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SSR (Simple Sequence Repeat) molecular marker for genetic diversity analysis of clematis nepalensis and application of SSR molecular marker

A DNA molecule, 2-R technology, applied in the direction of sequence analysis, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as difficulty

Active Publication Date: 2022-05-06
北京市园林绿化科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since there is no information related to genome sequencing of Clematis maple leaf, it is difficult to develop its related SSR molecular markers

Method used

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  • SSR (Simple Sequence Repeat) molecular marker for genetic diversity analysis of clematis nepalensis and application of SSR molecular marker
  • SSR (Simple Sequence Repeat) molecular marker for genetic diversity analysis of clematis nepalensis and application of SSR molecular marker
  • SSR (Simple Sequence Repeat) molecular marker for genetic diversity analysis of clematis nepalensis and application of SSR molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0152] Example 1, Screening of Clematis maple leaf SSR molecular markers

[0153] 1. Test materials and methods

[0154] 1. Test material

[0155] Clematis maple leaf is mainly distributed in Beijing area, and occasionally distributed in Hebei and Henan. According to the field investigation results, the following nine locations were selected for sampling: Sibeiyu (Hebei), Gougezhuang (Hebei), Fangshan Sandu (Beijing), Wuheer Tunnel (Beijing), Yuntai Mountain (Henan), Nanshi Yangyang Grand Canyon (Beijing), Jingxi Ancient Road (Beijing), Xiayunling (Beijing), and Xinghuang (Beijing), the sampling standard is to sample 10 plants for each population, and the distance between individual plants should be at least 2m. Avoid taking samples from the same cliff crevice s material. The relevant information and numbers of all test materials are shown in Table 1.

[0156] Table 1. Basic information of population sampling

[0157] group number Place Number of samples S...

Embodiment 2

[0181] Example 2. Using SSR molecular markers to analyze the genetic diversity of Clematis maple leaf

[0182] 1. Genetic diversity parameters of 29 SSR loci

[0183] Observed allele number (Na), effective allele number (Ne), Shannon's diversity index (I), observed heterozygosity (Ho), and expectation of 29 SSR loci using population genetics software POPGENE Version1.32 Heterozygosity (He) and F statistics (Fit, Fis, Fst) were calculated. Polymorphism information content (PIC) was calculated using PowerMarker V3.25 software. Gene flow (Num)=(1-Fst) / 4Fst was calculated.

[0184] The results are shown in Table 5. It can be seen from Table 5 that in the 9 populations, the observed allele numbers (Na) of the 29 SSR loci varied from 2 to 4, with an average value of 2.4483. The effective allele number (Ne) ranged from 1.1172 to 2.2839, with an average of 1.6310. Shannon's diversity index (I) ranged from 0.2146 to 1.0092, with an average value of 0.5682; the primer with the highe...

Embodiment 3

[0193] Example 3. Using SSR molecular markers to calculate the genetic differentiation coefficient Fst among 9 Clematis maple leaf populations

[0194] The genetic differentiation factor (Fst) is an indicator of the degree of differentiation between populations and varies between 0 and 1. Fst less than 0.05 indicates that there is basically no genetic differentiation trend between the two populations, Fst between 0.05 and 0.15 indicates a moderate degree of differentiation, Fst between 0.15 and 0.25 indicates a large degree of genetic differentiation, and Fst greater than 0.25 indicates the degree of differentiation very big. The genetic differentiation coefficients of nine Clematis maple populations were calculated using GenALEx 6.5.

[0195] The results are shown in Table 7. It can be seen from Table 7 that the genetic differentiation coefficient (Fst) among the nine Clematis maple leaf populations varies from 0.023 to 0.469. The genetic differentiation coefficient (Fst) o...

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Abstract

The invention discloses an SSR (Simple Sequence Repeat) molecular marker for genetic diversity analysis of clematis nepalensis and application of the SSR molecular marker. The invention also discloses an SSR primer group for genetic diversity analysis of clematis malaytea, and the SSR primer group consists of a primer pair 1-a primer pair 29. The SSR primer group has any one of the following functions of n1)-n5): n1) analyzing or evaluating genetic diversity of clematis malaytea; n2) analyzing or evaluating the genetic differentiation degree of the clematis nepalensis; n3) analyzing or evaluating the genetic relationship of the clematis malaytea; n4) constructing or preparing a heredity map or fingerprint spectrum of the clematis nepalensis; and n5) identifying or distinguishing different geographical populations of clematis maple. Sequencing results of simplified genomes are used for splicing, the SSR molecular marker suitable for research on the genetic diversity of the clematis nepalensis is screened out, and the SSR molecular marker has important significance on development of the research on the genetic diversity of the clematis nepalensis and conservation work.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an SSR molecular marker for analyzing the genetic diversity of Clematis maple leaf and its application. Background technique [0002] Clematis acerifolia Maxim. is a plant of the genus Clematis in the family Ranunculaceae. The flowering period is April, and the flowers are elegant. They are mainly distributed in Fangshan, Mentougou and other places in Beijing, and occasionally in Hebei and Henan. Due to climate change, economic development and changes in its own genetic characteristics, the population of clematis maple leaf presents the problems of small number of plants, narrow distribution, slow population self-renewal and slow reproduction, and belongs to the first-class protected plants in Beijing. [0003] Genetic diversity refers to the sum of genetic variation within and between populations. Genetic diversity reflects the resistance of plants to adverse environme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11G16B30/10
CPCC12Q1/6895C12Q1/6858G16B30/10C12Q2600/156C12Q2531/113C12Q2565/125C12Q2535/122Y02A50/30
Inventor 赵正楠赵世伟张红伟杨媛李进宇陈晓吉乃喆冯慧
Owner 北京市园林绿化科学研究院
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