160 results about "Droplet flow" patented technology

A thermal cycling device (3) device a number of fixed thermal zones (11, 12, 13) and a fixed conduit (10) passing through the thermal zones. A controller maintains each thermal zone including its section of conduit (10) at a constant temperature. A series of droplets flows through the conduit (10) so that each droplet is thermally cycled, and a detection system detects fluorescence from droplets at all of the thermal cycles. The conduit is in a single plane, and so a number of thermal cycling devices may be arranged together to achieve parallelism. The flow conduit comprises a channel (17) and a capillary tube (10) inserted into the channel. The detection system may perform scans along a direction to detect radiation from a plurality of cycles in a pass.

Drop-to-drop connection schemes are described for putting a microfluidic device in fluidic communication with a fluid source or another microfluidic device, including but not limited to, putting a microfluidic device in fluidic communication with the perfusion manifold assembly.

The invention provides a single cell separation method based on droplet micro-fluidic chips. The method concretely comprises the following steps: A, a single cell suspension flows into a dispersed phase inlet channel from a dispersed phase inlet; B, an oil phase liquid flows into a continuous phase inlet channel from a continuous phase inlet; C, the above two phases merge to form droplets enclosing single cells, the droplets flow over a droplet capturing unit with the generation of a large number of droplets in a liquid storage pool, and stand for 2-5 min, and the superfluous droplets are sucked out when the droplets slowly settle into the droplet capture unit; and D, the droplet chips which capture the single cells are cultured in a 37 DEG C incubator, then DAPI is added to carry out nuclear staining, and the single cell capture rate is detected. The method has the advantages of simplicity and rapidity in operation, small use amounts of the cells and reagents, low experiment cost, high integration and wide application range.

A high-throughput micro-droplet immobilization method, based on the principle of buoyancy and surface tension, is controlled on a microfluidic chip. The micro-droplet immobilization method specifically includes: injecting the continuous phase and the dispersed phase into the chip using a syringe pump, As a result, continuous monodisperse microdroplets are formed in the T-shaped microdroplet generation zone; the microdroplets continue to flow and enter the droplet catcher array driven by the syringe pump, and the droplets flow through the channel below the catcher due to buoyancy and The combined effect of surface tension enters the cylindrical catcher upwards; subsequent droplets flow through the lower channel of the droplet catcher that has successfully immobilized the droplet, and are sequentially captured in subsequent droplet catchers. The invention simplifies the principle and process of liquid droplet immobilization, the microfluidic chip involved is simple to manufacture, convenient to operate in experiments, stable in immobilization effect, does not cause damage to organisms in the liquid droplet, and is easy to carry out with other functional units on the chip. Flexible combination and scale integration.

The instrument cold sprays a solution sample at a low temperature and desolvates the sample. Under this condition, a mass analysis is performed. The instrument comprises a needle pipe (8) through which the solution sample is passed, a sheath tube (24) formed coaxially with the needle pipe (8) and passing a temperature-controlled nebulizing gas therethrough, the above-described desolvation block (3), means for cooling the block (15), means for heating the block (4), and a temperature sensor (5) for detecting the temperature of the block (3). The block (3) has a passage for charged liquid droplets of the solution sample cold sprayed from the tip of the needle pipe (8), and acts to remove the solvent from the charged liquid droplets flowing through the passage. A coldspray mass spectrometer is offered which has a desolvation block whose temperature can be easily controlled. In the instrument, condensation of water and electrical leakage are prevented for a long time. The instrument can perform measurements stably and is easy to handle.

The invention discloses a production process of rubber-dipped gloves, which comprises the following steps: a. Dipping: put the gloves to be dipped on a hand mold, and immerse the palm of the gloves on the hand mold into water-based PU glue at normal temperature Middle; b, glue dropping: lift the hand model into the air, so that the excess water-based PU glue drips clean; c, uniform glue: make the hand model rotate 360; d, soak the coagulant: soak the gloves in step c to solidify e, dripping coagulant: lift the hand model into the air, so that the excess coagulant drips clean; f, washing: immerse the gloves in the sink for washing; g, drying: put the hand model and gloves in the oven Drying moisture; h, demoulding: the glove after drying is taken off from hand mold, obtains the dipped glove. There is no DMF volatilization in this process, and there is no DMF residue in the produced gloves, which avoids damage to the health of workers and is environmentally friendly and pollution-free; the gloves have excellent wear resistance and tear resistance; the gloves are soft but not sticky, dry and breathable.

The present application relates to a continuous droplet flow microfluidic system, including a microfluidic chip including an optical detection section; a stage assembly including a microfluidic chip holder configured to receive the microfluidic chip and a plurality of heating elements arranged to heat a plurality of separate sections of the microfluidic chip to a corresponding plurality of different temperatures; and an optical detection system arranged to detect fluorescent light emitted from said optical detection section of the microfluidic chip.

The invention provides a device and a method for flow cytometric fractionation of particles contained in a fluid stream, wherein sections of the fluid stream, especially droplets if the fluid stream is a droplet stream, are irradiated with a laser. The laser disposed for the irradiation of the sections of the fluid stream can have a wavelength which is absorbed by the fluid and can have a sufficient radiation duration and radiation intensity to deflect sections of the fluid stream.

The invention relates to a method for synthesizing carbon micron-spheres. The method comprises the following steps: dissolving furfuryl alcohol in a water-insoluble oil phase to prepare an oil phase solution; dissolving acid catalyst in water to prepare a water phase solution; delivering the oil phase solution and the water phase solution into a micro-reactor from the two inlets of the microstructure reactor to ensure that the furfuryl alcohol solution and the acid catalyst solution form water-in-oil two-phase droplet flow in the passage of the microstructure reactor; pre-polymerizing the formed droplet flow, keeping on reaction, and collecting reaction products; and centrifuging, cleaning, drying and heating the reaction products to obtain the carbon micron-spheres, wherein the carbon micron-spheres have narrower particle size distribution, and the average particle size is continuously adjustable between 150 and 600 mu m. The preparation process has simple operation, and the size and the wall thickness of the carbon-spheres can be adjusted conveniently.

The invention provides a high-throughput screening apparatus using a droplet micro-fluidic chip. The apparatus comprises: a rack; a micro-fluidic control system constructed to control a fluid supply device for supplying a droplet-containing fluid to the droplet micro-fluidic chip; a photoelectric detection system comprising an incident light generation device for limiting outgoing and incident light paths, a preferable laser excitation device and a fluorescence detection device for limiting a fluorescent light path; an electric screening system constructed to connect the droplet micro-fluidicchip in order to screen droplets flowing through the droplet micro-fluidic chip; and one or more controllers constructed to receive a signal from the fluorescence detection device, compare the signalwith a predetermined threshold and control the electric screening system to perform droplet screening according to the obtained comparison result.

The invention relates to the separation of microparticles by means of a nozzle (12) for the release of a separating stream (20), comprising at least one enveloping fluid and microparticles entrained Therein, with a vibration generator (18), for the dispersion of the separation flow (20) into a droplet flow (20′), with a deflector device (24), for separation of the droplet flow (20′) into a through flow (20A) and at least one side flow (20B, 20C, . . . ), depending on signals from an analytical device (38). Said analytical device (38) is provided for the analysis of the microparticles(s) contained in the droplets and to control the deflector device (24). Also provided is a protective chamber for product and personnel protection, in which at least the nozzle (12), the oscillation generator (18) and the deflector device (24) are arranged. The analytical device is arranged essentially outside the microbiological safety cabinet (200), whereby, according to the invention, the protective chamber is part of a microbiological safety cabinet (MSW 200), in particular with a laminar throughflow, with a clean chamber (34) and with a monitoring device (22), provided in a side wall, in particular in the back wall (32) of the microbiological safety cabinet (200), through which the signals of the microparticles arising from the separating stream (20) are supplied to the detectors of the analytical device (38), arranged outside the microbiological safety cabinet (200).

The invention discloses a droplet-based microfluidic chip for detecting nucleic acid in a high sensitive manner and a using method thereof. The droplet-based microfluidic chip has the front side and the back side, and comprises a sample feeding chamber, an oil phase sample feeding chamber and a second accommodating space, wherein samples pass through a microfluidic channel from the sample feedingchamber and is converged to the oil phase sample feeding chamber and the microfluidic channel; droplets are prepared and then flow into a first accommodating space; the droplets flow into the second accommodating space through a tilting chamber inlet from the first accommodating space. According to the droplet-based microfluidic chip for detecting nucleic acid in the high sensitive manner and theusing method thereof disclosed by the invention, functional areas are partitioned, three steps, including preparation of the droplets, PCR (Polymerase Chain Reaction) amplification and tilting statistical counting, preparation and amplification of the droplets, of the digital PCR are separately carried out in an individual area, micro-droplets are located in a free state, and the stability of themicro-droplets is high, so that the stability of the droplets is effectively ensured, an uncovering step of the digital PCR chip in a using process is avoided, and the crossed pollution risk is avoided, and thus, the success rate and the timeliness of implementation of the digital PCR are improved.

The invention discloses a vapor-assisted metal micro-droplet manufacturing device and method and belongs to a micro-droplet jetting additive manufacturing technology. The inside of a seal box body is kept in a closed environment to keep an internal temperature invariable; a liquid metal is stored in an inner crucible, an inert gas with pulsating pressure is introduced from a pulsating pressure gas inlet, and the liquid metal is extruded to flow outside from a liquid outlet; a certain distance is kept between an outer crucible and the inner crucible; a heater wraps the outside of the outer crucible to keep the temperature of an internal environment constant; a high-pressure gas flows from an air inlet passage and a flowing metal liquid is extruded and sheared at a liquid outlet; the high-pressure gas flows outside from an air leakage passage and generated micro-droplets flow outside from a droplet outlet and fall to a deposition platform. Through the viscous shearing action of the gas around the liquid outlet, advanced shear fracture of the liquid is achieved at a droplet formation stage, smaller uniform micro-droplets are generated, improvement of the accuracy and the compactness of a formed part is facilitated, a metal lattice structure is more uniform and the mechanical property is better.

PCT No. PCT/EP96/05043 Sec. 371 Date Jun. 12, 1998 Sec. 102(e) Date Jun. 12, 1998 PCT Filed Nov. 16, 1996 PCT Pub. No. WO97/21544 PCT Pub. Date Jun. 19, 1997A method for monitoring the amount of moisture sprayed on a moving material web or a roller across their width, with the width being divided into individual measurement areas includes a measuring step, a storing step and further measuring steps. The measurement is made of local droplet flow of the sprayed liquid in the individual measurement areas, storing the measured values as set values, and again measuring the local droplet flow and comprising it to the set values. The device used includes a counting device and an evaluation unit. The counting device registers droplets of the liquid sprayed on the moving material web in a respective one of the individual measurement areas, and generates a signal per registered droplet which is supplied to the evaluation unit.

The invention discloses a device for producing and measuring droplets on a micro-fluidic chip. The device comprises a rotating platform with adjustable rotation speed, a micro-fluidic chip and micro-flow pipes disposed on the micro-fluidic chip. The device also comprises auxiliary rotating shafts and two locking valves. The two locking valves are arranged at two sides of the micro-fluidic chip respectively. The micro-fluidic chip can rotate around the auxiliary rotating shafts. Included angles between micro-flow pipe directions and radial directions of the rotating platform are adjusted through rotation acceleration of the rotating platform. The micro-fluidic chip is locked on two states by the locking valves, so as to change droplet flow directions in the micro-flow pipes. Each micro-flow pipe comprises a first horizontal reversible pipe, a radial normal-open pipe, a second horizontal reversible pipe and a main pipe. The invention also discloses a method based on the device. By periodically switching rotation directions of the rotating platform, production and measurement of the droplets on the micro-fluidic chip are realized; a structure is simple; cost is low and operations are convenient.

The invention discloses a device and method for preparing metal microballoons by using ultrasonic surface standing waves, relates to a device and a method for preparing the metal microballoons, and aims to solve the problems that the conventional device for preparing the metal microballoons is complicated, and the size cannot be controlled flexibly. The device consists of an air storehouse and a titanium alloy casting die, a heating element, a base plate, two ultrasonic surface wave emission devices and a droplet flow control device which are positioned in the air storehouse. The method comprises the following steps of: 1, completely dissolving a metal material to obtain a metal solution, gradually dropping the metal solution on the surface of the base plate, and forming spherical metal droplets with the same size under the action of ultrasonic waves; and 2, cooling the metal microballoons by an air-cooling system until the metal microballoons are cooled to be solids, and thus obtaining the metal microballoons with the same size. By the device and the method, various metal microballoons with relatively small sizes can be prepared; the preparation process takes 1 to 2 minutes at one time, so that the production efficiency is greatly improved; and the device and the method are applied to the field of preparation of the metal microballoons.

The invention relates to a microfluidic chip and a microfluidic system. The microfluidic chip comprises a droplet flow channel, at least two grating regions, a light source and a wavelength detector,wherein grating constants of the at least two grating regions are different and the grating regions are arranged in the length direction of the droplet flow channel; when droplets are absent in the droplet flow channel, the at least two grating regions reflect light with different designated wavelengths, and when the droplets are present in the droplet flow channel, wavelength of light reflected by the grating regions opposite to the positions of the droplets is different from the designated wavelength; the light source is located at the first end of the droplet flow channel in the length direction and is used for providing incident light including the different designated wavelengths; the wavelength detector detects reflected light after the incident light passes through the grating regions when at the first end, and detects transmitted light after the incident light passes through the grating regions when at the second end opposite to the first end. According to the embodiment, detection accuracy of the droplets in the microfluidic chip can be improved, and accurate control of the droplets can be realized.

The invention relates to an electrostatic spinning device with solid needles, and belongs to the field of nano-fiber preparation. The electrostatic spinning device with the solid needles comprises a solution supply system, a solid needle spinneret system, a lifting system, a power supply system, a receiving device and a control system. According to the electrostatic spinning device with the solid needles, the solid needles are wrapped with insulating solution guide rods, so that electrostatic repulsion between the solid needles is obviously reduced; the insulating solution guide rods make close contact with conical countersunk through holes in the bottom of a spinneret solution storage box, the gaps between the insulating solution guide rods and the conical countersunk parts become large when the insulating solution guide rods move upwards under driving of the lifting system, and thus droplets flow out along the bottom cones of the insulating solution guide rods; the tips of the solid needles are excited by an electric field to produce Taylor cones, and as the insulating solution guide rods can move vertically, the blocking phenomenon in capillary needle type electrostatic spinning can be effectively avoided; the distance between each solid needle and a receiving electrode is made proper by adjusting the height of the insulating solution guide rods, the field intensity uniformity at the tips of the solid needles can be achieved, and thus the uniform spinning effect can be achieved.

The invention discloses a microfluidic reaction chip, a liquid droplet positioning control system based on the same and a control method. The microfluidic reaction chip is characterized by comprisinga digital microfluidic chip, wherein the digital microfluidic chip comprises an upper polar plate and a lower polar plate, at least three liquid droplet flow channels are formed between the upper polar plate and the lower polar plate, and a reaction tank is formed at the intersection of the liquid droplet flow channels; a titration hole is formed in the upper part of each liquid droplet flow channel and penetrates the upper polar plate; at least one of the liquid droplet flow channel is an output flow channel, and the others are input flow channels. The liquid droplet flow channels of the digital microfluidic chip are filled with black liquid insoluble with liquid droplets, an infrared laser module and a light intensity sensing module are added to the two ends of the digital microfluidic chip, a driving process of micro-droplets can be observed clearly and accurately, and success rate of complicated micro-droplet transportation and application is greatly increased.