Gene sequencing chip and gene sequencing method

Abstract

The invention provides a gene sequencing chip. The gene sequencing chip comprises an upper substrate and a lower substrate, wherein the upper substrate comprises a plurality of liquid inlets used for liquid drops to enter; the lower substrate is opposite to the upper substrate and separated from the upper substrate by a gap; the gap is used for storing the liquid drop; the lower substrate comprises a liquid drop operation area; the liquid drop operation area comprises a plurality of first line arrays used for preparing a gene library, and a plurality of second line arrays used for gene sequencing; each of the plurality of first line arrays is adjacent to each of the plurality of first line arrays. The gene sequencing chip and the gene sequencing method can accurately control minute drop to perform liquid drop movement, fusion and separation operation through a digital microfluidic technology, and programmatically accomplish all steps of the entire gene sequencing from library preparation up to gene detection on one chip. The chip is high in integration level, can accurately control the liquid drop, reduces the consumption of reagents, and is simple and mature in preparation technology.

Description

technical field [0001] The invention relates to the technical field of gene sequencing, in particular to a gene sequencing chip and a gene sequencing method. Background technique [0002] The existing gene sequencing chip products are all performed on separate chips for library preparation and gene sequencing: first, the preparation of the gene library is completed by manual operation or on a digital microfluidic chip, and then the prepared library is injected into the sequencing chip. The sequencing step is performed in a chip. This traditional step technology is relatively mature. For example, Illumina uses a digital microfluidic chip for library preparation, and then uses another sequencing chip for the gene sequencing step. In this traditional sequencing method, the gene library needs to be prepared separately, and then the obtained library is manually added to the sequencing chip. The operation steps involve a lot of manual operations, which is not conducive to shorten...

AI Technical Summary

Problems solved by technology

In this traditional sequencing method, the gene library needs to be prepared separately, and then the obtained library is manually added to the sequencing chip. The operation steps involve more manual operations, which is not conducive to shortening the sequencing cycle. A large library dose is required to meet the loss of manual transfer liquid, which increases the consumption of various reagents, increases the cost of reagents and time, and is not conducive to the improvement of automation and cost reduction; in addition, the chips used in traditional sequencing are usually Based on electroosmotic flow drive or pressure drive, larger flow channels and more reagent volume are required, and precise manipulation of droplets cannot be performed, which also increases the cost of sequencing-related reagents

Due to the different technical principles of library preparation and gene sequencing in the traditional sequencing process, the entire process cannot be realized on the same chip. Metered use, resulting in cost that cannot be reduced

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