The invention discloses a droplet-based
microfluidic chip for detecting
nucleic acid in a
high sensitive manner and a using method thereof. The droplet-based
microfluidic chip has the front side and the back side, and comprises a sample feeding chamber, an
oil phase sample feeding chamber and a second accommodating space, wherein samples pass through a
microfluidic channel from the sample feedingchamber and is converged to the
oil phase sample feeding chamber and the
microfluidic channel; droplets are prepared and then flow into a first accommodating space; the droplets flow into the second accommodating space through a tilting chamber inlet from the first accommodating space. According to the droplet-based
microfluidic chip for detecting
nucleic acid in the
high sensitive manner and theusing method thereof disclosed by the invention, functional areas are partitioned, three steps, including preparation of the droplets, PCR (
Polymerase Chain Reaction) amplification and tilting statistical counting, preparation and amplification of the droplets, of the digital PCR are separately carried out in an individual area, micro-droplets are located in a
free state, and the stability of themicro-droplets is high, so that the stability of the droplets is effectively ensured, an uncovering step of the digital
PCR chip in a using process is avoided, and the crossed
pollution risk is avoided, and thus, the success rate and the timeliness of implementation of the digital PCR are improved.