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Liquid drop microfluidic system and method for detecting interaction between quantum dots and biomolecules

A microfluidic system and biomolecular technology, applied in material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of complex operation and lack, and achieve the effects of high detection sensitivity, improved accuracy, and simple and easy operation.

Active Publication Date: 2013-01-16
铜陵汇泽科技信息咨询有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, there is still a lack of effective methods for the rapid reaction between quantum dots and biomolecules
At present, the main method is Surface Plasmon Resonance (SPR), but this method usually needs to fix a biomolecule first, and the operation is more complicated (SapsfordK E, Pons T, Medintz I L, et al.J.Phys .Chem.C2007, 111, 11528–11538)

Method used

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  • Liquid drop microfluidic system and method for detecting interaction between quantum dots and biomolecules
  • Liquid drop microfluidic system and method for detecting interaction between quantum dots and biomolecules
  • Liquid drop microfluidic system and method for detecting interaction between quantum dots and biomolecules

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Embodiment 1

[0029] Figure 4 The fluorescence intensity of the mixed quantum dots and biomolecules H24-Cy5 is detected by the fluorescence of the microfluidic chip. All the circles in the figure are a schematic diagram of the detection window at a fixed position on the microfluidic chip. Droplets passing through this fixed position will produce different intensities. Fluorescence signal, the larger the droplet in the circle, the greater the fluorescence intensity. The sequence of H24-Cy5 is as follows:

[0030]

[0031] The experimental conditions were: the flow rates of the first syringe pump 1, the second syringe pump 2 and the third syringe pump 3 were 3 μL min -1 , 1.5μL·min -1 and 1.5μL·min -1 . Set the detection wavelength of the fluorescence spectrometer, the detection wavelength of QDs is 612nm, the detection wavelength of Cy5 is 661nm; the concentration of QDs is 20nM, and the concentration of H24-Cy5 is 80nM.

[0032] The experimental results show that, if Figure 4 As sh...

Embodiment 2

[0034] Image 6 This is the kinetic detection of quantum dots mixed with biomolecules H6-Cy5. The experimental conditions are the same as in Example 1, the difference is that the biomolecule is H6-Cy5, and the sequence is Cy5-DDDLVPRGSGP 9 G 2 h 6 . The experimental results confirmed that the combination of QDs and H6-Cy5 reaches equilibrium in about 100s, which is similar to the results of the surface plasmon resonance method (see Sapsford, K.E.; Pons, T.; Medintz, I.L.; et al.J.Phys. Chem.C 2007,111,11528-11538.), confirmed the effectiveness of this method.

[0035] Fluorescence resonance energy transfer occurs between the dye molecule Cy5 and the quantum dots. After the quantum dots interact with the biomolecules, the fluorescence intensity gradually changes with time, so that the kinetics of the reaction can be measured. It can be seen from the above two embodiments that a droplet microfluidic system and method for detecting the interaction between quantum dots and bi...

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Abstract

The invention relates to a liquid drop microfluidic system for detecting interaction between quantum dots and biomolecules. The liquid drop microfluidic system comprises a microfluidic chip, a fluorescence microscope, three injection pumps for injecting liquid drops to the microfluidic chip, a fluorescence interface, a fluorescence spectrograph and a data acquiring and analyzing system, wherein the microfluidic chip is arranged on the focal point of an objective lens; one end of the fluorescence interface is connected with a standard port of the fluorescence microscope, and the other end of the fluorescence interface is connected with the fluorescence spectrograph through an optical fiber; and a spectral signal output end of the fluorescence spectrograph is connected with a signal input end of the data acquiring and analyzing system. The invention also relates to a method for detecting the interaction between the quantum dots and the biomolecules, the biomolecules are marked by dye molecules, and fluorescence resonance energy can be transferred between the dye molecules and the quantum dots. The liquid drop microfluidic system is easy to operate and high in detection sensitivity, and rapid dynamic detection between the quantum dots and the biomolecules can be realized; and the fluorescence spectrograph can simultaneously detect a plurality of fluorescence wavelengths, errors are reduced, and detection accuracy is improved.

Description

technical field [0001] The invention relates to the fields of biological analysis and nanotechnology, in particular to a droplet microfluidic system and method for detecting the interaction between quantum dots and biomolecules. Background technique [0002] Exploring the interaction between quantum dots (QDs) and biomolecules has become one of the core researches to deeply understand the nature of life processes. In the application of quantum dot bioanalysis, kinetic parameters are also important indicators for evaluating the interaction between quantum dots and biomolecules. Therefore, it is very important to establish suitable analytical and characterization techniques. At present, the methods that can be used to study the interaction between quantum dots and biomolecules mainly include fluorescence spectroscopy, chromatography and electrophoresis (Ostergaard J, Heegaard N H H, Electrophoresis 2003, 24, 2903-2913). However, there is still a lack of effective methods for ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 王建浩邱琳蒋鹏举王车礼夏江
Owner 铜陵汇泽科技信息咨询有限公司
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