Method for predicting percentage of fructification of indica-japonica hybrid F1 by using InDel molecular markers

Abstract

The invention belongs to the technical field of molecular mark assisted breeding, and particularly discloses a method for detecting genetic differentiation level of indica-japonica hybridizing parents and predicting the percentage of fructification of hybrid F1 by using InDel molecular markers. The hybridizing parents can be purposefully selected and the hybrid F1 with high percentage of fructification is prepared by detecting the genetic differentiation level of the indica-japonica hybridizing parents. The indica-japonica genetic differentiation coefficients (GDI) of indica-japonica hybridizing parents are determined by using 40 InDel molecular markers and a built calculation method, the correlation between the indica-japonica hybridizing parents GDI and a really measured mean value of the percentage of fructification (PF) of the hybrid F1 is really analyzed, so as to form a method for analyzing parents GDI to predict the percentage of fructification of the indica-japonica hybrid F1 by the molecular markers. The indica-japonica genetic differentiation coefficient detection on the parents can be carried out before hybridization, so as to predict the percentage of fructification of the hybrid F1. Thus, the indica-japonica hybridizing parents can be effectively selected.

Description

Technical field [0001] The present invention belongs to the field of molecular marking auxiliary breeding technology. It is specifically used for the use of Indel molecular markers to detect 籼-japonica rice hybrids. 1 Strong method. technical background [0002] The use of the superbly cultivation of cultivated rice colors to cultivate 籼-japonica hybrid rice is an effective way to further improve rice production. 1 The key to yield is that the hybrid F is caused by the strong genetic differentiation of the rice-japonica rice 1 The significant decline in the strong rate affects the yield of the japonica hybrid rice, and limits the application of hybrid advantages between sub-species in hybrid rice.Therefore, before the implementation of 粳-japonica rice hybridization, effective selection can produce higher hybrid F 1 The solid -rate parent combination is the key to the advantages of hybrid between sub -species.At present, the hybrids of pupa-japonica rice in production are mainly b...

AI Technical Summary

Problems solved by technology

At present, indica-japonica hybridization in production is mainly based on the morphology and pedigree to determine the characteristics of the hybrid indica and japonica parents. In most cases, the indica-japonica hybrid F 1 The seed setting rate is low, and it is difficult to apply to production practice

The main reason is that there is no tool that can quantitatively detect the genetic differentiation of indica-japonica hybrid parents, which leads to the unpredictability of hybrid F 1 Therefore, it is urgent to establish a method of using InDel molecular markers to predict indica-japonica hybrid F 1 seed set method

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