Droplet micro-fluidic chip and preparation method of micro-droplets

A microfluidic chip and droplet technology, applied in the field of microfluidics, can solve the problems of unsuitable research and mass production requirements, poor chip stability and repeatability, and complex droplet preparation process, so as to improve equipment reliability. And the difficulty of subsequent equipment maintenance, reducing manufacturing costs, ensuring uniformity and stability

Pending Publication Date: 2020-11-20
SHENZHEN YHLO BIOTECH
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Problems solved by technology

[0003] However, the current chip for preparing micro-droplets is not suitable for most research and mass production ne

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  • Droplet micro-fluidic chip and preparation method of micro-droplets
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  • Droplet micro-fluidic chip and preparation method of micro-droplets

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Embodiment Construction

[0022] In order to facilitate the understanding of the present invention, the following will describe the present invention more fully and give preferred embodiments of the present invention. However, the present invention can be embodied in many different forms and is not limited to the embodiments described herein. On the contrary, these embodiments are provided to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0023] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention. The terms used herein in the description of the present invention are for the purpose of describing specific embodiments only, and are not intended to limit the present invention. As used herein, the term "and / or" includes any and all combinations of one or more of the associated listed items.

[0024] At present, the methods...

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Abstract

The invention relates to a droplet micro-fluidic chip and a preparation method of micro-droplets. The droplet micro-fluidic chip comprises at least one droplet preparation unit; the droplet preparation unit comprises a dispersed phase cavity, a quantitative cavity, a capillary nozzle and a continuous phase cavity; the droplet micro-fluidic chip is provided with a rotating center, the dispersed phase cavity is provided with a sample adding hole for adding dispersed phase liquid, and the quantitative cavity is connected with the dispersed phase cavity and is further away from the rotating centerrelative to the dispersed phase cavity; the capillary nozzle is further away from the rotating center relative to the quantitative cavity, and one end of the capillary nozzle is connected with the quantitative cavity and extends from the connecting end to the direction away from the rotating center; the continuous phase cavity is connected with the end, away from the quantitative cavity, of the capillary nozzle and is further away from the rotating center relative to the capillary nozzle, and continuous phase liquid is contained in the continuous phase cavity. According to the droplet micro-fluidic chip, centrifugal force is used as droplet preparation driving force, and stable and high-speed preparation of droplets with uniform sizes can be realized through configuration of different parameters.

Description

technical field [0001] The invention relates to the field of microfluidic technology, in particular to a droplet microfluidic chip and a method for preparing a microfluidic droplet. Background technique [0002] Microfluidics refers to a technology that manipulates fluids in micron-scale space. This technology can shrink the basic functions of laboratories such as chemistry and biology to a chip of a few square centimeters, so it is also called chip experiment. room. As an important branch of microfluidic chip research, droplet microfluidics has been developed on the basis of traditional continuous flow microfluidics systems in recent years. Droplet microfluidics has been widely used in biomedicine, for example, through Precise manipulation of micro-droplets in the reaction can reduce the consumption of reaction reagents and improve the utilization of reagents. The prepared tens of thousands or even millions of picoliter-level micro-droplets with good monodispersity can be...

Claims

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Application Information

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IPC IPC(8): B01L3/00
CPCB01L3/5027B01L3/502707B01L3/50273B01L2200/10B01L2300/0861B01L2300/0887B01L2300/12B01L2400/0409B01L3/502784B01L2200/0684B01L2300/0681B01L2300/0803
Inventor 李顺基程晓宇刘笔锋钱纯亘胡鹍辉
Owner SHENZHEN YHLO BIOTECH
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