Peony genome DNA molecule marking method

A technology of DNA molecules and labeling methods, applied in the direction of recombinant DNA technology, DNA/RNA fragments, biochemical equipment and methods, etc., can solve the problems of low efficiency, achieve broad application prospects, good effects, and reduce costs

Inactive Publication Date: 2014-05-28
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If you want to carry out this type of marking, you must first obtain the LTR sequence of the retrotransposon, and the efficiency of the current method for isolating the LTR sequence is still low

Method used

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  • Peony genome DNA molecule marking method
  • Peony genome DNA molecule marking method
  • Peony genome DNA molecule marking method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A kind of peony genomic DNA molecular marker method is characterized in that: its specific operation steps are:

[0051] Step 1. Extraction of Peony Genomic DNA: Peony Genomic DNA was extracted using the improved CTAB method, and a total of 40 peony varieties were extracted (including Gejinzi, Wanhuasheng, Daomin, Luhefen, Huhong, Baiyu, Three Variations of Saiyu, Zhongshenghong, Green Fragrant Ball, Quhaohao, Erqiao, Oolong Pengsheng, Rouge Figure, Red Crown and Jade Belt, Meat Lotus, Silver and Red Pair, Peach Red, Jindao, Blue Sea and Bibo, Yu Jiyan Clothing, Shouxing Red, Xiaohu Hong, Silver Scale Jade Pearl, Red Lantern, Shengdan Furnace, Yao Huang, Pingshiyan, Arhat Red, Brocade Red, Red Lotus, Buddhist Cassock, Bingshan Jadeite, First Case Red, Number One Scholar Red , Rose Purple, Daojin, Dahuahuang, Purple Peony, Sichuan Peony, Yangshan Peony) genomic DNA;

[0052] Step 2. Digestion of Peony Genomic DNA: Use the Mse I and EcoR I restriction endonucleases in th...

Embodiment 2

[0058] A kind of peony genomic DNA molecular marker method is characterized in that: its specific operation steps are:

[0059] Step 1. Extraction of Peony Genomic DNA: Peony Genomic DNA was extracted using the improved CTAB method, and a total of 40 peony varieties were extracted (including Gejinzi, Wanhuasheng, Daomin, Luhefen, Huhong, Baiyu, Three Variations of Saiyu, Zhongshenghong, Green Fragrant Ball, Quhaohao, Erqiao, Oolong Pengsheng, Rouge Figure, Red Crown and Jade Belt, Meat Lotus, Silver and Red Pair, Peach Red, Jindao, Blue Sea and Bibo, Yu Jiyan Clothing, Shouxing Red, Xiaohu Hong, Silver Scale Jade Pearl, Red Lantern, Shengdan Furnace, Yao Huang, Pingshiyan, Arhat Red, Brocade Red, Red Lotus, Buddhist Cassock, Bingshan Jadeite, First Case Red, Number One Scholar Red , Rose Purple, Daojin, Dahuahuang, Purple Peony, Sichuan Peony, Yangshan Peony) genomic DNA;

[0060] Step 2. Digestion of Peony Genomic DNA: Use the Mse I and EcoR I restriction endonucleases in th...

Embodiment 3

[0066] A kind of peony genomic DNA molecular marker method is characterized in that: its specific operation steps are:

[0067] Step 1. The genomic DNA of peony was obtained by using the improved CTAB method to extract a total of 40 peony varieties (including Gejinzi, Wanhuasheng, Daomin, Luhefen, Huhong, Baiyu, Sanbian Saiyu, Zhong Raw Red, Green Fragrance Ball, Que Hao, Er Qiao, Oolong Holding Sheng, Rouge Figure, Red Crown and Jade Belt, Meat Hibiscus, Silver Red Delicate Pair, Peach Red Flattery, Golden Island, Blue Sea and Bibo, Concubine Concubine Dress, Longevity Red, Small Hu Hong, Silver Scale and Jade Pearl, Red Lantern, Sheng Danlu, Yao Huang, Ping Shiyan, Arhat Red, Brocade Pao Red, Red Lotus, Buddhist Cassock, Bingshan Emerald, First Case Red, Number One Scholar Red, Rose Purple, Island Brocade, Genomic DNA of Dahuahuang, Purple Peony, Sichuan Peony, Yangshan Peony);

[0068] Step 2. Digestion of Peony Genomic DNA: Use the Mse I and EcoR I restriction endonucleas...

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Abstract

A peony genome DNA molecule marking method is as follows: extracting a peony genomic DNA for enzyme digestion, connecting an enzyme digestion product with a joint DNA; performing PCR amplification of a connection product and a pre amplified primer to obtain a pre amplified product; taking the pre amplified product for PCR amplification with iPBS primer which is selectively amplified by use of an incision enzyme joint to obtain a PCR selectively-amplified product; then taking the PCR selectively-amplified product to add into a dyeing liquid, applying a sample for electrophoresis, after the electrophoresis, silver staining, observing, photographing and analyzing results. The peony genome DNA molecule marking method is a molecule marking technology based on the PCR and enzyme digestion, and has the advantages of simple operation, low cost and strong applicability; designed for the peony genome study technology, a new molecule marking system based on the retrotransposon is developed, and provides a new technological means for researches on genetic differentiation and evolution of germplasm resources of peony.

Description

technical field [0001] The invention relates to a molecular marker method, in particular to a molecular marker method of peony genome DNA. Background technique [0002] peony( Paeonia Suffruticosa Andr.) is Paeoniaceae (Paeoniaceae) genus Paeoniae ( Paeonia L.) Peony group (Sect. Mouton DC.) Deciduous shrub is an important ornamental greening plant and resource plant. Its flowers are beautiful in shape, bright in color and fragrant, and are praised by people of all ages. history. In the long-term cultivation and domestication process of Chinese peony, more than 1,000 cultivars with different petal colors, numbers, flower shapes and leaf shapes have been formed, represented by the Central Plains, Northwest, Jiangnan and Southwest varieties groups, and the cultivation areas are all over the country. everywhere. There are 9 species in the peony group (Hong Deyuan and Pan Kaiyu, 1999; Hong and Pan, 2007), distributed in 9 provinces including Henan, Gansu, Shaanxi, Shan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6855C12Q1/686C12Q2525/191C12Q2531/113
Inventor 郭大龙侯小改段亚宾魏冬峰吕静霞
Owner HENAN UNIV OF SCI & TECH
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