High-throughput detection system for microbe based on droplet microfluidic chip

A microfluidic chip and detection system technology, applied in the field of high-throughput detection systems for microorganisms, can solve the problems of slow progress in the research of intermittent droplets, and achieve the effects of fast speed, simple system design, and high-efficiency screening
CN104007091AInactive Publication Date: 2014-08-27TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
7 Cites 27 Cited by

Patent Information

Authority / Receiving Office
CN ยท China
Current Assignee / Owner
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Publication Date
2014-08-27
Estimated Expiration
Not applicable ยท inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
Patent Text Reader

Abstract

The invention relates to a high-throughput detection system for microbes based on a droplet microfluidic chip. The system mainly comprises a droplet microfluidic chip system (1), a light path system (2) and a data acquisition and analysis system (3), wherein the droplet microfluidic chip system (1) embeds to-be-detected microbe to an independent single droplet micro-reaction chamber; the light path system (2) is used for carrying out laser-induced fluorescence detection signal transmission of the microbe sample in the single droplet micro-reaction chamber; and the data acquisition and analysis system (3) carries out detection and analysis on the acquired signals through a computer software. The droplet microfluidic chip can be replaced. The system is suitable for laser-induced fluorescence detection and analysis of different types of microbes, has the advantages of simple design, low processing and manufacturing cost, low detection cost, fast speed and high throughput, and realizes efficient screening of production-related target enzymes and metabolites of different types of industrial microbes including escherichia coli, corynebacterium glutamicum and saccharomyces cerevisiae.
Need to check novelty before this filing date? Find Prior Art

Description

Technical field

[0001] The invention relates to a high-throughput detection system based on a droplet microfluidic chip, in particular to a high-throughput detection system for microorganisms based on laser-induced fluorescence detection of a droplet microfluidic chip. Background technique

[0002] The microbial high-throughput screening system refers to a technical platform that can automatically, quickly and efficiently screen the functions and products of microorganisms, and is one of the bottlenecks in the development and industrialization of industrial microbial resources. Screening from nature and mutation evolution in the laboratory has always been an important way to discover and innovate industrial microbial strains, and is the basis of the industrial biotechnology industry. The microbial strains used in industry are mainly obtained through mutagenesis screening. With the rapid development of modern metabolic engineering technology and synthetic biology, rational de...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More