Polymorphic primers for chloroplast SNP molecular markers of Cinnamomum camphora and its application
A polymorphic primer and molecular marker technology, applied in recombinant DNA technology, biochemical equipment and methods, microbial determination/inspection, etc., can solve the problem of low polymorphism, difficult to fully reveal the true level of the genetic diversity of camphor Poor repeatability, etc.
Active Publication Date: 2020-10-30
NANJING FORESTRY UNIV +1
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Problems solved by technology
[0004] For the deficiencies in the prior art, the purpose of the present invention is to provide polymorphic primers of camphor chloroplast SNP molecular markers, in order to solve the co-dominant DNA markers such as RAPD, ISSR used in the prior art, poor repeatability, polymorphism Another object of the present invention is to provide the application of polymorphic primers of Cinnamomum camphora chloroplast SNP molecular markers in the analysis of Cinnamomum camphora genetic diversity and Cinnamomum camphora pedigree analysis, in order to solve the problem that the prior art is difficult to comprehensively reveal Cinnamomum camphora. The question of the true level of Cinnamomum camphora genetic diversity
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Embodiment 1
[0040] 1) Test materials
[0041] The test materials came from 18 groups in the natural distribution area of Cinnamomum camphora, namely Luzhou in Sichuan, Jing'an in Jiangxi, Ningdu in Jiangxi, Yibin in Sichuan, Daozhen in Guizhou, Guilin in Guangxi, Enjiang in Jiangxi, Longgang in Jiangxi, Anqing in Anqing, Lean in Jiangxi, Ruihong in Jiangxi, Pingxiang in Jiangxi, Guangzhou in Guangdong, Nanchang in Jiangxi, Guiyang in Guizhou, Libo in Guizhou, Wuyishan in Fujian and Nanjing in Jiangsu. Choose ancient camphor trees with a diameter at breast height greater than 60cm, and each individual collects 5-10 pieces of fresh leaves and transports them on dry ice, and stores them in a refrigerator at -80℃ by quick freezing with liquid nitrogen. See Table 1 for the location, latitude and longitude, and number of samples of the specific collection group.
[0042] Table 1 Sampling information of 18 ancient camphor groups
[0043] source Sample size group longitude latitude Luzhou, Sic...
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The invention discloses polymorphic primers for camphor chloroplast SNP molecular markers and applications thereof, and belongs to the technical field of forestry molecular biology. The present invention screened 11 pairs of polymorphic primers of Cinnamomum camphora chloroplast SNP molecular markers, and applied them in the analysis of genetic diversity and pedigree of Cinnamomum camphora, based on the analysis of chloroplast haplotypes, the distribution of different haplotypes is significantly different , most of the haplotypes only exist in specific populations, and only a few haplotypes are shared haplotypes, indicating that there is a certain geographical differentiation among haplotypes. The genetic differentiation coefficient Fst of the 18 C. camphora populations is 0.212, and the gene flow Nm is 0.929, indicating that there is a moderate gene flow among C. camphora populations. The SNP molecular marker of the invention has good repeatability, can comprehensively reveal the true level of genetic diversity of camphor, and provides a theoretical basis for the protection and utilization of wild camphor resources.
Description
Technical field [0001] The present invention belongs to the technical field of forestry molecular biology, and specifically relates to a polymorphic primer for Cinnamomum camphora chloroplast SNP molecular marker and its application. Background technique [0002] Cinnamomum camphora (L.) Presl. is a tall tree of the genus Cinnamomum camphora in the Lauraceae family. It has important ecological, cultural and economic value. It is an important timber and special economic tree species in my country. In recent years, there have been many reports on the taxonomy, physiology, chemical composition and utilization, breeding and forest management of Cinnamomum camphora, but the research on the genetic diversity of its germplasm resources is very insufficient. Cinnamomum camphora has an earlier cultivation history in my country, and the introduction and cultivation are frequent between regions. It continues to this day, and the origin of samples for population genetic analysis is quite dif...
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IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6895C12Q2600/156C12Q2531/113C12Q2527/107
Inventor 杨茂霞胥猛钟永达郦芝汀
Owner NANJING FORESTRY UNIV
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