Self-polymerization polypeptide and preparation method and application thereof

A technology for synthesizing peptides and amino acids, applied in the field of biotechnology peptides and medical materials, can solve the problems of complex drug components, toxicity, poor biocompatibility, etc., and achieve the effect of broad application prospects.

Inactive Publication Date: 2009-08-26
西安蓝晶生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Surgical instruments, such as cauterizers, forceps, and clips, must be used by professionals under controlled conditions. Many of the above-mentioned drugs have complex ingredients, or contain refractory polymer materials, have poor biocompatibility, and are toxic, etc.

Method used

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  • Self-polymerization polypeptide and preparation method and application thereof
  • Self-polymerization polypeptide and preparation method and application thereof
  • Self-polymerization polypeptide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
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Embodiment

[0044] X0 is Val, X1 is Arg, X2 is Val, X3 is Asp, X4 is Val, and when the sequence is repeated twice (n=2), the sequence is as follows:

[0045] Ac-Val-Arg-Val-Asp-Val-Arg-Val-Asp-Val-NH 2

[0046] Self-assembled peptide synthesis: Weigh 10 g of Fmoc-Rink-MBHA-resin resin (degree of substitution d=0.87), use 200 ml of 20% hexahydropyridine / dimethylformamide solution to remove the FMOC protecting group, and use a peptide synthesizer Or manual polypeptide synthesis method, step-by-step coupling of the sequence polypeptide described in the general formula from the N-terminal to the C-terminal, linking amino acids, Fmoc-amino acids, catalysts (HBTU, HOBT) to resin molar ratio of 2 times the amount, using DIEA to do Catalysis, activated in DMF for 5 minutes, added to the polypeptide reactor for amino acid coupling, Kaise test whether each step of connection is complete, monitor, repeat the above steps until the sequence synthesis is complete, after the last amino acid Fmoc- depro...

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Abstract

The invention discloses a synthetic polypeptide, the amino acid sequence of the synthetic polypeptide meets the following general formula: Ac-X0-(X1-X2-X3-X4)n-NH2, wherein Ac is an acetylized N-terminal; X0 is an L-amino acid, and the type of the amino acid is Ala, Val or Gly; X1, X2, X3 and X4 are all L-amino acids or D-amino acids, X1 is a positive charge amino acid Arg, Lys or His, X3 is a negative charge amino acid Asp or Glu, and X2 and X4 are amino acids Ala, Val or Gly without charge; X1-X2-X3-X4 is a repeated sequence; and n is repeated times of amino acid arrangement in the sequence, and is equal to 2, 3 and 4. The polypeptide has the characteristics of forming a nano fiber screen through self-assembly, and can form a gel-like fiber screen in the particle environment in vitro or in vivo for hemostasis or wound nursing.

Description

technical field [0001] The invention relates to the application of a self-polymer polypeptide and the polypeptide gel fiber formed therein in hemostasis and wound healing promotion, and belongs to the field of biotechnology polypeptide and medical materials. Background technique [0002] Any surgical operation without exception involves bleeding and hemostasis. Surgical operations or trauma, especially bleeding and oozing of large wounds, are sometimes difficult to control, which will not only lead to increased blood loss, but also increase the difficulty of the operation, and even endanger life. Good hemostasis is the premise and prerequisite for safe operation. Assure. Hemostatic drugs can produce hemostatic effect by constricting small arteries and capillaries, or enhancing platelet function, or accelerating and strengthening the blood coagulation process, or inhibiting the blood clot dissolution process. [0003] Hemostasis technology has developed from simple instrume...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K7/08C07K1/06C07K1/04A61K38/08A61K38/10A61P7/04A61P17/02
Inventor 王亚宏
Owner 西安蓝晶生物科技有限公司
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