24 results about "Protein body" patented technology

The invention relates to the technical field of aquaculture, in particular to nile tilapia ferritin, a preparation method and application thereof and a bacteriostatic agent. The amino acid sequence of the nile tilapia ferritin is as shown in SEQ ID No.1, and the nile tilapia ferritin inhibits streptococcus agalactiae and / or aeromonas hydrophila. The invention provides Nile tilapia ferritin, and in-vitro experiments prove that the recombinant protein can effectively inhibit proliferation of pathogenic bacteria. A further in-vivo experiment research result shows that the ferritin heavy chain (Nile tilapia ferritin) has important functions of inhibiting pathogenic bacterium proliferation and improving the survival rate of an organism, and plays an important role in immune protection of the organism.

The application discloses a microbial electrochemical system device and method for realizing wastewater treatment, CO2 fixation and in-situ synthesis of microbial protein synchronously, which comprises an anode chamber, a cathode chamber, a protein production chamber, a power supply, a mixed gas tank and a peristaltic pump; the anode chamber, the cathode chamber and the protein production chamber are sequentially arranged from left to right; a cation exchange membrane is arranged between the anode chamber and the cathode chamber; a cation exchange membrane is also arranged between the cathode chamber and the protein production chamber; an anode is arranged in the anode chamber and connected with the positive pole of the power supply through a wire, and a resistor is arranged on the wire connecting the anode and the positive pole of the power supply. The microbial electrochemical system and the protein production system are coupled to form an ammonia-nitrogen recovery-in-situ protein synthesis microbial electrochemical system, the system can convert active nitrogen into high-value protein products in-situ, the system can synchronously treat wastewater, recover ammonia-nitrogen and fix carbon dioxide, and has excellent environmental benefits.

The invention relates to the technical field of dairy product processing, in particular to a double-fat double-protein synergistic dairy product and a preparation method thereof. According to the preparation method, a ''whey protein (WP)-casein phosphopeptide (CPP)'' double-protein system and a ''milk fat (MF)-medium chain triglyceride (MCT)'' double-fat system are constructed, and a four-step synergistic process of ''pretreatment, composite emulsification, gradient assembly and low-temperature curing'' is adopted, so that stable combination of a chemical interface of protein and fat is realized, high-temperature stability breakthrough is realized, and the preparation method has the advantages of high-temperature stability, high-temperature stability and high-temperature stability. Meanwhile, high-pressure processing tolerance is improved, fat globule flocculation caused by high pressure is avoided, and long-term storage stability, texture and taste are guaranteed.

The invention relates to the technical field of dairy product processing, and discloses a method for improving the stability of ultrahigh-temperature sterilized goat milk. Gradient decalcification regulation and control are performed on a sheep milk casein system by adopting an ultra-high-speed centrifugal dilution method, and a sheep milk casein calcium stabilizing environment adaptive to ultra-high-temperature sterilization is constructed in combination with calcium ion back-adding verification. Experiments show that the particle size of the decalcified sheep milk casein micelle is reduced, the absolute value of Zeta potential is remarkably increased, and the thermal coagulation time is greatly prolonged. When the decalcification rate of the goat milk casein reaches 76%, the thermal coagulation time of the goat milk casein is prolonged to 180 minutes or more when the goat milk casein is heated at 95 DEG C, no precipitate is generated, and the thermal stability of the goat milk casein is superior to that of untreated goat milk and bovine milk casein. The technical problems that the goat milk is easy to aggregate and precipitate and poor in thermal stability under the ultrahigh-temperature sterilization condition are solved, the process is simple and convenient, no exogenous additive exists, the stability of the product can be remarkably improved, the shelf life of the product can be remarkably prolonged, and reliable theoretical basis and technical support are provided for development of high-quality normal-temperature liquid goat milk products.

The application discloses a fusion protein, an in-vivo directed evolution system and application thereof, and the fusion protein comprises a Retron reverse transcriptase and an adenosine deaminase, and the adenosine deaminase is connected to the N terminal or C terminal of the Retron reverse transcriptase. The application firstly fuses the Retron reverse transcriptase with the adenosine deaminase TadA-8e, constructs a bifunctional fusion protein system with reverse transcription activity and adenosine deamination activity, realizes the functional coupling of Retron-mediated msDNA synthesis and adenosine deamination mutation introduction. The application significantly improves the mutation frequency and mutation diversity of a target gene, accelerates the generation, accumulation and fixation of beneficial mutations, and thus improves the in-vivo directed evolution efficiency.

The application discloses an in-vitro detection method and kit for O-GlcNAc glycosylation modification of alpha-synuclein, and relates to the field of biochemical detection. Y289L The method comprises the following steps: extracting protein in a sample to be detected, adding UDP-GalNAz substrate beta4GalT Y289L The mutant enzyme and the reaction are carried out to obtain a protein system; DBCO-mPEG is added to the protein system, and the reaction product is subjected to electrophoretic separation; and according to an electrophoretogram, the proportion of the protein subjected to glycosylation modification and the molecular weight distribution thereof are calculated. The application marks specific molecular weight "tags" for O-GlcNAc glycosylation proteins, and the glycosylation-modified proteins are recognized by alpha-synuclein specific antibodies to show specific molecular weight sizes; and the alpha-synuclein polymerization states, such as dimers and polymers, subjected to O-GlcNAc glycosylation modification can be determined and quantified according to protein standards.

A system and method for producing, on a commercial scale, plant or reconstituted meat based products, such as pet food products having a main protein body and chunks of a non-protein component, by pumping of a protein slurry (15) to a heating device, such as a vacuum supply unit pumping the protein slurry. The heating device includes a heated die passage (275) through which the slurry (15) is directed, with the slurry (15) being heated along both inner and outer surfaces while transitioning into a heated protein product (35) which flows out of a die head (130) with the chunk ingredients intact and dispersed about the main protein body.

The invention relates to a plant glutelin sorting related protein OsVPS34 as well as a coding gene and application thereof. The protein provided by the invention is a protein as shown in (a) or (b): (a) a protein consisting of an amino acid sequence as shown in SEQ ID NO.3 in a sequence table; and (b) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence of SEQ ID NO.3, is related to plant glutelin sorting and is derived from SEQ ID NO.3. The protein provided by the invention affects the sorting of glutelin in plant endosperm, and the glutelin cannot be correctly transported into a type II protein body, so that the glutelin content in mature seeds is reduced, and a glutelin precursor is increased. The coding gene of the protein is introduced into a plant with abnormal glutelin sorting, and a transgenic plant with normal glutelin sorting can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

The present application relates to the field of aquaculture technology, in particular to a tilapia nilotica ferritin, a preparation method and application thereof, and a bacteriostatic agent.The amino acid sequence of the tilapia nilotica ferritin is shown as SEQ ID No.1, and the tilapia nilotica ferritin can inhibit Streptococcus agalactiae and / or Aeromonas hydrophila.The present application provides the tilapia nilotica ferritin, and in-vitro experiments have proved that the recombinant protein can effectively inhibit the proliferation of pathogenic bacteria.Further in-vivo experimental results show that the ferritin heavy chain (tilapia nilotica ferritin) has the important functions of inhibiting the proliferation of pathogenic bacteria and improving the survival rate of the organism, and plays an important role in the immune protection of the organism.

The invention discloses two odontotermes formosanus OfCTLs genes and application of dsRNA of the odontotermes formosanus OfCTLs genes. Wherein the two odontotermes formosanus OfCTLs genes are an OfCTL-S3 gene and an OfCTL-S15 gene respectively, and the corresponding nucleotide sequences of the odontotermes formosanus OfCTL-S3 gene and the OfCTL-S15 gene are SEQ ID NO: 1 and SEQ ID NO: 2 respectively. Recombinant OfCTL-S3 and OfCTL-S15 proteins are obtained through a prokaryotic expression system, and an in-vitro antibacterial test proves that the OfCTL-S3 and OfCTL-S15 proteins have antibacterial activity and agglutination effects on beauveria bassiana, gram-positive bacterium bacillus thuringiensis and gram-negative bacterium serratia marcescens in the presence of calcium ions. According to the invention, an odontotermes formosanus OfCTLs RNA interference system carried out by using a feeding method is established, and an obvious gene silencing effect is obtained by feeding odontotermes formosanus. The dsRNA of the odontotermes formosanus OfCTLs is combined with the biocontrol bacteria, so that the control effect of the biocontrol bacteria can be remarkably improved.

The invention discloses a compound plant double-type beverage containing medicinal and edible raw materials such as gastrodia elata and a preparation method thereof. The compound plant double-type beverage comprises two types of products, namely a plant protein type product and a protein-free green juice type product. Through a vegetable protein embedding technology and a green juice fiber embedding technology, the problems of peculiar smell of gastrodia elata, protein system separation chromatography oil, rough taste of green juice, easy browning and the like are solved, and the drinking smoothness and flavor coordination are improved. The product is free of pigments, preservatives and essences, meets modern healthy consumption requirements, and is excellent in normal-temperature storage stability. The technology is high in standardization degree and suitable for industrial production, the double-product structure can meet different taste preferences and requirements of consumer groups, and good market application prospects are achieved.

The invention belongs to the technical field of biological genetic engineering, and particularly relates to a corn kernel development regulation gene ZmPPR299, an encoding protein thereof, an SNP (Single Nucleotide Polymorphism) site, a molecular marker and application of the molecular marker, the gene ZmPPR299 is located in a corn chromosome 5, and functional verification shows that the gene encodes a PPR protein located in chloroplast, and the molecular marker can be used for regulating the development of corn kernels. The chloroplast gene is an essential gene for regulating corn kernel development and chloroplast functions; the mutation of the gene can cause the lagging of embryo and endosperm development, the reduction of basal transfer layer cell proliferation, and the insufficient filling of starch grains and protein bodies, and finally, the results show that the grains shrink, the hundred-grain weight is obviously reduced, and the leaves of seedlings lose green. The invention also develops a specific CAPS molecular marker and a detection primer thereof based on a single base mutation site of the gene, and the genotype of the mutant can be rapidly and accurately identified. The gene resource and the molecular marker provided by the invention have important application values in analysis of a corn kernel development regulation network, development of molecular assisted breeding and creation of high-yield and high-quality new corn germplasm.

A method for the directed evolution of proteins. The method comprises obtaining a PCR product with a gene mutation of a target protein by means of PCR amplification; placing the PCR product in an in-vitro protein expression system for gene expression to obtain a target protein with a mutation; and performing a characterization detection on the target protein with the mutation, wherein the target protein is a target enzyme, and the characterization detection comprises detecting the activity of the target enzyme and / or the corresponding enantiomeric excess. The method not only has high stability, but also shortens the time from a generally required 2-3 weeks to about 7 hours, which greatly accelerates the evolution speed.

The invention belongs to the technical field of microorganisms, and particularly relates to application of lactobacillus paracasei as a double-protein leavening agent and fermentation liquor. The lactobacillus paracasei is the lactobacillus paracasei JM053; and the double proteins are soybean protein isolate and whey protein isolate. Researches find that the lactobacillus paracasei JM053 not only has good hydrolysis and enzymolysis effects on double proteins, but also can significantly reduce the antigenicity / sensitization of a double-protein fermentation product, and can unexpectedly improve the content of water-soluble proteins in the fermentation product and the antioxidant activity of the fermentation product compared with a fermentation single protein. Therefore, the strain has a good industrial application prospect as a double-protein system leavening agent, and the double-protein fermentation liquor also has a good industrial application prospect as a low-antigenicity and high-oxidation-resistance substance.

This invention discloses a compound probiotic feed for livestock and poultry that optimizes protein and energy supply, and its preparation method. In this invention, by flexibly adjusting the ratio of protein raw materials and energy components, targeted nutritional supply is provided for growing-finishing pigs, commercial laying hens, and broiler poultry. Fermented soybean meal and fermented alfalfa meal, after premixing and fermentation, not only retain the natural nutrients in the raw materials but also degrade anti-nutritional factors through microbial fermentation. Combined with high-quality animal protein from imported fishmeal, a protein system more easily digested and absorbed by livestock and poultry is constructed. The addition of the compound probiotic preparation can form a dominant bacterial community environment in the livestock and poultry intestines, promoting intestinal peristalsis and digestive enzyme secretion, further improving the conversion rate of nutrients in the feed, reducing nutrient loss in the intestines, helping livestock and poultry grow rapidly, improving overall breeding efficiency, effectively improving the intestinal health of livestock and poultry, and reducing the incidence of diseases during the breeding process.

Polynucleotides such as DNA are stored inside vesicles formed from self-assembling membranes. The vesicles may be protocells, liposomes, micelles, colloidosomes, proteinosomes, or coacervates. The vesicles may include surface functionalization to improve polynucleotide encapsulation and / or to bind polynucleotides having specific sequences. Encapsulation in vesicles provides protection for the polynucleotides. Additional protection is provided by addition of one or more stabilizers. The stabilizer may be nucleic-acid stabilizers that stabilize the polynucleotides or may be a protective structural layer around the vesicles such as a layer of silica. A process for stably storing polynucleotides in vesicles and a process for recovering stored polynucleotides from vesicles are both disclosed. The polynucleotides may be used for storage of digital information.

The invention discloses a method for improving lipid loss resistance of an emulsified fish product based on polyphenol modified compound protein, and belongs to the technical field of food processing. The method for improving the lipid loss resistance of the emulsified fish product specifically comprises the following steps: forming a compound protein system by using MP of fish meat and plant source protein, modifying the compound protein system by adopting polyphenol, adding fish oil into the polyphenol modified MP-SPI compound system, homogenizing and emulsifying, heating and inducing to gelatinize, and cooling, so as to obtain the emulsified fish product. The emulsified fish product obtained by the method shows enhanced interfacial strength and oxidation resistance, significantly inhibits lipid droplet coalescence and oxidation loss in the hot working process, reduces the lipid loss rate by 40% or more after heat treatment, has stable texture and high nutrition retention rate, does not need chemical additives, and is suitable for industrial production. The method is suitable for producing functional emulsified fish products rich in highly unsaturated fatty acids, and has a wide practical application prospect.

The application discloses a method for constructing a homozygous mutant of Ostrinia furnacalis GBP Gu, and cloning the Ostrinia furnacalis GBP Gene sequence by using RACE technology, and the nucleotide sequence of the Ostrinia furnacalis GBP Gene is shown as SEQ ID No. 1; the 2nd exon of the Ostrinia furnacalis GBP Gene sequence is designed with two sgRNA target sequences, target sgRNA is synthesized by in-vitro transcription, target sgRNA is fused with Cas9 protein in-vitro to form an RNP mixture, the RNP mixture is injected into Ostrinia furnacalis eggs; target points are PCR amplified by using target detection primers, mutant individuals are confirmed by sequencing, hybridization and self-crossing to obtain GBP A homozygous mutant population. The application constructs the Ostrinia furnacalis GBP GBP GBP GBP GBP GBP GBP GBP Homozygous mutant, which leads to a significant decrease in the reproduction and immune function of the mutant, and provides a reference and research basis for developing a high-efficiency biological pesticide of pests in the later stage.

Polynucleotides such as DNA are stored inside vesicles formed from self-assembling membranes. The vesicles may be protocells, liposomes, micelles, colloidosomes, proteinosomes, or coacervates. The vesicles may include surface functionalization to improve polynucleotide encapsulation and / or to bind polynucleotides having specific sequences. Encapsulation in vesicles provides protection for the polynucleotides. Additional protection is provided by addition of one or more stabilizers. The stabilizer may be nucleic-acid stabilizers that stabilize the polynucleotides or may be a protective structural layer around the vesicles such as a layer of silica. A process for stably storing polynucleotides in vesicles and a process for recovering stored polynucleotides from vesicles are both disclosed. The polynucleotides may be used for storage of digital information.

The invention belongs to the technical field of biological medicine, and particularly relates to a polypeptide degradation agent for targeted degradation of CD26 as well as a preparation method and application thereof, and the polypeptide degradation agent is obtained by coupling tetrapeptide and lenalidomide; the amino acid sequence of the tetrapeptide is any one of SEQ ID NO. 1 and SEQ ID NO. 2. The polypeptide degradation agent disclosed by the invention can be used for specifically degrading DPP4, namely CD26 in a targeted manner; however, isoenzymes DPP7, DPP8, DPP9 and the like of DPP4 cannot be degraded, and high selectivity of the isoenzymes DPP7, DPP8, DPP9 and the like is embodied. Besides, the polypeptide degradation agent is small in molecular weight, one end of the polypeptide degradation agent is a natural short peptide, the polypeptide degradation agent has good histocompatibility, target protein can be rapidly degraded within 6 hours, and good tissue permeability is embodied.

This invention provides extracellular protein degraders and compositions that have an asialoglycoprotein receptor (ASGPR) Binding Ligand bound to a specific Extracellular Protein Targeting Ligands for the selective degradation of the Target Extracellular Protein for example an immunoglobulin or other extracellular protein in vivo to treat disorders mediated by that protein.

The invention relates to a novel anthocyanidin O-methyltransferase (lmg 13579), a coding gene of the novel anthocyanidin O-methyltransferase and application of the novel anthocyanidin O-methyltransferase in catalyzing cyanidin-3-O-glucoside to generate paeoniflorin-3-O-glucoside. Gene cloning, prokaryotic expression and in-vitro enzyme activity verification prove that the enzyme can specifically catalyze methylation modification of anthocyanin B ring 3 '-hydroxyl sites. High-efficiency soluble expression is realized by using an escherichia coli heterologous expression system, and high-purity protein is obtained after His / GST double-tag purification. An in-vitro enzymatic reaction shows that after the reaction is carried out for 1 hour under the condition of 30 DEG C, a remarkable characteristic substance peak appears, and the substance is identified as paeoniflorin-3-O-glucoside (RT = 2.78) through LC-MS / MS (Liquid Chromatography-Mass Spectrometry / Mass Spectrometry). The discovery of the enzyme can improve the problems of low catalytic efficiency, low substrate binding specificity, unclear catalytic sites and the like of anthocyanin O-methyltransferase, and the molecular efficiency and specificity of synthesis of paeoniflorin-3-O-glucoside are improved by obtaining zymoprotein with definite functions and optimizing the catalytic process of anthocyanin methylation.

The invention discloses a method for predicting net energy requirements of piglets with different weaning weights, and belongs to the field of animal nutrition and feed science. According to the method, a factorial method is adopted, and the net energy demand amount of piglets is divided into a maintenance net energy demand amount, a protein deposition net energy demand amount and a fat deposition net energy demand amount. The method comprises the following steps: collecting data such as piglet body components, body weight, feed intake and the like, establishing a relation model of body weight, body protein and body fat deposition rate of 5-25kg piglets, determining energy contents of body protein and body fat, establishing a relation model of net energy demand, body weight, protein and fat deposition energy, and distinguishing model differences of piglets with high and low weaning weight. And dynamically predicting the net energy demand amount and the optimal feed net energy concentration in combination with the growth curve and the feed intake curve. According to the method, the feed utilization efficiency is improved, the cost is reduced, and a basis is provided for accurate feeding.

The application relates to the field of functional foods, and particularly discloses a high-digestion-absorption-rate energy bar suitable for an old sports crowd and a preparation method thereof. The high-digestion-absorption-rate energy bar suitable for the old sports crowd comprises the following raw materials in parts by weight: a graded protein system 20-28 parts, a fat compound 8-12 parts, a carbohydrate compound 40-50 parts, an intestinal regulation compound 3-6 parts, an electrolyte enhancer 1-2 parts, and a texture modifier. The graded protein system comprises a fast-absorption peptide segment, a slow-release peptide segment and an active synergistic component. The fast-absorption peptide segment is a deep hydrolysis whey peptide. The slow-release peptide segment is at least one selected from casein phosphopeptide, casein peptide, collagen peptide, pea peptide, egg white peptide and rice peptide. The active synergistic component is a small-molecule active peptide. The energy bar has the advantages of high protein digestion and absorption rate, precise adaptation to the sports needs of the old, friendliness to chewing and swallowing, high retention rate of nutritional components, high safety and high adaptability.