell living body selective protein modification probe capable of penetrating a blood brain barrier and application of the nerve cell living body selective protein modification probe. The protein body part specifically targets a living glutamic acid energetic neuron cell membrane, the protein body part and / or a modified part thereof can regulate and control the function of the glutamic acid energetic neuron, and a coding sequence of the protein body part comprises a sequence as shown in SEQ ID NO: 1 or a sequence having at least 85% identity with the SEQ ID NO: 1. The protein modified probe provided by the invention provides a new feasible thought for developing in-vivo nerve cell selective markers and regulation tools which are non-gene transfection of blood brain barrier penetration, are high in biological safety, are more stable and are easy to obtain and have nerve cell specific type selectivity.

The invention relates to the technical field of dairy product processing, and discloses a method for improving the stability of ultrahigh-temperature sterilized goat milk. Gradient decalcification regulation and control are performed on a sheep milk casein system by adopting an ultra-high-speed centrifugal dilution method, and a sheep milk casein calcium stabilizing environment adaptive to ultra-high-temperature sterilization is constructed in combination with calcium ion back-adding verification. Experiments show that the particle size of the decalcified sheep milk casein micelle is reduced, the absolute value of Zeta potential is remarkably increased, and the thermal coagulation time is greatly prolonged. When the decalcification rate of the goat milk casein reaches 76%, the thermal coagulation time of the goat milk casein is prolonged to 180 minutes or more when the goat milk casein is heated at 95 DEG C, no precipitate is generated, and the thermal stability of the goat milk casein is superior to that of untreated goat milk and bovine milk casein. The technical problems that the goat milk is easy to aggregate and precipitate and poor in thermal stability under the ultrahigh-temperature sterilization condition are solved, the process is simple and convenient, no exogenous additive exists, the stability of the product can be remarkably improved, the shelf life of the product can be remarkably prolonged, and reliable theoretical basis and technical support are provided for development of high-quality normal-temperature liquid goat milk products.

The application discloses a fusion protein, an in-vivo directed evolution system and application thereof, and the fusion protein comprises a Retron reverse transcriptase and an adenosine deaminase, and the adenosine deaminase is connected to the N terminal or C terminal of the Retron reverse transcriptase. The application firstly fuses the Retron reverse transcriptase with the adenosine deaminase TadA-8e, constructs a bifunctional fusion protein system with reverse transcription activity and adenosine deamination activity, realizes the functional coupling of Retron-mediated msDNA synthesis and adenosine deamination mutation introduction. The application significantly improves the mutation frequency and mutation diversity of a target gene, accelerates the generation, accumulation and fixation of beneficial mutations, and thus improves the in-vivo directed evolution efficiency.

The application discloses an in-vitro detection method and kit for O-GlcNAc glycosylation modification of alpha-synuclein, and relates to the field of biochemical detection. Y289L The method comprises the following steps: extracting protein in a sample to be detected, adding UDP-GalNAz substrate beta4GalT Y289L The mutant enzyme and the reaction are carried out to obtain a protein system; DBCO-mPEG is added to the protein system, and the reaction product is subjected to electrophoretic separation; and according to an electrophoretogram, the proportion of the protein subjected to glycosylation modification and the molecular weight distribution thereof are calculated. The application marks specific molecular weight "tags" for O-GlcNAc glycosylation proteins, and the glycosylation-modified proteins are recognized by alpha-synuclein specific antibodies to show specific molecular weight sizes; and the alpha-synuclein polymerization states, such as dimers and polymers, subjected to O-GlcNAc glycosylation modification can be determined and quantified according to protein standards.

A system and method for producing, on a commercial scale, plant or reconstituted meat based products, such as pet food products having a main protein body and chunks of a non-protein component, by pumping of a protein slurry (15) to a heating device, such as a vacuum supply unit pumping the protein slurry. The heating device includes a heated die passage (275) through which the slurry (15) is directed, with the slurry (15) being heated along both inner and outer surfaces while transitioning into a heated protein product (35) which flows out of a die head (130) with the chunk ingredients intact and dispersed about the main protein body.

The invention relates to a plant glutelin sorting related protein OsVPS34 as well as a coding gene and application thereof. The protein provided by the invention is a protein as shown in (a) or (b): (a) a protein consisting of an amino acid sequence as shown in SEQ ID NO.3 in a sequence table; and (b) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence of SEQ ID NO.3, is related to plant glutelin sorting and is derived from SEQ ID NO.3. The protein provided by the invention affects the sorting of glutelin in plant endosperm, and the glutelin cannot be correctly transported into a type II protein body, so that the glutelin content in mature seeds is reduced, and a glutelin precursor is increased. The coding gene of the protein is introduced into a plant with abnormal glutelin sorting, and a transgenic plant with normal glutelin sorting can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

The present application relates to the field of aquaculture technology, in particular to a tilapia nilotica ferritin, a preparation method and application thereof, and a bacteriostatic agent.The amino acid sequence of the tilapia nilotica ferritin is shown as SEQ ID No.1, and the tilapia nilotica ferritin can inhibit Streptococcus agalactiae and / or Aeromonas hydrophila.The present application provides the tilapia nilotica ferritin, and in-vitro experiments have proved that the recombinant protein can effectively inhibit the proliferation of pathogenic bacteria.Further in-vivo experimental results show that the ferritin heavy chain (tilapia nilotica ferritin) has the important functions of inhibiting the proliferation of pathogenic bacteria and improving the survival rate of the organism, and plays an important role in the immune protection of the organism.

1. Name of the product of this design: Packaging box (protein in vitro companion diagnostic reagent 2). 2. Purpose of this design product: This design product is used for packaging products. 3. The key design point of this design product lies in the combination of shape and pattern. 4. The picture or photo that best illustrates the design points: Stereoscopic drawing 1. 5. Other situations that require explanation Other explanations: The gray square coated area in the right view and stereoscopic view 1 is the QR code display area.

The present invention proposes a milk nano-vesicle loading material with high transdermal performance and its preparation method and application. The milk is adjusted to pH and centrifuged to obtain protein bodies and supernatant. The supernatant is ultrafiltered to obtain milk extracellular vesicles. The milk nano-vesicles are heated and irradiated to obtain milk nano-vesicles. The protein bodies are reacted with phytic acid and β-cyclodextrin to obtain β-cyclodextrin / phytic acid / milk nano-vesicle complexes. The protein bodies are reacted with alkylated beauty peptides to obtain peptide-loaded protein bodies. The protein bodies are embedded with β-cyclodextrin / phytic acid / milk nano-vesicle complexes, vitamin E, and vitamin C to obtain a milk nano-vesicle loading material with high transdermal performance. The milk nano-vesicle loading material with high transdermal performance has good stability, a high loading capacity for protein peptides, and a good transdermal absorption-promoting effect. It can effectively resist aging, improve skin elasticity, reduce fine lines, and promote skin repair, regeneration, and protection.

The present invention relates to a proteasome degradative protein complex comprising an E3 ubiquitin ligase component tethered to an alpha-synuclein specific polypeptide binding agent for the degradation of alpha-synuclein. The present invention also includes mechanisms of action, compositions, and related methods for the treatment of neurological disorders, including synucleinopathies.

The present invention provides a method for preparing protein-containing FNL (FNL), belonging to the field of biomedicine. The method comprises: dissolving soy lecithin and cholesterol in a solvent and rotary evaporating to obtain a thin film; dissolving sodium alginate, allantoin, and fibronectin in a buffer to obtain a protein solution; mixing the protein solution with the obtained thin film and rotating to obtain a rotating solution; and ultrasonicating the obtained rotating solution and extruding it through a microporous filter membrane to obtain protein-containing FNL. Sodium alginate and allantoin enhance the transdermal absorption rate of fibronectin, reaching over 70% after 24 hours, an increase of over 22% compared to the rate without sodium alginate and allantoin.

The invention discloses two odontotermes formosanus OfCTLs genes and application of dsRNA of the odontotermes formosanus OfCTLs genes. Wherein the two odontotermes formosanus OfCTLs genes are an OfCTL-S3 gene and an OfCTL-S15 gene respectively, and the corresponding nucleotide sequences of the odontotermes formosanus OfCTL-S3 gene and the OfCTL-S15 gene are SEQ ID NO: 1 and SEQ ID NO: 2 respectively. Recombinant OfCTL-S3 and OfCTL-S15 proteins are obtained through a prokaryotic expression system, and an in-vitro antibacterial test proves that the OfCTL-S3 and OfCTL-S15 proteins have antibacterial activity and agglutination effects on beauveria bassiana, gram-positive bacterium bacillus thuringiensis and gram-negative bacterium serratia marcescens in the presence of calcium ions. According to the invention, an odontotermes formosanus OfCTLs RNA interference system carried out by using a feeding method is established, and an obvious gene silencing effect is obtained by feeding odontotermes formosanus. The dsRNA of the odontotermes formosanus OfCTLs is combined with the biocontrol bacteria, so that the control effect of the biocontrol bacteria can be remarkably improved.

The invention discloses a protein-DNA interaction detection method aiming at whole genome open chromatin and independent of an antibody. The method comprises the steps of cell nucleus preparation, transposase reaction, ampDAP library construction, protein incubation and DNA library construction. The cell nucleus preparation comprises cell activity detection and cell nucleus extraction; the transposase reaction comprises transposon and DNA (Deoxyribose Nucleic Acid) recovery; the construction of the ampDAP library comprises the steps of PCR amplification and product purification; the protein incubation comprises protein in-vitro expression and protein-library incubation; dNA library building comprises PCR (polymerase chain reaction) amplification and product purification. According to the present invention, the protein-DNA interaction detection is achieved, the experiment target can be achieved by only requiring the in-vitro expression of the interested protein during the process, and the one library can simultaneously meet the detection of a plurality of proteins, such that the new idea and the new method are provided for the protein-DNA interaction detection.

1. Name of the product of this design: Packaging box (protein in vitro companion diagnostic reagent 1). 2. Purpose of this design product: This design product is used for packaging products. 3. The key design point of this design product lies in the combination of shape and pattern. 4. The picture or photo that best illustrates the design points: Stereoscopic drawing 1. 5. Other situations that require explanation: The gray square coated area in the top view, stereoscopic view 1, and stereoscopic view 2 is the QR code display area.

The invention discloses a compound plant double-type beverage containing medicinal and edible raw materials such as gastrodia elata and a preparation method thereof. The compound plant double-type beverage comprises two types of products, namely a plant protein type product and a protein-free green juice type product. Through a vegetable protein embedding technology and a green juice fiber embedding technology, the problems of peculiar smell of gastrodia elata, protein system separation chromatography oil, rough taste of green juice, easy browning and the like are solved, and the drinking smoothness and flavor coordination are improved. The product is free of pigments, preservatives and essences, meets modern healthy consumption requirements, and is excellent in normal-temperature storage stability. The technology is high in standardization degree and suitable for industrial production, the double-product structure can meet different taste preferences and requirements of consumer groups, and good market application prospects are achieved.

The invention belongs to the technical field of biological genetic engineering, and particularly relates to a corn kernel development regulation gene ZmPPR299, an encoding protein thereof, an SNP (Single Nucleotide Polymorphism) site, a molecular marker and application of the molecular marker, the gene ZmPPR299 is located in a corn chromosome 5, and functional verification shows that the gene encodes a PPR protein located in chloroplast, and the molecular marker can be used for regulating the development of corn kernels. The chloroplast gene is an essential gene for regulating corn kernel development and chloroplast functions; the mutation of the gene can cause the lagging of embryo and endosperm development, the reduction of basal transfer layer cell proliferation, and the insufficient filling of starch grains and protein bodies, and finally, the results show that the grains shrink, the hundred-grain weight is obviously reduced, and the leaves of seedlings lose green. The invention also develops a specific CAPS molecular marker and a detection primer thereof based on a single base mutation site of the gene, and the genotype of the mutant can be rapidly and accurately identified. The gene resource and the molecular marker provided by the invention have important application values in analysis of a corn kernel development regulation network, development of molecular assisted breeding and creation of high-yield and high-quality new corn germplasm.

The invention provides a cow milk nano-vesicle load with high transdermal performance and a preparation method and application thereof.The preparation method comprises the steps that cow milk is adjusted in pH, left to stand and centrifuged to obtain a protein body and supernate, the supernate is subjected to ultrafiltration to obtain cow milk extracellular vesicles, the cow milk extracellular vesicles are subjected to heating and irradiation treatment to obtain cow milk nano-vesicles, and then the cow milk nano-vesicles are loaded into the cow milk nano-vesicles. Reacting with phytic acid and beta-cyclodextrin to prepare a beta-cyclodextrin / phytic acid / cow milk nano-vesicle compound; the protein body reacts with alkylated cosmetic peptide to prepare a peptide-loaded protein body, and the peptide-loaded protein body is embedded with a beta-cyclodextrin / phytic acid / cow milk nano-vesicle compound, vitamin E and vitamin C to prepare the cow milk nano-vesicle loading material with high transdermal performance. The cow milk nano-vesicle loading material with high transdermal performance has relatively good stability, is high in loading capacity of protein peptide, has a very good transdermal absorption promoting effect, and can well achieve the effects of resisting aging, improving skin elasticity, reducing fine wrinkles and promoting skin repair, regeneration and protection.

A method for the directed evolution of proteins. The method comprises obtaining a PCR product with a gene mutation of a target protein by means of PCR amplification; placing the PCR product in an in-vitro protein expression system for gene expression to obtain a target protein with a mutation; and performing a characterization detection on the target protein with the mutation, wherein the target protein is a target enzyme, and the characterization detection comprises detecting the activity of the target enzyme and / or the corresponding enantiomeric excess. The method not only has high stability, but also shortens the time from a generally required 2-3 weeks to about 7 hours, which greatly accelerates the evolution speed.

The invention belongs to the technical field of microorganisms, and particularly relates to application of lactobacillus paracasei as a double-protein leavening agent and fermentation liquor. The lactobacillus paracasei is the lactobacillus paracasei JM053; and the double proteins are soybean protein isolate and whey protein isolate. Researches find that the lactobacillus paracasei JM053 not only has good hydrolysis and enzymolysis effects on double proteins, but also can significantly reduce the antigenicity / sensitization of a double-protein fermentation product, and can unexpectedly improve the content of water-soluble proteins in the fermentation product and the antioxidant activity of the fermentation product compared with a fermentation single protein. Therefore, the strain has a good industrial application prospect as a double-protein system leavening agent, and the double-protein fermentation liquor also has a good industrial application prospect as a low-antigenicity and high-oxidation-resistance substance.

This invention discloses a compound probiotic feed for livestock and poultry that optimizes protein and energy supply, and its preparation method. In this invention, by flexibly adjusting the ratio of protein raw materials and energy components, targeted nutritional supply is provided for growing-finishing pigs, commercial laying hens, and broiler poultry. Fermented soybean meal and fermented alfalfa meal, after premixing and fermentation, not only retain the natural nutrients in the raw materials but also degrade anti-nutritional factors through microbial fermentation. Combined with high-quality animal protein from imported fishmeal, a protein system more easily digested and absorbed by livestock and poultry is constructed. The addition of the compound probiotic preparation can form a dominant bacterial community environment in the livestock and poultry intestines, promoting intestinal peristalsis and digestive enzyme secretion, further improving the conversion rate of nutrients in the feed, reducing nutrient loss in the intestines, helping livestock and poultry grow rapidly, improving overall breeding efficiency, effectively improving the intestinal health of livestock and poultry, and reducing the incidence of diseases during the breeding process.

Polynucleotides such as DNA are stored inside vesicles formed from self-assembling membranes. The vesicles may be protocells, liposomes, micelles, colloidosomes, proteinosomes, or coacervates. The vesicles may include surface functionalization to improve polynucleotide encapsulation and / or to bind polynucleotides having specific sequences. Encapsulation in vesicles provides protection for the polynucleotides. Additional protection is provided by addition of one or more stabilizers. The stabilizer may be nucleic-acid stabilizers that stabilize the polynucleotides or may be a protective structural layer around the vesicles such as a layer of silica. A process for stably storing polynucleotides in vesicles and a process for recovering stored polynucleotides from vesicles are both disclosed. The polynucleotides may be used for storage of digital information.

The invention discloses an MG53 protein bionic nano-drug and a preparation method thereof, nano mesoporous silica is used as a carrier of MG53 protein, the MG53 protein is loaded in silica mesopores through electrostatic interaction, and the surface of the mesoporous silica is coated with an erythrocyte membrane; the invention further discloses application of the MG53 protein bionic nano-drug in preparation of drugs for treating and / or preventing myocardial infarction and cardiac function recovery after myocardial infarction and application of the MG53 protein bionic nano-drug in preparation of drugs for treating and / or preventing heart injury. The protein bionic nano-drug provides a new method for intracellular delivery of high-molecular-weight protein, is expected to provide a new drug and an effective protection measure for myocardial infarction protection, and provides a new idea for in-vivo treatment of MG53 protein.

The invention discloses a method for improving lipid loss resistance of an emulsified fish product based on polyphenol modified compound protein, and belongs to the technical field of food processing. The method for improving the lipid loss resistance of the emulsified fish product specifically comprises the following steps: forming a compound protein system by using MP of fish meat and plant source protein, modifying the compound protein system by adopting polyphenol, adding fish oil into the polyphenol modified MP-SPI compound system, homogenizing and emulsifying, heating and inducing to gelatinize, and cooling, so as to obtain the emulsified fish product. The emulsified fish product obtained by the method shows enhanced interfacial strength and oxidation resistance, significantly inhibits lipid droplet coalescence and oxidation loss in the hot working process, reduces the lipid loss rate by 40% or more after heat treatment, has stable texture and high nutrition retention rate, does not need chemical additives, and is suitable for industrial production. The method is suitable for producing functional emulsified fish products rich in highly unsaturated fatty acids, and has a wide practical application prospect.