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Method for extracting fermented grain RNA rapidly and efficiently

A high-efficiency, fermented grain technology, applied in the biological field, can solve the problems of low quality of total RNA, unsatisfactory extraction effect, unsatisfactory effect, etc., and achieve the effect of convenient operation, fast and efficient extraction, and little harm to human body.

Inactive Publication Date: 2019-12-20
BEIJING SHUNXIN AGRICULTURE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extraction effect of the above method is not ideal, the extraction time is shorter, and the cost is higher
In some RNA extraction methods, even if acidic phenol extraction is used to remove protein and DNA, the effect is not satisfactory, and the quality of total RNA extracted is not high.

Method used

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  • Method for extracting fermented grain RNA rapidly and efficiently

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Utilize the method of the present invention to ferment microbial community structure and its succession law in Niulanshan Erguotou. During the establishment of this method, a pre-test was carried out in advance. According to the fermentation process of Niulanshan Erguotou, the sampling positions were the center and edge positions of the upper, middle and lower ground tanks, and the 0, 3, 5, 7, 9, 13, On 19, 23, and 28, 300g of large-stubble fermented wine grains samples were taken. The sampling location was 50cm from the center of the fermentation tank to the bottom of the tank. The large-stubble fermented wine grains samples were mixed in a sterile sealed bag and sent to the laboratory for standby within 10 minutes. .

[0040] A method for rapidly and efficiently extracting RNA from fermented grains, comprising the steps of:

[0041] Step 1: Pretreatment of large stubble fermented wine grains samples:

[0042] (1) Weigh 1g of large stubble fermented wine grains and s...

Embodiment 2

[0069] The method of the present invention is used to analyze the structure and succession law of the microbial community of Niulanshan Erguotou fermentation. During the establishment of this method, a pre-test was carried out in advance. According to the fermentation process of Niulanshan Erguotou, the sampling positions were the center and edge positions of the upper, middle and lower ground tanks, and the 0, 3, 5, 7, 9, 13, At 19, 23, and 28, the second batch of fermented wine grains sample is 300g. The sampling location is 50cm from the center of the fermentation tank to the bottom of the tank. The second crop of fermented wine grains samples are mixed in a sterile sealed bag and sent to the laboratory within 10 minutes for standby .

[0070] A method for rapidly and efficiently extracting RNA from fermented grains, comprising the steps of:

[0071] Step 1: Pretreatment of fermented fermented grains samples for the second crop:

[0072] (1) Weigh 1g of the fermented ferm...

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Abstract

The invention relates to the field of biotechnology, in particular to a method for extracting fermented grain RNA rapidly and efficiently. The method comprises three steps of pretreating a fermented grain sample, extracting the total RNA of the fermented grain sample and removing the DNA from the total RNA of the sample. The method provided by the invention has the following beneficial effects: the fact that the microbial total RNA is extracted by a hot phenol method in a Baijiu making process is proposed for the first time; by the pretreatment step, microorganisms in the fermented grains anddistiller's yeasts can be obtained comprehensively; saccharose-containing buffer liquid is adopted in the extraction step, so high-quality RNA can be obtained; and 1 / 10 volume of 3 mol / L sodium acetate with the pH being 5.2 is added before acid phenol extraction, so RNA and protein layering is benefited. The method provided by the invention is low in cost, convenient and rapid in operation, avoidsenvironmental pollution and has small harm to a human body; furthermore, the RNA can be extracted from the fermented grains rapidly and efficiently, and the method is of great importance for researchon microbial total RNA extraction in the Baijiu making process.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for rapidly and efficiently extracting RNA from fermented grains. Background technique [0002] The number of microorganisms in liquor brewing is huge, and the fermentation components are extremely complex. At present, more than a thousand chemical substances have been detected, and most of these substances come from raw materials or microbial metabolism during fermentation. Fermented grains are an important source of flavor substances in the fermentation process, so fermented grains are the carrier of new flavor substances. In recent years, domestic liquor companies or scientific research institutes of universities have continuously explored and discovered flavor substances in liquor, hoping to discover new functional factors. Currently, new flavor substances discovered in the brewing process of liquor have complicated technical operations and heavy workload. , high equipm...

Claims

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Application Information

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IPC IPC(8): C12N15/10
CPCC12N15/1003
Inventor 魏金旺刘钢柳旭王瑛郝文军于晓涛
Owner BEIJING SHUNXIN AGRICULTURE CO LTD
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