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Camphor tree whole genome ssr molecular marker and its preparation method and application

A molecular marker and genome-wide technology, applied in biochemical equipment and methods, recombinant DNA technology, DNA/RNA fragments, etc., can solve the problem of restricting the research and development of camphor tree molecular biology, difficult to meet the needs of camphor tree EST-SSR marker development, Problems such as lack of camphor tree EST data

Active Publication Date: 2022-01-18
JIANGXI ACAD OF FORESTRY
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  • Abstract
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Problems solved by technology

However, compared with other species such as rice, corn, grapes, etc., the EST data of camphor tree included in the database of the National Center for Biotechnology Information (NCBI) is quite lacking, which is difficult to meet the needs of camphor tree EST-SSR marker development, and cannot be used for the development of camphor tree EST-SSR markers. DNA molecular marker development research provides sufficient information
This also greatly limits the development of camphor tree molecular biology research

Method used

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  • Camphor tree whole genome ssr molecular marker and its preparation method and application
  • Camphor tree whole genome ssr molecular marker and its preparation method and application
  • Camphor tree whole genome ssr molecular marker and its preparation method and application

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Embodiment 1

[0077] One, the preparation method of the primer based on the SSR molecular marker of camphor tree genome development, comprises the following steps:

[0078] 1. Camphor tree DNA extraction

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Abstract

The invention discloses a whole genome SSR molecular marker of camphor tree and its preparation method and application. The camphor tree microsatellite molecular markers developed by the present invention can be applied to the population genetic diversity detection and kinship analysis of ancient camphor trees, providing a new batch of microsatellite molecular markers for Lauraceae plants, and also for the population genetic differentiation of Lauraceae plants New tools are provided for the study of genetic diversity and structure, the level of genetic diversity of populations, the gene flow and mating system between populations, species evolution, and molecular assisted breeding.

Description

Technical field: [0001] The invention belongs to the technical field of forestry molecular biology, and specifically relates to camphor tree molecular marker technology, and more specifically relates to a camphor tree whole-genome SSR molecular marker and its preparation method and its application in the analysis of ancient camphor genetic diversity and identification of kinship. Background technique: [0002] Microsatellite (Microsatellite) is also called short tandem repeats (Short Tandem Repeats, STRs), simple sequence repeats (Simple Sequence Repeat, SSRs), simple sequence length polymorphism (SSLP). It refers to a DNA sequence that is repeated in tandem multiple times in units of 1 to 6 nucleotides. It is commonly found in the genomes of eukaryotes and prokaryotes, and even in virus genomes. SSR molecular markers have the following characteristics: repeat units and repeat numbers are highly variable, polymorphic information capacity is high; co-dominant, following Mende...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6806C12N15/11
CPCC12Q1/6806C12Q1/6895C12Q2600/156C12Q2531/113C12Q2525/151
Inventor 伍艳芳徐海宁王建汪信东郑永杰刘新亮戴小英邱凤英杨海宽
Owner JIANGXI ACAD OF FORESTRY
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