Method for detecting single nucleotide polymorphism of genes related to yean trait of milk goat

Abstract

The invention relates to the technical fields of high-throughput sequencing, genomics and bioinformatics, in particular to a detection method for analyzing single nucleotide polymorphism of genes related to yean trait of a milk goat and candidate genes by adopting a selective elimination analysis method on the basis of the single nucleotide polymorphism of the milk goat. The method comprises the following steps of 1) comparing data passing through the high-throughput sequencing to a reference genome, then annotating SNP (single nucleotide polymorphism) sites of the milk goat by utilization of free ANNOVAR software; 2) evaluating a sequencing sequence of two groups with different yean properties by utilization of a genetic differentiation coefficient (Fst) and heterozygosity scores (Hp) so as to screen out SNPs of each sliding window; 3) screening out homozygous SNPs from a low-yield group and a high-yield group, and authenticating single-base nonsense substitution and nonsynonymous substitution specifically existed in the two groups. The method disclosed by the invention has the advantages that the structure and function of protein may be affected due to the fact that the translation and amino acid sequences of the protein are directly affected through two variations, namely the single-base nonsense substitution and the nonsynonymous substitution.

Description

Technical field: [0001] The invention is mainly applied in the field of molecular genetic assisted breeding, relates to the fields of high-throughput sequencing, genomics and bioinformatics technology, and specifically relates to SNPs molecular markers and applications related to lambing traits of dairy goats. Background technique: [0002] Dairy goats occupy an important position in my country's animal husbandry production. It can provide fur, meat and dairy products, etc., and its breeding and expansion of improved breeds is one of the important issues in the development of animal husbandry industry. my country's dairy goat industry is in a process of rapid development in recent years. However, the research on the genetic background of excellent dairy goat breeds with high fecundity and high milk yield is still in its infancy. The unique genetic information contained in quantitative traits will become the basis of genetic marker-assisted breeding. [0003] The lambing trai...

AI Technical Summary

Problems solved by technology

The subpopulations of traits with quantitative differences that can be distinguished through natural or artificial selection, if they contain SNPs associated with the traits, genetic statistics can be carried out through the statistical method of selective elimination analysis, because the SNPs associated with the target traits are often They appear in clusters. If chromosome crossover or individual new mutations near this site are not considered, the group of SNPs may be enriched in an adjacent region of the target population, resulting in a decrease in heterozygosity in this region.

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