Camphor tree whole genome ssr molecular marker and its preparation method and application

Abstract

The invention discloses a whole genome SSR molecular marker of camphor tree and its preparation method and application. The camphor tree microsatellite molecular markers developed by the present invention can be applied to the population genetic diversity detection and kinship analysis of ancient camphor trees, providing a new batch of microsatellite molecular markers for Lauraceae plants, and also for the population genetic differentiation of Lauraceae plants New tools are provided for the study of genetic diversity and structure, the level of genetic diversity of populations, the gene flow and mating system between populations, species evolution, and molecular assisted breeding.

Description

Technical field: [0001] The invention belongs to the technical field of forestry molecular biology, and specifically relates to camphor tree molecular marker technology, and more specifically relates to a camphor tree whole-genome SSR molecular marker and its preparation method and its application in the analysis of ancient camphor genetic diversity and identification of kinship. Background technique: [0002] Microsatellite (Microsatellite) is also called short tandem repeats (Short Tandem Repeats, STRs), simple sequence repeats (Simple Sequence Repeat, SSRs), simple sequence length polymorphism (SSLP). It refers to a DNA sequence that is repeated in tandem multiple times in units of 1 to 6 nucleotides. It is commonly found in the genomes of eukaryotes and prokaryotes, and even in virus genomes. SSR molecular markers have the following characteristics: repeat units and repeat numbers are highly variable, polymorphic information capacity is high; co-dominant, following Mende...

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Problems solved by technology

However, compared with other species such as rice, corn, grapes, etc., the EST data of camphor tree included in the database of the National Center for Biotechnology Information (NCBI) is quite lacking, which is difficult to meet the needs of camphor tree EST-SSR marker development, and cannot be used for the development of camphor tree EST-SSR markers. DNA molecular marker development research provides sufficient information

This also greatly limits the development of camphor tree molecular biology research

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