Droplet microfluidic device and methods of sensing the results of an assay therein

A measurement method and a microfluidic technology, which are applied in fluid controllers, chemical instruments and methods, measuring devices, etc., can solve the problems of undisclosed TFT backplane circuit embodiments and other problems, and achieve the effect of low cost

Active Publication Date: 2018-04-17
SHARP LIFE SCI EU LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0016] The disadvantage of US7163612 is that it does not disclose any circuit embodiment of TFT backplane for realizing AM-EWOD

Method used

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  • Droplet microfluidic device and methods of sensing the results of an assay therein
  • Droplet microfluidic device and methods of sensing the results of an assay therein
  • Droplet microfluidic device and methods of sensing the results of an assay therein

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Embodiment Construction

[0064] In the drawings, like reference numerals indicate like components or features.

[0065] figure 2 An exemplary assay measurement system according to a first embodiment of the present invention is shown. The measurement system includes two components, such as a reader 40 and a cartridge 44 . Cassette 44 may contain a microfluidic device (eg, AM-EWOD device 41 ) as well as (not shown) fluidic input ports and electrical connections into the device. The fluid input port may perform the function of inputting fluid into the AM-EWOD device 41 and dispensing from an input reservoir, for example by electrowetting control, to generate droplets 4 within the device. 15. As further detailed below Yes, the microfluidic device includes an electrode array configured to receive an input fluid droplet.

[0066] The assay measurement system may further include a controller configured to control an actuation voltage applied to the electrode array of the microfluidic device to perform a m...

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Abstract

A method of determining the result of an assay in a microfluidic device includes the steps of: dispensing a sample droplet onto a first portion of an electrode array of the microfluidic device; dispensing a reagent droplet onto a second portion of the electrode array of the microfluidic device; controlling actuation voltages applied to the electrode array to mix the sample droplet and the reagentdroplet into a product droplet; sensing a dynamic property of the product droplet; and determining an assay of the sample droplet based on the sensed dynamic property. The dynamic property is a physical property of the product droplet that influences a transport property of the product droplet on the electrode array. Example dynamic properties of the product droplet include the moveable state, split-able state, and viscosity based on droplet properties. The method may be used to perform an amoebocyte lysate (LAL) assay.

Description

technical field [0001] The present invention relates to droplet microfluidic devices, and in particular to electrowetting on dielectric (EWOD) devices, and more particularly to active matrix electrowetting on dielectric (AM-EWOD), and to sensing in A method of determining the dynamic properties of one or more droplets on such a device as a result of chemical or biochemical testing. Background technique [0002] Electrowetting on dielectric (EWOD) is a well known technique for manipulating droplets of a fluid by applying an electric field. Active-matrix EWOD (AM-EWOD) refers to realizing EWOD in an active-matrix array incorporating transistors, for example by using thin film transistors (TFTs). Therefore, it is a candidate technology for digital microfluidics for lab-on-a-chip technology. An introduction to the rationale for this technology can be found in: "Digital microfluidics: is a true lab-on-a-chippossible?", R.B. Fair, Microfluid Nanofluid (2007) 3:245-281. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/579
CPCB01L2300/0645G01N33/579B01L3/502792B01L2400/0427B01L2300/0816B01L2300/0887
Inventor 本杰明·詹姆斯·哈德文艾德里安·马克·西蒙·雅各布詹森·罗德里克·赫克托迈克尔·詹姆斯·布朗洛足立昌浩艾莉森·玛丽·斯金纳马克·蔡尔兹
Owner SHARP LIFE SCI EU LTD
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