Quadruple fluorescent SSR molecular marker detection method for tea tree

Abstract

The invention discloses a quadruple fluorescent SSR molecular marker detection method for a tea tree. According to the invention, a set of detection method for quadruple SSR molecular markers in the tea tree is established by the combination of a primer combination, a touchdown PCR (polymerase chain reaction), capillary fluorescence electrophoresis and other technologies, and the method can not only improve the research efficiency and accuracy of the SSR molecule markers in the tea tree, but also save labor. However, the current detection of the SSR molecular markers in the tea tree mainly adopts a polyacrylamide gel electrophoresis technology, which has the disadvantages of low resolution, large error, low efficiency and the like; and by adopting the quadruple SSR molecular marker detection method provided by the invention, the detection of 4 pairs of SSR primers can be completed at a time. At the same time, the defects of primer annealing temperature screening, manual running rubber,artificial reading tape and the like are avoided, not only is the work efficiency greatly improved, but also the method is simple, rapid, accurate, stable and good in repeatability, and can be used for the research of tea tree variety identification, fingerprint mapping construction, population genetic structure, genetic differentiation and the like.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a tea tree quadruple fluorescent SSR molecular marker detection method. Background technique [0002] With the continuous development of molecular biology technology, especially the emergence, development and application of DNA molecular marker technology, it has greatly promoted the research on plant germplasm resources. Molecular markers refer to DNA fragments that can reflect certain differences in the genomes of organisms or populations, and directly reflect the differences between genomic DNAs. The advantages of molecular markers are as follows: (1) It is directly expressed in the form of DNA, which can be detected in various tissues and development stages of organisms, and is not restricted by seasons and environments, and there is no question of whether it is expressed or not; (2) ) are extremely numerous and spread throughout the entire genome, with almost unlimited dete...

AI Technical Summary

Problems solved by technology

At present, tea tree SSR molecular markers are mainly detected by polyacrylamide gel electrophoresis, and only one marker can be detected at a time, so the operation is complicated, the efficiency is low, and the sensitivity is poor.

At the same time, different primer annealing temperatures should be screened, which affects the efficiency of the experiment.

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