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Method of harmless extracting living body bee DNA

A bee and living technology, applied in the field of non-harmful extraction of living bee DNA, can solve the problems of harming living bees, affecting production performance and examining the value of queen bee species, achieving the effects of low cost, improved efficiency and simple operation process

Inactive Publication Date: 2008-09-03
ZHEJIANG UNIV
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Problems solved by technology

[0009] The above-mentioned bee DNA extraction either kills individual bees, or cuts off some tissues and organs of individual bees. Individual bees are very small. Such treatment will inevitably harm living bees, affecting their production performance and the use of queen bee drones. value

Method used

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  • Method of harmless extracting living body bee DNA
  • Method of harmless extracting living body bee DNA
  • Method of harmless extracting living body bee DNA

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Embodiment 1

[0022] In order to confirm that the DNA extracted from the muscle and molting of the same individual is the same, we used 4 pairs of microsatellite primers to detect the DNA extracted from the muscle and molting of 5 worker bees, 5 queen bees and 3 drones respectively.

[0023] 1) Collection of honeybee muscle and molted skin In the experimental apiary of Zhejiang University, the Italian bee variety "Zhe Nong Da No. 1" was selected as the experimental material, and 20 king bees were cultivated with the king-raising frame. The king box is placed in an incubator at 35°C and a relative humidity of 65% to 75% for cultivation; at the same time, 18-day-old sealed drone spleen and worker honeycomb are selected from the bee colony and placed in an incubator at 35°C and a relative humidity of 65% to 75%. Cultured in an incubator. Observe the development of queen bees, worker bees, and drones every day. When the queen bees, drones, or worker bees have just emerged, capture 5 queen bees,...

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Abstract

The present invention discloses a method for noninjuriously extracting living body honey bee DNA. It is characterized by that the DNA can be extracted from desquamate tissue of queen bee and spado or drone which is remained in the bottom portion of cell of bees. Said method is simple in operation process and does not injure living body honey bee. The invented noninjurious extraction method of living body honey bee DNA has important application value for breeding honey bee.

Description

technical field [0001] The invention relates to a method for extracting living honeybee DNA without damage. Background technique [0002] Bee breeding is to continuously expand, accumulate and strengthen a certain beneficial variation in the bee population due to natural mutation or gene recombination through selection and reproduction according to the characteristics of bee inheritance and variation, and to make this beneficial variation move towards the same variation A breeding method for the development of nature. Therefore, it is the utilization of the phenomenon of spontaneous mutation or gene recombination existing in nature. Among the existing bee varieties, a bee colony with a beneficial variation caused by spontaneous mutation or genetic recombination is selected, and it is specially bred into a system. Through continuous selection, mating is strictly controlled, and the same breeding goal is achieved. selection and breeding. In this way, after several generatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/10C12N15/12
Inventor 苏松坤蔡芳杜宏沪陈盛禄
Owner ZHEJIANG UNIV
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