5 results about "Genetic library" patented technology

This invention relates to the field of biomedical technology, specifically to a snake-derived nanobody against KLH and its preparation and application. The invention involves immunizing the black-browed rat snake with KLH, extracting total RNA from its splenic lymphocytes, constructing a phage display VH gene library, and, after multiple rounds of screening, obtaining nanobody sequences that specifically bind to KLH, such as KLH-1 and KLH-2. This nanobody can be efficiently and solublely expressed and purified in *E. coli*, exhibiting characteristics of small molecular weight and high affinity. This invention further provides applications for this nanobody, particularly in the preparation of KLH-targeting detection reagents or drug delivery systems. This nanobody provides a core tool molecule for developing highly sensitive KLH-targeting detection reagents, efficient drug delivery systems, and novel vaccines.

This invention discloses a Valenciane oxidase mutant and its application in the preparation of naringin. The invention constructs a gene library of the HPO mutant using site-directed mutagenesis. In vitro catalytic experiments using resting cells show that, under the same conditions, the optimal HPO mutant (HPO_G302G-V480A-V482A-A484A, whose amino acid sequence is shown in SEQ ID No. 4 and nucleotide sequence in SEQ ID No. 5) exhibits a catalytic efficiency 2.54 times that of the wild type, thereby increasing substrate utilization efficiency and improving the yield of the target compound, naringin. Furthermore, the bio-preparation method for naringin described in this invention is simple to operate, environmentally friendly, and uses mild reaction conditions, showing great application potential. In addition, this invention is the first report of successfully constructing a beneficial HPO mutant for high-yield naringin production.

The application belongs to the technical field of gene detection, and particularly relates to a construction method and kit of a gene library for detecting new mutations of dominant monogenic diseases before embryo implantation. The application provides a construction method of a gene library for detecting new mutations of dominant monogenic diseases before embryo implantation, which comprises the step of amplifying the gene of the new mutation of dominant monogenic diseases before embryo implantation and related copy number variation fragments in the genomic DNA of specific screening. The application precisely applies NGS sequencing technology to the screening of dominant monogenic diseases in trace cell samples, breaks through the technical bottleneck of high-throughput sequencing under extremely low initial DNA amount in the past, and realizes the screening of new mutations before embryo implantation, thereby providing a brand-new key technical path for blocking genetic birth defects at an earlier stage.

A gene library construction method, the method comprising: providing a first gene fragment; providing a template gene fragment; combining the first gene fragment with the template gene fragment to obtain a target gene sequence having a sequence length greater than that of the first gene fragment; and performing PCR amplification on the target gene sequence to obtain a gene library.