Liquid drop microfluidic chip and preparation method thereof

Abstract

The present invention provides a liquid drop microfluidic chip including a chip substrate, a chip channel layer, a liquid drop storage layer, a chip adhesive layer and a chip cover plate, the chip channel layer, the liquid drop storage layer, the chip adhesive layer and the chip cover plate are sequentially pressed and sealed with the chip substrate, the liquid drop storage layer and the chip adhesive layer are respectively correspondingly provided with a liquid drop channel, the opposite two ends of the liquid drop channel are respectively provided with a liquid drop inlet and a liquid drop outlet, the chip channel layer is also provided with a liquid drop generation component and a liquid drop detection / separation component, the liquid drop generation component is in pipe connection with the liquid drop inlet of the liquid drop channel, the liquid drop detection / separation component is in pipe connection with the liquid drop outlet of the liquid drop channel. The liquid drop microfluidic chip has integrated many functions such as liquid drop generation, storage, reaction and sorting, has a high integration degree and a small area, and can achieve high flux separation experiment under high screening efficiency and automation level. The invention also provides a preparation method of the chip, and the preparation method is suitable for preparation of the chip in a large scale and simple way.

Description

technical field [0001] The invention relates to the technical field of droplet microfluidic chips, in particular to a droplet microfluidic chip and a preparation method thereof. Background technique [0002] The droplet microfluidic chip is a brand-new technology for manipulating tiny volumes of liquids that has rapidly developed in the field of microfluidic chips. Under the action of micro-channels, mutually incompatible liquids can form a series of micro-droplets, and each isolated and non-interfering droplet can be used as a micro-reactor to complete related biochemical reactions and detection. Therefore, droplet microfluidic technology has the characteristics of small droplet volume, less sample consumption, flexible manipulation (droplet generation, fusion, splitting, labeling, sorting, etc.), rapid mass transfer in droplets, and high detection / sorting frequency. , especially when applied to high-throughput screening, can greatly improve the scale, speed and cost of sc...

AI Technical Summary

Problems solved by technology

[0003] At present, most of the droplet microfluidic chips used for high-throughput sorting have a single function, which leads to complex experimental procedures and low sorting repeatability; there are also some microfluidic chips integrating multiple functional units, but their structures are generally Complicated, difficult to process and high in cost

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