Centrifugal liquid drop microfluidic chip

Abstract

The invention relates to a centrifugal liquid drop microfluidic chip. The centrifugal liquid drop microfluidic chip comprises a substrate and a cover plate arranged on the substrate, wherein the coverplate comprises an oil phase storage cavity, a water phase storage cavity, a liquid drop generating structure connected to the oil phase storage cavity and the water phase storage cavity, a liquid drop storage cavity connected to the liquid drop generating structure and provided with at least one oil storage structure; the oil storage structure has at least one opening; and the distance from theoil phase storage cavity and the water phase storage cavity to a centrifugal shaft is less than the distance from the liquid drop storage cavity to the centrifugal shaft. The centrifugal liquid drop microfluidic chip reduces the fraction of the average dispersed phase by adjustment through the oil storage structure, improves the stability of liquid drops, and ensures that the liquid drops in a centrifugal force chip do not fuse in the storage and heating process, so that the chip has a high application value in the research application fields of biology, chemistry, medical diagnosis and the like.

Description

technical field [0001] The invention relates to a microfluidic chip, in particular to a centrifugal droplet microfluidic chip. Background technique [0002] In the field of microfluidic technology, centrifugal force drive has received extensive attention, mainly because: (1) no external pump is required, which reduces the cost and improves the portability of equipment; (2) parallel detection can be realized on the centrifugal force microfluidic chip, The detection efficiency is greatly improved; (3) The density difference between oil and water can be used to achieve the purpose of densely arranging droplets, effectively improving the space utilization rate of the structure and the convenience of reading results. [0003] The centrifugal droplet microfluidic chips currently being researched, such as the centrifugal droplet microfluidic chips disclosed in Chinese patent applications with application numbers 201710855088.9 and 201610069321.6, all rely on centrifugal force to ge...

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Problems solved by technology

However, in the actual process, under the action of centrifugal force, (1) due to the difference in density between the oil and water phases, the oil and water phases in the droplet storage chamber are continuously stratified during the centrifugation process, and the oil phase between the droplets and the surfactant The loss is increasing (analogous: the dehydration process of the washing machine), and as the number of droplets increases, the deformation of the droplets accumulated in the lower layer becomes more and more serious, and the oil film between the droplets becomes thinner and thinner, evenly dispersed The phase fraction increases, which in turn makes the stability of the droplet worse; (2) The lipophilicity and porosity of the microfluidic chip material will absorb the oil phase to a certain extent; (3) due to chemical reactions or medical diagnosis (such as polymerization) Enzyme chain reaction, ring-mediated isothermal amplification reaction), droplets often need to undergo a certain degree of heating process, the increase in temperature will increase the energy of the droplet itself, and be in a more unstable state; these factors will cause The stability of the droplets in the centrifugal microfluidic chip becomes poor, and there is a high possibility that the fusion between the droplets will eventually occur, which will cause inconvenience and inaccurate statistics of the results, and eventually lead to the failure of the experiment.

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