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Duolang sheep SNP marker and its screening method and application

A Duolang sheep and marker technology, applied in the field of Duolang sheep SNP markers and its screening, can solve the problems of high cost, reducing the accuracy of sheep breed identification, restricting promotion and application, etc.

Active Publication Date: 2020-03-24
新疆畜牧科学院生物技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the interference of unrelated sites in the SNP chip, the accuracy of its identification of sheep breeds is reduced. At the same time, the high cost of chip detection and analysis also greatly restricts its large-scale promotion in the identification of genetic germplasm of sheep and its livestock products. and application

Method used

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  • Duolang sheep SNP marker and its screening method and application
  • Duolang sheep SNP marker and its screening method and application
  • Duolang sheep SNP marker and its screening method and application

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Experimental program
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Effect test

Embodiment 1

[0036] This embodiment provides a method for screening the SNP markers of Duolang sheep in Altay sheep and Bayinbulak sheep, including the following steps:

[0037] (1) Select 108 individual sheep samples including Altay sheep, Bayinbulak sheep and Duolang sheep, collect the blood of these sheep, use heparin sodium anticoagulant, use Tiangen blood genome kit to extract genomic DNA, 0.8% agarose Gel electrophoresis detection, nucleic acid protein quantification instrument to measure its concentration and purity, dilute to 100ng / ul, save for future use.

[0038] (2) Carry out the genotype analysis of the whole genome 50K SNP chip (illumina Ovine 50KBeadchip) of the prepared DNA sample, and carry out quality control to the obtained 108 individuals and 54 241 SNPs with Plink software: the individual detection rate (call rate)>0.9, SNP detection rate>0.9, minimum allele frequency (MAF)>0.01, Harberger equilibrium (HWE)>0.000001. Since the individual detection rate of one of the sh...

Embodiment 2

[0065] This embodiment provides a method for screening SNP markers of Duolang sheep in German Meat Merino and Chinese Merino sheep, comprising the following steps:

[0066] (1) Select 159 individual sheep samples including German Meat Merino, Chinese Merino and Duolang sheep, collect blood from these sheep, anticoagulate with heparin sodium, and extract genomic DNA with Tiangen Blood Genome Kit , 0.8% agarose gel electrophoresis detection, nucleic acid protein quantitative instrument to measure its concentration and purity, diluted to 100ng / ul, and stored for later use.

[0067] (2) Carry out the genotype analysis of the whole genome 50K SNP chip (illumina Ovine 50KBeadchip) of the prepared DNA sample, and carry out quality control to the 54 241 SNPs of 159 individuals obtained with Plink software: the individual detection rate (call rate)>0.9, SNP detection rate>0.9, minimum allele frequency (MAF)>0.01, Harberger equilibrium (HWE)>0.000001. After quality control, 60 German M...

Embodiment 3

[0090] See Table 14 for the comparison between the identification method of the present invention and the existing identification methods for Duolang sheep.

[0091] Table 14 Comparison between the identification method of the present invention and the existing identification method for Duolang sheep

[0092]

[0093] The SNP marker of the Duolang sheep described in the present invention can be applied to the identification of the Duolang sheep breed or its livestock products. The defect of phenotypic identification is simple and easy, and there is no interference between sites, which greatly improves the accuracy of identification of Duolang sheep breeds or their livestock products and effectively reduces costs.

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Abstract

The invention discloses a Duolang sheep SNP (single-nucleotide polymorphism) marker, and a screening method and application thereof, belonging to the field of animal molecular markers. A genetic differentiation coefficient method is utilized to perform screening on a commercialized sheep genome SNP chip and further remove high-linkage-disequilibrium SNP markers (r<2>>0.2) through linkage disequilibrium analysis, thereby obtaining the Duolang sheep SNP marker. The Duolang sheep SNP marker can be applied to identification of Duolang sheep species or animal products thereof on the premise of no obvious linkage disequilibrium among markers; and therefore, when the Duolang sheep SNP marker disclosed by the invention is used for identifying the Duolang sheep species or animal products thereof, no interference among sites exists, thereby greatly enhancing the Duolang sheep species identification accuracy and effectively lowering the identification cost.

Description

technical field [0001] The invention belongs to the field of animal molecular markers, and in particular relates to the SNP marker of Duolang sheep and its screening method and application. Background technique [0002] At present, the breed identification of sheep mainly depends on the determination and analysis of phenotypic traits. With the promotion of hybrid breeding, on the one hand, the individual phenotypes of high-generation hybrid populations are highly similar to those of their parents, and cannot be distinguished by phenotypic traits; Cannot be identified by phenotypic traits. Therefore, the pure use of phenotypic traits to identify variety resources is not accurate, comprehensive and scientific. [0003] The advent of DNA molecular genetic markers provides an effective solution to overcome this problem. DNA molecular genetic markers are often used for this work because they are not limited by phenotype, limited by time and space, and have good polymorphism. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 李文蓉贺三刚刘明军陈磊韩冰玛依拉蒋晓梅
Owner 新疆畜牧科学院生物技术研究所
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