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Polymorphic primers of cinnamomum camphora chloroplast SNP molecular markers and application of polymorphic primers

A polymorphic primer and molecular marker technology, applied in recombinant DNA technology, microbial determination/inspection, biochemical equipment and methods, etc. Poor repeatability and other problems, to achieve good repeatability

Active Publication Date: 2020-02-07
NANJING FORESTRY UNIV +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] For the deficiencies in the prior art, the purpose of the present invention is to provide polymorphic primers of camphor chloroplast SNP molecular markers, in order to solve the co-dominant DNA markers such as RAPD, ISSR used in the prior art, poor repeatability, polymorphism Another object of the present invention is to provide the application of polymorphic primers of Cinnamomum camphora chloroplast SNP molecular markers in the analysis of Cinnamomum camphora genetic diversity and Cinnamomum camphora pedigree analysis, in order to solve the problem that the prior art is difficult to comprehensively reveal Cinnamomum camphora. The question of the true level of Cinnamomum camphora genetic diversity

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  • Polymorphic primers of cinnamomum camphora chloroplast SNP molecular markers and application of polymorphic primers

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Embodiment 1

[0040] 1) Test material

[0041] The test materials come from 18 groups in the natural distribution area of ​​Cinnamomum camphora, namely Luzhou in Sichuan, Jing'an in Jiangxi, Ningdu in Jiangxi, Yibin in Sichuan, Daozhen in Guizhou, Guilin in Guangxi, Enjiang in Jiangxi, Longgang in Jiangxi, Anqing in Anhui, Le'an in Jiangxi, Ruihong in Jiangxi, Pingxiang in Jiangxi, Guangzhou in Guangdong, Nanchang in Jiangxi, Guiyang in Guizhou, Libo in Guizhou, Wuyishan in Fujian and Nanjing in Jiangsu. Ancient camphor trees with a breast diameter greater than 60 cm were selected, and 5-10 fresh leaves were collected from each individual and transported on dry ice, quickly frozen in liquid nitrogen and stored in a -80°C refrigerator. The specific location, latitude and longitude, and number of samples of the collection group are shown in Table 1.

[0042] Table 1 Sampling information of 18 ancient camphor populations

[0043] source sample size group longitude latitude ...

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Abstract

The invention discloses polymorphic primers of cinnamomum camphora chloroplast SNP molecular markers and application of the polymorphic primers, and belongs to the technical field of forestry molecular biology. 11 pairs of polymorphic primers of the cinnamomum camphora chloroplast SNP molecular markers are screened and applied to ancient cinnamomum camphora genetic diversity analysis and ancient cinnamomum camphora pedigree analysis, and on the basis of chloroplast haplotype analysis, results are shown that different haplotypes have obvious distribution differences, most haplotypes only existin specific populations, and only few haplotypes are shared haplotypes; and thus a fact is indicated that certain geographic differentiation exists among the haplotypes. A genetic differentiation coefficient Fst of 18 cinnamomum camphora populations is 0.212, gene flow Nm of the cinnamomum camphora populations is 0.929, and a result is shown that middle gene flow exists among the cinnamomum camphora populations. By adopting the polymorphic primers, the SNP molecular markers have good repeatability, a true level of cinnamomum camphora genetic diversity can be revealed completely, and theoretical foundations are provided for wild cinnamomum camphora resource protection and utilization.

Description

technical field [0001] The invention belongs to the technical field of forestry molecular biology, and in particular relates to polymorphic primers for camphor chloroplast SNP molecular markers and applications thereof. Background technique [0002] Cinnamomum camphora (L.) Presl. is a tall tree of the genus Cinnamomum camphora in the family Lauraceae. It has important ecological, cultural and economic values, and is an important timber and special economic tree species in my country. In recent years, there have been many reports on the taxonomy, physiology, chemical composition and utilization of Cinnamomum camphora, breeding of improved varieties and silviculture techniques, but the research on the genetic diversity of its germplasm resources is very insufficient. Cinnamomum camphora has an early cultivation history in our country, and the introduction and cultivation of camphor in different regions is frequent. It has continued to this day, and the origin of samples for p...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6895C12Q2600/156C12Q2531/113C12Q2527/107
Inventor 杨茂霞胥猛钟永达郦芝汀
Owner NANJING FORESTRY UNIV
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