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Duolang sheep SNP (single-nucleotide polymorphism) marker, and screening method and application thereof

A screening method, the technology of Duolang sheep, applied in the field of SNP markers and screening of Duolang sheep, can solve the problems of restricting promotion and application, high cost, reducing the accuracy of sheep breed identification, etc.

Active Publication Date: 2016-10-12
新疆畜牧科学院生物技术研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the interference of unrelated sites in the SNP chip, the accuracy of its identification of sheep breeds is reduced. At the same time, the high cost of chip detection and analysis also greatly restricts its large-scale promotion in the identification of genetic germplasm of sheep and its livestock products. and application

Method used

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  • Duolang sheep SNP (single-nucleotide polymorphism) marker, and screening method and application thereof
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  • Duolang sheep SNP (single-nucleotide polymorphism) marker, and screening method and application thereof

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Effect test

Embodiment 1

[0036] This embodiment provides a method for screening Duolang sheep SNP markers in Altay sheep and Bayinbulak sheep, including the following steps:

[0037] (1) Select 108 sheep sample individuals including Altay sheep, Bayinbulak sheep and Duolang sheep, collect blood from these sheep, use heparin sodium for anticoagulation, and use Tiangen blood genome kit to extract genomic DNA, 0.8% agarose Gel electrophoresis detection, nucleic acid protein quantifier to determine its concentration and purity, dilute to 100ng / ul, and save for later use.

[0038] (2) Perform the genotype analysis of the prepared DNA samples on the sheep whole genome 50K SNP chip (Illumina Ovine 50KBeadchip), and perform quality control on the 108 individuals and 54 241 SNPs obtained with Plink software: individual detection rate (call rate)> 0.9, SNP detection rate> 0.9, minimum allele frequency (MAF)> 0.01, Harberger balance (HWE)> 0.000001. Since the detection rate of one sheep is less than 0.9, after quali...

Embodiment 2

[0065] This embodiment provides a method for screening Duolang sheep SNP markers in German meat merino and Chinese merino sheep, including the following steps:

[0066] (1) Select 159 individual sheep samples containing German meat merino, Chinese merino sheep and Duolang sheep, collect the blood of these sheep, use heparin sodium for anticoagulation, and use Tiangen blood genome kit to extract genomic DNA , 0.8% agarose gel electrophoresis detection, nucleic acid protein quantifier to determine its concentration and purity, dilute to 100ng / ul, save for later use.

[0067] (2) Perform genotype analysis of the prepared DNA samples on the sheep whole genome 50K SNP chip (Illumina Ovine 50KBeadchip), and perform quality control on 54 241 SNPs of 159 individuals with Plink software: individual detection rate (call rate)> 0.9, SNP detection rate> 0.9, minimum allele frequency (MAF)> 0.01, Harberger balance (HWE)> 0.000001. After quality control, 60 German meat merino (GMM), 60 Chinese ...

Embodiment 3

[0090] The comparison between the identification method of the present invention and the existing method for identifying Duolang sheep is shown in Table 14.

[0091] Table 14 Comparison of the identification method of the present invention and the existing method of identifying Duolang sheep

[0092]

[0093] The Duolang sheep SNP marker of the present invention can be applied to the identification of Duolang sheep breeds or their livestock products. When the Duolang sheep SNP marker of the present invention is used to identify Duolang sheep breeds or their livestock products, it overcomes The defects of phenotypic identification are simple and easy to implement, and there is no interference between sites, which greatly improves the accuracy of the identification of Duolang sheep breeds or their livestock products and effectively reduces costs.

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Abstract

The invention discloses a Duolang sheep SNP (single-nucleotide polymorphism) marker, and a screening method and application thereof, belonging to the field of animal molecular markers. A genetic differentiation coefficient method is utilized to perform screening on a commercialized sheep genome SNP chip and further remove high-linkage-disequilibrium SNP markers (r<2>>0.2) through linkage disequilibrium analysis, thereby obtaining the Duolang sheep SNP marker. The Duolang sheep SNP marker can be applied to identification of Duolang sheep species or animal products thereof on the premise of no obvious linkage disequilibrium among markers; and therefore, when the Duolang sheep SNP marker disclosed by the invention is used for identifying the Duolang sheep species or animal products thereof, no interference among sites exists, thereby greatly enhancing the Duolang sheep species identification accuracy and effectively lowering the identification cost.

Description

Technical field [0001] The invention belongs to the field of animal molecular markers, and specifically relates to Duolang sheep SNP markers and screening methods and applications thereof. Background technique [0002] At present, the identification of sheep breeds mainly depends on the determination and analysis of phenotypic traits. With the promotion of cross breeding, on the one hand, the individual phenotypes of the high-generation hybrid populations are highly similar to those of their parents, which cannot be distinguished by phenotypic traits; on the other hand, animal products can no longer show the phenotypic characteristics of the species, and Unable to use phenotypic traits for identification. Therefore, it is not enough to accurately, comprehensively and scientifically use phenotypic traits to identify variety resources. [0003] The advent of DNA molecular genetic markers provides an effective way to overcome this problem. DNA molecular genetic markers are often us...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 李文蓉贺三刚刘明军陈磊韩冰玛依拉蒋晓梅
Owner 新疆畜牧科学院生物技术研究所
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