High-throughput screening apparatus using droplet micro-fluidic chip
A technology of microfluidic chips and screening equipment, applied in the direction of microorganism-based methods, biochemical equipment and methods, specific-purpose bioreactors/fermenters, etc. Droplet screening solutions and system integration need to be improved to achieve the effects of saving droplet samples, simple droplet supply, and low processing and manufacturing costs
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[0116] Example: Fluorescent detection of Bacillus subtilis droplets expressing red fluorescent protein
[0117] Pick a single colony from a fresh Bacillus subtilis LB solid plate expressing red fluorescent protein to LB liquid medium, culture at 37°C and 200rpm until OD600=0.6-0.8, wash the cells with PBS buffer at pH 7.4 3 times, 775g each time, 5min, and finally resuspend the bacteria with PBS buffer, dilute the bacteria to OD600=0.125, select the cell embedding chip for droplet embedding of cells, set the flow rate of the oil phase to 200 μL / h, The flow rate of the Bacillus subtilis cell diluent was 50 μL / h, and the generated droplets were injected into a 1mL syringe. The ratio of burying two or more cell droplets is 0.0902.
[0118] The droplet detection chip 2 is placed on the stage 37, and the position of the stage 37 is adjusted so that the droplet detection chip 2 is clearly imaged on the high-speed camera. Set the droplet flow rate to 10 μL / h, the oil phase flow rat...
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