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A method for predicting the seed-setting rate of indica-japonica rice hybrid f1 using indel molecular markers

A molecular marker and seed setting rate technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, applications, etc., can solve the problems of low seed setting rate, difficult to apply to production practice, unpredictable hybrid seed setting rate level, etc.

Inactive Publication Date: 2015-07-29
FUDAN UNIV
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Problems solved by technology

At present, indica-japonica hybridization in production is mainly based on the morphology and pedigree to determine the characteristics of the hybrid indica and japonica parents. In most cases, the indica-japonica hybrid F 1 The seed setting rate is low, and it is difficult to apply to production practice
The main reason is that there is no tool that can quantitatively detect the genetic differentiation of indica-japonica hybrid parents, which leads to the unpredictability of hybrid F 1 Therefore, it is urgent to establish a method of using InDel molecular markers to predict indica-japonica hybrid F 1 seed set method

Method used

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  • A method for predicting the seed-setting rate of indica-japonica rice hybrid f1 using indel molecular markers

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Embodiment 1

[0026] Utilize the method of the present invention to detect the genetic differentiation level of indica-japonica hybrid parents and use it to predict hybrid F 1 seed setting rate.

[0027] 1. Select a total of 120 types including typical indica rice (20), indica rice (30), typical japonica rice (20), japonica rice (30) and indica-japonica differentiation intermediate type (20), and the seed setting rate is greater than 90%. The experimental groups were constructed from several rice varieties, and the DNA of all rice varieties included in the experimental population was obtained by using the conventional genomic DNA extraction method, and the above-mentioned DNA was amplified by PCR with 40 InDel molecular markers, and the amplified products were electrophoresed on agarose gel or Polyacrylamide gel electrophoresis and other methods were used to separate the genotype data of all rice varieties at 40 InDel sites. The indica-japonica genetic differentiation coefficient ( GDI )....

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Abstract

The invention belongs to the technical field of molecular mark assisted breeding, and particularly discloses a method for detecting genetic differentiation level of indica-japonica hybridizing parents and predicting the percentage of fructification of hybrid F1 by using InDel molecular markers. The hybridizing parents can be purposefully selected and the hybrid F1 with high percentage of fructification is prepared by detecting the genetic differentiation level of the indica-japonica hybridizing parents. The indica-japonica genetic differentiation coefficients (GDI) of indica-japonica hybridizing parents are determined by using 40 InDel molecular markers and a built calculation method, the correlation between the indica-japonica hybridizing parents GDI and a really measured mean value of the percentage of fructification (PF) of the hybrid F1 is really analyzed, so as to form a method for analyzing parents GDI to predict the percentage of fructification of the indica-japonica hybrid F1 by the molecular markers. The indica-japonica genetic differentiation coefficient detection on the parents can be carried out before hybridization, so as to predict the percentage of fructification of the hybrid F1. Thus, the indica-japonica hybridizing parents can be effectively selected.

Description

technical field [0001] The invention belongs to the technical field of molecular marker-assisted breeding, and is specifically a method of using InDel molecular markers to detect the genetic differentiation level of indica-japonica hybrid parents and using it to predict hybrid F 1 Method of fruiting rate. technical background [0002] Breeding indica-japonica hybrid rice by using super heterosis among cultivated rice subspecies is an effective way to further increase rice yield, and the hybrid seed setting rate is the key factor determining the indica-japonica hybrid F 1 The key to yield, due to the strong genetic differentiation of indica-japonica rice, its hybrid F 1 The seed setting rate decreased significantly, which affected the yield of indica-japonica hybrid rice and limited the application of inter-subspecies heterosis in hybrid rice. Therefore, efficient selection prior to the implementation of indica-japonica hybrids can produce hybrids with higher F 1 The paren...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68A01H1/02A01G16/00A01G22/22
Inventor 卢宝荣刘苹蔡星星
Owner FUDAN UNIV
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