High-throughput screening apparatus using droplet micro-fluidic chip

Abstract

The invention provides a high-throughput screening apparatus using a droplet micro-fluidic chip. The apparatus comprises: a rack; a micro-fluidic control system constructed to control a fluid supply device for supplying a droplet-containing fluid to the droplet micro-fluidic chip; a photoelectric detection system comprising an incident light generation device for limiting outgoing and incident light paths, a preferable laser excitation device and a fluorescence detection device for limiting a fluorescent light path; an electric screening system constructed to connect the droplet micro-fluidicchip in order to screen droplets flowing through the droplet micro-fluidic chip; and one or more controllers constructed to receive a signal from the fluorescence detection device, compare the signalwith a predetermined threshold and control the electric screening system to perform droplet screening according to the obtained comparison result.

Description

technical field [0001] The invention relates to a high-throughput screening device using a droplet microfluidic chip, especially a single-cell screening device. The invention also relates to a droplet screening method using the device. Background technique [0002] Screening from nature and mutation evolution in the laboratory has always been an important way to develop and innovate industrial microbial strains, and is the foundation of the industrial biotechnology industry. The microbial strains currently used in industry are mainly obtained through mutagenesis screening. At present, the screening of microbial strains mostly adopts micro-plate method (96-well plate or 384-well plate) or plate method. These methods almost all rely on manual operation, and it takes a long time to screen more than 100,000 mutant libraries and consumes a lot of time. The cost of consumables and reagents is unaffordable. The microbial high-throughput screening platform uses advanced modern au...

AI Technical Summary

Problems solved by technology

However, all kinds of high-throughput screening equipment that have been put into use are mainly large-scale equipment systems that are expensive and complex to operate developed by foreign companies. In particular, flow cytometers are required as the main component to meet high-throughput requirements. The flow cytometer provided by the company is expensive and requires special personnel to operate. The screened object needs to have fluorescent signals on the surface or inside, and cannot detect and screen metabolites secreted by cells, which limits its application range.

[0003] The Chinese invention patent "A System and Method for Fluorescence Detection of Droplet Samples" (authorized announcement number CN104388307A) discloses a system and method for fluorescence detection of droplet samples, but there are still the following problems: (1) lack of droplet screening unit; (2) System integration needs to be improved, which is not conducive to popularization

[0004] The British journal "Nature protocols" published in 2013 published the document "Single-cell analysis and sorting using droplet-based microfluidics", which is still in the stage of laboratory research and does not exist Integrated System Solutions

[0006] The applicant's Chinese invention patent application "A Microscope Integrated Optical System for Droplet Fluorescence Detection" (publication number CN105300943A) provides an optical path system for droplet fluorescence detection, but does not propose a highly integrated Overall Droplet Screening Solution

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