Droplet microfluidic technology based method for preparing chromatographic packing with uniform and controllable grain size

A technology of microfluidic technology and chromatographic packing, which is applied in the field of chromatographic packing with uniform and controllable particle size based on droplet microfluidic technology, which can solve the problems of poor monodispersity and achieve the effect of excellent monodispersity

Active Publication Date: 2014-05-28
晋江精纯科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for preparing chromatographic packing with uniform particle size and controllable pa

Method used

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  • Droplet microfluidic technology based method for preparing chromatographic packing with uniform and controllable grain size
  • Droplet microfluidic technology based method for preparing chromatographic packing with uniform and controllable grain size
  • Droplet microfluidic technology based method for preparing chromatographic packing with uniform and controllable grain size

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Experimental program
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Effect test

Embodiment 1

[0026] Add 1.8 mL of tetramethoxysilane (TMOS) and 2 mL of 3-glycidyloxypropyltrimethoxysilane (GPTMS) and 540 mg of polyethylene glycol (PEG) into 5 mL of 0.01 M acetic acid solution, in an ice-water bath (0°C) under magnetic stirring for 1 h, until completely hydrolyzed into a transparent and uniform solution. Then take 2 mL of the hydrolyzed solution in a 5 mL centrifuge tube, add 80 mg of L-lysine hydrochloride, sonicate until it is completely dissolved, and use it as the dispersed phase. 0.5 g of EM90 was dissolved in 9.5 g of mineral oil as the continuous phase. The dispersed phase and the continuous phase were introduced into the microfluidic chip by using a precision syringe pump (such as figure 1 ), where the chip depth is 50 μm, the width of horizontal channel a and vertical channel b is 200 μm, the width of horizontal channel and vertical channel at the intersection is 50 μm, and the width of droplet channel c is 120 μm. At the interface, the dispersed phase was "...

Embodiment 2

[0031] Add 1.8 mL of tetramethoxysilane (TMOS) and 2 mL of 3-glycidyloxypropyltrimethoxysilane (GPTMS) and 540 mg of polyethylene glycol (PEG) into 5 mL of 0.01 M acetic acid solution, in an ice-water bath (0°C) under magnetic stirring for 1 h, until completely hydrolyzed into a transparent and uniform solution. Then take 2 mL of the hydrolyzed solution in a 5 mL centrifuge tube, add 80 mg of L-lysine hydrochloride, sonicate until it is completely dissolved, and use it as the dispersed phase. 0.5 g of EM90 was dissolved in 9.5 g of mineral oil as the continuous phase. The dispersed phase and the continuous phase were introduced into the microfluidic chip by using a precision syringe pump (such as figure 1 ), where the chip depth is 50 μm, the width of horizontal channel a and vertical channel b is 200 μm, the width of horizontal channel and vertical channel at the intersection is 50 μm, and the width of droplet channel c is 120 μm. At the interface, the dispersed phase was "...

Embodiment 3

[0033] Add 1.8 mL of tetramethoxysilane (TMOS) and 2 mL of 3-glycidyloxypropyltrimethoxysilane (GPTMS) and 540 mg of polyethylene glycol (PEG) into 5 mL of 0.01 M acetic acid solution, in an ice-water bath (0°C) under magnetic stirring for 1 h, until completely hydrolyzed into a transparent and uniform solution. Then take 2 mL of the hydrolyzed solution in a 5 mL centrifuge tube, add 80 mg of L-lysine hydrochloride, sonicate until it is completely dissolved, and use it as the dispersed phase. 0.5 g of EM90 was dissolved in 9.5 g of mineral oil as the continuous phase. The dispersed phase and the continuous phase were introduced into the microfluidic chip by using a precision syringe pump (such as figure 1 ), where the chip depth is 20 μm, the width of horizontal channel a and vertical channel b is 100 μm, the width of horizontal channel and vertical channel at the intersection is 20 μm, and the width of droplet channel c is 80 μm. At the interface, the dispersed phase was "c...

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Abstract

The invention discloses a droplet microfluidic technology based method for preparing chromatographic packing with the uniform and controllable grain size, and relates to preparation of the chromatographic packing. Tetramethoxysilane, 3-glycidoxypropyltrimethoxysilane and polyethylene glycol are added into an acetic acid solution and hydrolyzed into a hydrolysate under an ice-water bath, and a water-soluble basic amino acid is added and subjected to ultrasound until complete dissolution to be used as a disperse phase; an oil-soluble surfactant is dissolved into oil to be used as a continuous phase; the disperse phase is introduced into a horizontal channel of a microfluidic chip, the continuous phase is introduced into a vertical channel of the microfluidic chip, and the ratio of flow velocities of the disperse phase and the continuous phase is adjusted at the interface of the horizontal channel and the vertical channel to produce droplets with different grain sizes; the droplets have a condensation reaction at the temperature of 30-50 DEG C for 10-14 h and have an epoxide ring-opening reaction at the temperature of 60-80 DEG C for 10-14 h respectively, then isopropanol and methyl alcohol aqueous solutions are used for alternative washing respectively, and a product is obtained through vacuum drying.

Description

technical field [0001] The invention relates to the preparation of chromatographic fillers, in particular to a method for preparing chromatographic fillers with uniform and controllable particle size based on droplet microfluidic technology. Background technique [0002] High performance liquid chromatography (HPLC) is an important branch of modern chromatography. HPLC is widely used in petrochemical, life science, pharmaceutical industry, environmental monitoring and other fields due to its high speed, high efficiency and high sensitivity. The chromatographic column is one of the most important components of high-performance liquid chromatography. The stationary phase in the chromatographic column, that is, the chromatographic column packing (or separation material or separation medium) is the core of chromatographic separation. High-quality chromatographic packing is the key to obtaining high-efficiency chromatography. The key to separation. High-efficiency chromatography...

Claims

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Application Information

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IPC IPC(8): B01J20/30B01J20/281B01J19/00
Inventor 张博王鑫丁芳芳王林周拙恒
Owner 晋江精纯科技有限公司
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