Liquid drop microfluidic system and method for detecting interaction between quantum dots and biomolecules

Abstract

The invention relates to a liquid drop microfluidic system for detecting interaction between quantum dots and biomolecules. The liquid drop microfluidic system comprises a microfluidic chip, a fluorescence microscope, three injection pumps for injecting liquid drops to the microfluidic chip, a fluorescence interface, a fluorescence spectrograph and a data acquiring and analyzing system, wherein the microfluidic chip is arranged on the focal point of an objective lens; one end of the fluorescence interface is connected with a standard port of the fluorescence microscope, and the other end of the fluorescence interface is connected with the fluorescence spectrograph through an optical fiber; and a spectral signal output end of the fluorescence spectrograph is connected with a signal input end of the data acquiring and analyzing system. The invention also relates to a method for detecting the interaction between the quantum dots and the biomolecules, the biomolecules are marked by dye molecules, and fluorescence resonance energy can be transferred between the dye molecules and the quantum dots. The liquid drop microfluidic system is easy to operate and high in detection sensitivity, and rapid dynamic detection between the quantum dots and the biomolecules can be realized; and the fluorescence spectrograph can simultaneously detect a plurality of fluorescence wavelengths, errors are reduced, and detection accuracy is improved.

Description

technical field

[0001] The invention relates to the fields of biological analysis and nanotechnology, in particular to a droplet microfluidic system and method for detecting the interaction between quantum dots and biomolecules. Background technique

[0002] Exploring the interaction between quantum dots (QDs) and biomolecules has become one of the core researches to deeply understand the nature of life processes. In the application of quantum dot bioanalysis, kinetic parameters are also important indicators for evaluating the interaction between quantum dots and biomolecules. Therefore, it is very important to establish suitable analytical and characterization techniques. At present, the methods that can be used to study the interaction between quantum dots and biomolecules mainly include fluorescence spectroscopy, chromatography and electrophoresis (Ostergaard J, Heegaard N H H, Electrophoresis 2003, 24, 2903-2913). However, there is still a lack of effective methods for ...

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