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Polynucleotide encapsulation and preservation using self-assembling membranes

A technology of polynucleotide and self-assembly, which is applied in the direction of non-active ingredient medical preparations, active ingredient-containing medical preparations, microcapsule preparations, etc., and can solve problems such as wear and tear

Active Publication Date: 2021-06-11
MICROSOFT TECH LICENSING LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Magnetic and optical media can wear out in five to ten years

Method used

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  • Polynucleotide encapsulation and preservation using self-assembling membranes
  • Polynucleotide encapsulation and preservation using self-assembling membranes
  • Polynucleotide encapsulation and preservation using self-assembling membranes

Examples

Experimental program
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Embodiment

[0078] In the following examples, "sample DNA" is a 150 bp double-stranded DNA molecule having an arbitrary sequence. Sample DNA was obtained from Integrated DNA Technologies (IDT), PCR amplified and purified using standard techniques. In these examples, each copy of the sample DNA has the same sequence, but in practical digital information storage implementations, there are many different sequences represented in the pool of DNA molecules.

[0079] Figure 5 is a fluorescence microscopy image of the aggregate-DNA conjugate. White or light spots on the image indicate co-localization of DNA within aggregates. Aggregates were formed from a mixture of poly-T oligonucleotides (ssDNA molecules with approximately 20 thymine bases) and poly-L-lysine (poly-K) obtained from IDT. The sample DNA was fluorescently labeled with 0.5 μL of 100 μM stock solution cyanine 5 (Cy-5) at its 5′ end. Cy5 is a fluorescent dye belonging to the Cyanine family of synthetic polymethine dyes. Cy5 is ...

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Abstract

Polynucleotides such as DNA are stored inside vesicles formed from self-assembling membranes. The vesicles may be protocells, liposome, micelles, colloidosomes, proteinosomes, or coacervates. The vesicles may include surface functionalization to improve polynucleotide encapsulation and / or to bind polynucleotides having specific sequences. Encapsulation in vesicles provides protection for the polynucleotides. Additional protection is provided by addition of one or more stabilizers. The stabilizer may be nucleic-acid stabilizers that stabilize the polynucleotides or may be a protective structural layer around the vesicles such as a layer of silica. A process for stably storing polynucleotides in vesicles and a process for recovering stored polynucleotides from vesicles are both disclosed. The polynucleotides may be used for storage of digital information.

Description

Background technique [0001] The volume of digital information is increasing at an exponential rate. This enormous increase in the amount of digital information has the potential to outstrip the capabilities of traditional storage technologies. One promising technology for storing large amounts of digital information is deoxyribonucleic acid (DNA). DNA is well known as a molecule that can store genetic information. However, DNA can also be used as a storage medium for digital information. Several different groups have successfully converted a computer file into a string of nucleotide bases, synthesized the DNA encoding the string, sequenced the synthetic DNA, and then recovered the original digital information with 100% accuracy . [0002] As a storage medium, DNA has potential advantages over conventional optical and magnetic media in terms of information density and stability. Using DNA for storage could achieve theoretical data densities of more than 200 petabytes per g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/02A61K9/127B01J13/10B01J13/22
CPCB01J13/22B01J13/10A61K47/6455A61K47/6923B01J13/04A61K47/6911A61K47/62A61K9/1271A61K9/1277A61K31/7088A61K47/14A61K47/183A61K47/26A61K47/36
Inventor K·施特劳斯B·H·阮
Owner MICROSOFT TECH LICENSING LLC
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