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46 results about "Eisosome assembly" patented technology
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The aggregation, arrangement and bonding together of a set of components to form an eisosome, a cell part that is composed of the eisosome membrane or MCC domain, a furrow-like plasma membrane sub-domain and associated integral transmembrane proteins, and the proteins (eisosome filaments) that form a scaffolding lattice on the cytoplasmic face. [GOC:al, GOC:jp, PMID:19564405]
Assemblies of Oligomeric Amyloid Beta Protein and Uses Thereof
InactiveUS20080044356A1Easy to removeDisruptionPeptide/protein ingredientsImmunoglobulins against animals/humansChemistryAmyloid beta proteins
Owner:RGT UNIV OF MINNESOTA
Production of proteins
A method for forming a fusion protein that is expressed as a recombinant protein body-like assembly in host eukaryotic cells and organisms other than higher plants as host systems is disclosed. More particularly, peptides and proteins are fused to protein sequences that mediate the induction of recombinant protein body-like assembly (RPBLA) formation, are stably expressed and accumulated in these host cells after transformation with an appropriate vector. Methods for preparing the fusion protein are also disclosed.
Owner:ERA BIOTECH SA
Prodrug activation compound, prodrug system, preparation method and application thereof
ActiveCN111718395AProdrug activation achievedTo achieve specific enrichmentOrganic active ingredientsPeptide preparation methodsPhosphorylationTyrosine
The invention provides a prodrug activation compound, which is characterized by comprising a polypeptide fragment, and a tetrazine group and a hydrophobic group which are connected to the polypeptidefragment through chemical bonds; wherein at least one phosphorylated tyrosine exists in the amino acid of the polypeptide fragment. The prodrug activation compound provided by the invention has targeting property and specificity, can be quickly and efficiently accumulated in tumor cells and is self-assembled in situ to form a nano assembly, so that a trans-cyclooctene modified anti-tumor prodrug is efficiently and specifically activated; the prodrug activating compound and the prodrug system are good in biocompatibility and free of system toxicity. A solid-phase synthesis method is adopted inthe preparation method of the prodrug activated compound, the operation is simple, and the obtained product is high in chemical purity and high in total yield.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Polypeptide polymer nanomaterial and preparing method and application thereof
ActiveCN107412782AGood biocompatibilityReduce neurotoxicityPowder deliveryNervous disorderDiseaseNeurotoxicity
The invention provides a polypeptide polymer nanomaterial and a preparing method and application thereof. The polypeptide polymer nanomaterial comprises chitosan, a polypeptide sequence for recognizing beta-amyloid protein and a polypeptide sequence for activating autophagy, and the polypeptide sequences are connected to the chitosan. A polypeptide polymer prepared through solid-phase synthesis and Michael addition has good biological compatibility and anti-A[beta] neurotoxicity, polypeptide polymer nanospheres obtained from the polypeptide polymer can be co-assembled with A[beta] so as to effectively prevent aggregation of A[beta] and reduce the neurotoxicity of A[beta], meanwhile, a co-assembly can activate autophagy after entering cells, A[beta] can be degraded through autophagy, so that Alzheimer's disease can be treated in a synergic mode, the Alzheimer's disease treatment efficiency of the polypeptide nanomaterial is improved greatly, and application prospects are broad.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Amphiphilic pillar [5] arene self-assembled vesicle and depolymerization reversibility and controllability control method thereof
InactiveCN103980122AAchieve self-assemblyImprove mechanical propertiesOrganic compound preparationCarboxylic acid esters preparationDepolymerizationSynthesis methods
The invention discloses a synthesis method of low-polyethylene-glycol functional amphiphilic pillar [5] arene compound (AP5-glycol), wherein AP5-glycol is self-assembled in water to form a vesicle, and the vesicle can generate responsive depolymerization (the vesicle becomes small or gradually disappears) after being affected by external physical stimulation such as heating, ultrasonic treatment, violent stirring and the like and can be rapidly formed again after the external stimulation is stopped, i.e., the two processes including vesicle formation and vesicle depolymerization are reversible and controllable. Therefore, the vesicle has excellent thermodynamic reversibility and controllability. In addition, KPF6 and benzo-18-crown-6 can be respectively used as a switch for depolymerizing and forming a vesicle self-assembly again. The characteristics can ensure that the vesicle has outstanding recyclability in application such as drug release or transfer and the like.
![Amphiphilic pillar [5] arene self-assembled vesicle and depolymerization reversibility and controllability control method thereof](https://images-eureka.patsnap.com/patent_img/606d4862-338b-48d2-b936-089ed3044858/140523135951.PNG "Amphiphilic pillar [5] arene self-assembled vesicle and depolymerization reversibility and controllability control method thereof")
![Amphiphilic pillar [5] arene self-assembled vesicle and depolymerization reversibility and controllability control method thereof](https://images-eureka.patsnap.com/patent_img/606d4862-338b-48d2-b936-089ed3044858/140523135957.PNG "Amphiphilic pillar [5] arene self-assembled vesicle and depolymerization reversibility and controllability control method thereof")
![Amphiphilic pillar [5] arene self-assembled vesicle and depolymerization reversibility and controllability control method thereof](https://images-eureka.patsnap.com/patent_img/606d4862-338b-48d2-b936-089ed3044858/140523140003.PNG "Amphiphilic pillar [5] arene self-assembled vesicle and depolymerization reversibility and controllability control method thereof")
Owner:NANTONG VOCATIONAL COLLEGE
Beta-casein assemblies for enrichment of food and beverages and methods of preparation thereof
ActiveUS20110038987A1Reducing system free energyDecrease in free energyProtein composition from fishMilk preparationCasein micellesNano size
The invention relates to a composition for the enrichment of food and / or beverage and to a method of preparing such composition. The composition comprises additive loaded beta-casein micelles which are of a diameter of about 100 nm or less. These nano-sized beta-casein assemblies are formed at pH values which are preferably one or more pH units above or below the pI of the protein (pI=5.3). More preferably the beta-casein nano-assemblies are formed at a pH range between about 6.0 and about 8, or between about 2.0 and about 4.2. The invention provides vehicles for delivery of additives via transparent beverages and other foods and drinks and / or acidic foods and drinks and / or non fat foods and drinks.
Owner:TECHNION RES & DEV FOUND LTD
Expression vector of nuclease protein Cas9 and construction method and expression purification of expression vector of nuclease protein Cas9
PendingCN110499305ALow costEasy to operatePolypeptide with localisation/targeting motifHydrolasesSequence signalEscherichia coli
The invention discloses an expression vector of a nuclease protein Cas9 and a construction method and expression purification of the expression vector of the nuclease protein Cas9. According to the expression vector of the nuclease protein Cas9, an original thioredoxin sequence in pET32a is replaced with cDNA of an amplified thioredoxin-histidine-tag-tobacco-etch-virus-protease recognition site, and codons are optimized to a Cas9 gene sequence which is easy to express in a host and has nuclear localization signal peptide and 3xFLAG tag sequence fused in a C-terminal to be integrated into the thioredox in sequence. According to the expression vector of the nuclease protein Cas9 and the construction method and expression purification of the expression vector of the nuclease protein Cas9, codon optimization and thioredoxin fusion expression are used for obtaining a large amount of the nuclease Cas9 protein, under the condition that only an LB medium is used, 10 mg of a highly purified active Cas9 protein can be finally obtained per gram of escherichia coli Tuner (DE3) wet bacteria, and high expression of the high-fidelity nuclease protein Cas9 at a low cost is realized; and the requirements on the host are not high, and the obtained protein can be used for large-scale biological in vitro assembly and in vivo gene editing experiments.
Owner:NANJING UNIV OF SCI & TECH
Production of proteins
InactiveUS8822181B2Readily obtainable and purifiableCalcitoninsUnicellular algaeEisosome assemblyProtein
A method for forming a fusion protein that is expressed as a recombinant protein body-like assembly in host eukaryotic cells and organisms other than higher plants as host systems is disclosed. More particularly, peptides and proteins are fused to protein sequences that mediate the induction of recombinant protein body-like assembly (RPBLA) formation, are stably expressed and accumulated in these host cells after transformation with an appropriate vector. Methods for preparing the fusion protein are also disclosed.
Owner:ERA BIOTECH SA
Fluorescent probe based on polyoxometallate and assembly of polyoxometallate and application of fluorescent probe in spermine detection
ActiveCN113583673AQuick responseSimple and fast operationFluorescence/phosphorescenceLuminescent compositionsFluoProbesFluorophore
The invention discloses a fluorescent probe based on polyoxometallate and an assembly thereof and an application of the fluorescent probe in spermine detection, and belongs to the technical field of fluorescent probes. According to the invention, a supramolecular assembly strategy is utilized, environment-sensitive polyoxometallate EuW10 is taken as a fluorophore, a peptide fragment GL-22 of HPV E6 is introduced to construct an assembly, and specific recognition and detection of spermine (Spm) are realized. The EuW10 / GL-22 assembly can be used for effectively distinguishing spermine (Spm) and spermidine (Spd), so that the specific response to the Spm is realized. The linear response range of the EuW10 / GL-22 assembly to spermine is 0.05 to 0.6 [mu] mol / L, and the detection limit is 2.2 nmol / L. The method is quick in response, high in sensitivity and strong in specificity in the aspect of detecting trace spermine biomarkers. Therefore, the detection performance of the probe can be effectively improved through a supramolecular assembly strategy, and high-sensitivity detection of the biogenic polyamine is realized.
Owner:JILIN UNIV
Nucleic acid assemblies for use in targeted delivery
PendingUS20210317479A1Useful physiochemical propertyEnhance stability and half-lifeSpecial deliveryActivity regulationCell organisationIn vivo
Disclosed are compositions and methods involving nucleic acid assemblies that enclose and / or protect cargo. Disclosed are compositions that include a nucleic acid assembly comprising one or more nucleic acid molecules and cargo comprising two or more cargo molecules. The nucleic acid assembly can have physiochemical properties that: (i) enhance targeting of the composition to one or more types of cells, tissues, organs, or microenvironments relative to other types of cells, tissues, organs, or microenvironments in vivo; (ii) enhance stability and / or half-life of the composition in vivo; and / or (iii) reduce immunogenicity of the composition. The nucleic acid assembly and / or cargo can have features that enhance intracellular trafficking of nucleic acid assembly and / or its cargo. The cargo can be enclosed and / or protected by the nucleic acid assembly. Some or all of the cargo molecules in the composition can be present in a defined stoichiometric ratio.
Owner:MASSACHUSETTS INST OF TECH +1
Nucleic acid assembly, vector, cell, methods and kit thereof
The present disclosure relates to a nucleic acid assembly (NAA), comprising sensor domain and handle domain; an assembly interfaceable motif (AIM) sequence optionally along with intracellular targeting motif (ITM) sequence; and an AIM-NAA complex. It also relates to a vector comprising assembly interfaceable motif sequence optionally along with intracellular targeting motif sequence and a cell comprising the vector. Further, the instant disclosure also provides a method to obtain the nucleic acid assembly, method of intracellular targeting and kit thereof.
Owner:NAT CENT FOR BIOLOGICAL SCI
Disordered protein-based seeds for molecular clustering
ActiveUS10538756B2Rapid and reversible clusteringEasy and rapid purificationAntibody mimetics/scaffoldsDepsipeptidesBio moleculesCell Aggregations
A system and method for reversibly controlling clustering of proteins around an engineered multivalent nucleus is disclosed. The system and method utilize clustering, which may be controlled by light activation or deactivation. The system and method enable the spatiotemporal control of protein supramolecular assemblies, including liquid-like droplets under some conditions, and solid-like gels under other conditions. The system and method can be utilized for segregating or locally concentrating desired proteins and / or RNA in cells or cell lysate, which may be useful for protein purification purposes, or for assembling single or multiple membraneless bodies within specific sub-regions of the cells. These synthetically assembled bodies may recruit both transgenic and endogenic proteins and other biomolecules, thus can be linked to affect and even trigger a plethora of cellular processes, including both physiological and pathological (e.g., protein aggregation) processes.
Owner:THE TRUSTEES FOR PRINCETON UNIV
Biosensor as well as preparation method and application
ActiveCN111812064AAchieving High Sensitivity DetectionHigh detection sensitivityPreparing sample for investigationScattering properties measurementsNanoparticleDna assembly
The invention discloses a biosensor as well as a preparation method and application thereof. The biosensor disclosed by the invention is an assembly comprising gold and silver core-shell nanoparticle-tetrahedral structure DNA containing azobenzene molecules. The preparation method of the biosensor comprises the following steps of: preparing the gold and silver core-shell nanoparticles; preparing tetrahedral structure DNA containing the azobenzene molecules; and (3) assembling the gold and silver core-shell nanoparticle-tetrahedron DNA assembly containing the azobenzene molecules. According tothe biosensor which is the assembly comprising the gold and silver core-shell nanoparticle-tetrahedral structure DNA containing azobenzene molecules, high-sensitivity detection of miR-21 can be achieved on the single-nanoparticle scale, the concentration of miR-21 and the red shift amount of a scattering spectrum of the gold-silver core-shell nanoparticle-tetrahedral structure DNA assembly containing the azobenzene molecules are in a linear relation, and important significance is achieved for diagnosis and prognosis treatment of early-stage cancer.
Owner:NANJING UNIV OF POSTS & TELECOMM
Strong oncolytic rod-shaped nano-assembly, preparation method and application thereof
PendingCN110665008APromote enrichmentIncrease intakePowder deliveryOrganic active ingredientsSubcellular organelleBlood drug
The present invention provides a strong oncolytic rod-shaped nano-assembly and a preparation method thereof. Oncolytic monomers of dendritic peptide molecules are constructed. The oncolytic monomers comprise chemotherapeutic drugs connected by sensitive bonds and residues peripherally modified with oncolytic effects, and the residues are covered with a negatively charged shielding layer; the oncolytic monomers are induced to self-assemble on inner cores and closely arranged on surfaces of the inner cores to form the rod-shaped nano-assembly; or the rod-shaped nano-assembly is directly stackedthrough an organic molecular design. The provided rod-shaped supramolecular assembly morphology affects biological behaviors of particles, has advantages of prolonging blood drug circulation time, increasing tumor enrichment, improving solid tumor penetration ability, increasing cell uptake and special subcellular organelle enrichment, etc., and greatly increases anti-tumor effects.
Owner:NANJING IND INST FOR ADVANCED INTELLIGENT EQUIP
Nucleic acid-based assembly and uses thereof
The present invention relates to a nucleic acid-based assembly comprising: at least one nucleic acid aptamer, and at least one nucleic acid motif designed to physically capture a drug. The nucleic acid motif may comprise one or more photo-responsive moieties that effect the release of the drug upon irradiation. The aptamer and the nucleic acid motif each can be covalently linked to one or more lipids, and the lipid-modified aptamer and nucleic acid motif may form the assembly through noncovalent interaction. The invention further relates to use of the nucleic acid-based assembly in the treatment of cancer.
Owner:UNIVERSITY OF BONN
LIPID ASSEMBLIES AND USES THEREOF AND SOME pH AND ELECTROSTATIC MODULATING LIPIDS TO BE USED IN SAID ASSEMBLIES
Provided are modified lipid compounds and their use in the formation of lipid assemblies. Also provided are lipid assemblies including (a) an amphoteric lipid including in covalent association with (i) one or more acyl chains; (ii) one or more weak base moiety; (iii) one or more weak acid moiety; the lipid assembly also including (b) one or more additional lipids, at least one of which being a zwitterionic lipid. In some embodiments, the amphoteric lipid is a compound including (a) a tri-functional moiety; (b) two non-phosphate lipid chains associated with two of the functional moieties of said tri-functional moiety; (c) optionally a spacer moiety associated with the third of the functional moieties of said tri-functional moiety; and (d) a polyalkylamine optionally including a short peptide with one to several carboxylic acid residues. Further provided are the uses of the lipid assembly, inter alia, for transfection or cancer treatment.
Owner:YISSUM RES DEV CO OF THE HEBREW UNIV OF JERUSALEM LTD
SERS biosensor and application thereof in preparation of detection system for detecting myocardial infarction miRNA
The invention relates to an SERS biosensor; hairpin probe DNA is modified on the surface of Fe3O4; MCH is added for incubation to prevent non-specific binding; miRNA, auxiliary chain DA and auxiliary chain DB are added to generate an enzyme digestion product; hairpin DNA 1 and hairpin DNA 2 are modified on the surface of AuNP in advance; the dispersed AuNP modified by the hairpin DNA 1 and the hairpin DNA 2 is added into the incubated Fe3O4 to carry out a hybrid chain amplification reaction; reaction is carried out overnight; and centrifugal washing is carried out. According to the invention, in a nano-assembly SERS detection system, for unreacted gold nano-cluster elements and aggregates generated by non-triggering, Fe3O4 is adopted as a magnetic separation substrate; an assembly triggered by hybrid chain amplification is rapidly separated out through surface functionalization and exclusion of non-specific adsorption, so that the aggregates only containing gold nanoparticles on the Fe3O4 surface are obtained; a very high SERS signal is achieved; and myocardial infarction miRNA detection is achieved.
Owner:ANHUI UNIVERSITY OF TECHNOLOGY
Stable nanoscale nucleic acid assemblies and methods thereof
Methods for the top-down design of nucleic acid nanostructures of arbitrary geometry based on target shape of spherical or non-spherical topology are described. The methods facilitate 3D molecular programming of lipids, proteins, sugars, and RNAs based on a DNA scaffold of arbitrary 2D or 3D shape. Geometric objects are rendered as node-edge networks of parallel nucleic acid duplexes, and a nucleic acid scaffold routed throughout the network using a spanning tree formula. Nucleic acid nanostructures produced according to top-down design methods are also described. In some embodiments, the nanostructures include single-stranded nucleic acid scaffold, DX crossovers, and staple strands. In other embodiments, the nanostructures include single-stranded nucleic acid scaffold, PX crossovers and no staples. Modified nanostructures include chemically modified nucleotides and conjugated to other molecules are described.
Owner:MASSACHUSETTS INST OF TECH
A method for the simultaneous detection of two miRNAs based on the FRET effect
ActiveCN108037100BUniform structureGood biocompatibilityMicrobiological testing/measurementBiocompatibility TestingBiology
A method for simultaneously detecting two types of miRNAs on the basis of FRET effects, relating to the field of material chemistry. According to the method, a gold rod nucleus-upconversion satellite-shaped structure assembly is synthesized, a standard curve is built, a golden rod nucleus-upconversion satellite-shaped nanocrystal assembly with a uniform structure and good biocompatibility is finally prepared, and a platform capable of implementing quantitative detection of two types of intracellular miRNAs on the basis of FRET effects is provided.
Owner:JIANGNAN UNIV +1
Self-Assembling Protein Nanostructures Displaying Paramyxovirus and/or Pneumovirus F Proteins and Their Use
Disclosed herein are nanostructures and their use, where the nanostructures include a plurality of first assemblies, each first assembly comprising a plurality of identical first polypeptides selected from 153_dn5A, 153_dn5A.1 and I53_dn5A.2, or variants thereof; and a plurality of second assemblies, each second assembly comprising a plurality of identical second polypeptides being 153 dn5B or a variant thereof, wherein the plurality of first assemblies non-covalently interact with the plurality of second assemblies to form a nanostructure; and wherein the nanostructure displays multiple copies of one or more paramyxovirus and / or pneumovirus F proteins, or antigenic fragments thereof.
Owner:UNIV OF WASHINGTON
A kind of cisplatin ligand and its application in the preparation of tumor nano-diagnosis and treatment agent
ActiveCN112704685BInhibit dense packingIncreased ability to produce singlet oxygen by lightMaterial nanotechnologyEnergy modified materialsCisplatinDiagnostic agent
The invention discloses a cisplatin ligand and its application in preparing tumor nano-diagnosis and treatment agents, belonging to the technical field of medicine. The fluorescent unit and the singlet oxygen-generating unit are connected to one molecule through metal coordination, and the exterior of the assembly is protected by a glycol chain to make it stable in the body circulation process. At the same time, the metal coordination inhibits the interaction between porphyrins The dense accumulation of the light greatly improves its ability to generate singlet oxygen, thereby improving the anti-tumor efficiency.
Owner:NANTONG UNIVERSITY
Method for determining minimum entrapment binding ratio in polypeptide and siRNA co-assembly
PendingCN114660160APromote the progress of internal and external experimentsEasy to operateMaterial analysis by electric/magnetic meansElectrophoresesNanoparticle
The invention belongs to the field of bioengineering, and particularly relates to a method for determining the minimum entrapment binding ratio in a polypeptide and siRNA co-assembly. The invention discloses a method for determining the minimum entrapment binding ratio in a polypeptide and siRNA co-assembly. The method comprises the following steps: S1, preparing 2% agarose gel; s2, preparing the CPP-siRNA nano particles; s3, target polypeptide is added, an electrophoresis experiment is carried out, and a gel imager is used for imaging; and observing a map in an imaging result by naked eyes, selecting a group with no tailing of a protein electrophoresis track in the image, and taking the minimum binding ratio in the group without tailing as the minimum entrapment binding ratio. The invention discovers a method for determining the minimum entrapment binding ratio of the positively charged nanoparticles formed by self-assembly of the polypeptide and the siRNA, discovers several polypeptide sequences suitable for the method, and fills the blank in the prior art. The method is simple to operate, and the judgment method is very visual and easy to operate.
Owner:陈璞
Toxoplasma gondii vaccines and their use
InactiveUS20190282679A1Protozoa antigen ingredientsAntibody mimetics/scaffoldsGondii toxoplasmaNucleotide
Disclosed herein are polynucleotides encoding multi-epitope polypeptides and assemblies thereof, and their use for treating or limiting Toxoplasma gondii infection.
Owner:MASSACHUSETTS INST OF TECH +1
Blood Typing Instructional System
This teaching tool can include: a base having an A base opening, a B base opening, and a Rh base opening; a set of antibody assemblies including; a blood cell model having an set of openings for receiving at least one of a set of antigen assemblies wherein when the A antigen head of the blood cell antigen assembly is received into the A assembly, A agglutinate is represented, wherein the B antigen head of the blood cell antigen assembly is received into the B assembly, B agglutinate is represented, and wherein the Rh antigen head of the blood cell antigen assembly is received into the Rh assembly, Rh agglutinate is represented; and, a capillary representation defining a capillary cavity for comparison with A, B, or Rh agglutinated representations to determine if the A, B or Rh agglutinated can be received in the capillary representation.
Owner:MALACHOWSKY KENNETH J
Fluorescent probes based on polyoxometalates and their assemblies and their application in the detection of spermine
ActiveCN113583673BQuick responseSimple and fast operationFluorescence/phosphorescenceLuminescent compositionsFluoProbesFluorophore
A fluorescent probe based on polyoxometalate and its assembly and its application in detecting spermine belongs to the technical field of fluorescent probes. The present invention utilizes a supramolecular assembly strategy to synthesize the environment-sensitive polyoxometalate EuW 10 As a fluorophore, the assembly was constructed by introducing a peptide GL‑22 of HPV E6, and the specific recognition and detection of spermine (Spm) was realized. wxya 10 The / GL‑22 assembly efficiently discriminates spermine (Spm) from spermidine (Spd), enabling a specific response to Spm. wxya 10 The linear response range of / GL‑22 assembly to spermine was 0.05-0.6 μmol / L, and the detection limit was 2.2 nmol / L. The method has the advantages of rapid response, high sensitivity and strong specificity in the detection of trace spermine biomarkers. Therefore, the supramolecular assembly strategy can effectively improve the detection performance of the probe and realize the highly sensitive detection of biologically derived polyamines.
Owner:JILIN UNIV
Compositions and methods for protein expression and delivery
Owner:NAT UNIV OF SINGAPORE
Fusion protein based on single-chain antibody fragment, nano assembly and preparation method and application thereof
ActiveCN114516921AFast preparationStable manufacturingPowder deliveryPeptide/protein ingredientsPolyesterSingle-Chain Antibodies
The invention relates to a recombinant fusion protein, a nano assembly as well as a preparation method and application of the nano assembly. The nano assembly is constructed from at least one recombinant fusion protein, hydrophobic degradable polyester and derivatives thereof. The recombinant fusion protein comprises a single-chain antibody fragment, a connecting peptide and albumin with a hydrophobic region, wherein the single-chain antibody fragment has at least one of a competitive binding target, an inhibition signal channel, an activation signal channel and an antigen targeting effect. The invention also discloses a preparation method of the monoclonal antibody fragment-albumin recombinant fusion protein and a construction method of a nano assembly. The nano assembly provided by the invention provides a brand-new construction method of a monospecific, bispecific or multispecific antibody, and the nano assembly can be used for treating diseases such as tumors, autoimmune diseases and the like.
Owner:SOUTH CHINA UNIV OF TECH
A nucleic acid delivery carrier and its preparation method and application
ActiveCN107556493BImprove delivery efficiencySilent efficiency is highPharmaceutical non-active ingredientsSerum freeHydrophobe
The invention provides a nucleic acid delivery carrier, a preparation method and application of the nucleic acid delivery carrier. The nucleic acid delivery carrier is a nanometer self-assembled bodyof an amphiphilic fluorinated material; and the amphiphilic fluorinated material is perfluorooctane-substituted oligomeric polyethyleneimine. According to the nucleic acid delivery carrier provided bythe invention, oligomeric polyethyleneimine and the perfluorooctane material are bonded together, and due to strong hydrophobicity of the perfluor material, not only can the nucleic acid compressingcapability of oligomeric polyethyleneimine be improved, but also the efficiency of cell endocytosis and endosome escape can be improved; positive charges of oligomeric polyethyleneimine not only can be used for electrostatic composition of siRNA, but also can utilize proton sponge effect to promote the endosome escape in the system, and by the combination of electrostatic composition of siRNA andthe endosome escape, the nucleic acid delivery carrier not only has very-high silence efficiency under the serum-free condition, but also can show very high nucleic acid delivery efficiency in the presence of serum, and is expected to be applied in gene therapy based on siRNA or DNA.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
A construction method and application of an aptamer-DNA polymer based on nonlinear hybridization chain amplification
ActiveCN109295133BAchieve therapeutic effectAchieve specific targetingOrganic active ingredientsMicrobiological testing/measurementAptamerPhosphorylation
The invention discloses the construction and application of a DNA macromolecular polymer based on non-linear hybridization chain amplification. The system introduces an initiating chain into the system, and induces six different non-transforming Clip-like nucleic acid probes cascade self-assembled to form DNA polymers. On this basis, the 5' end of the partially assembled sequence was treated with phosphorylase to form a more stable DNA assembly under the action of ligase. Nucleic acid aptamers are bound to its sticky ends to identify target cells and introduce cytotoxic drug doxorubicin to realize imaging and drug delivery to target cells.
Owner:FUZHOU UNIV
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